Details of cellular interaction with a fibronectin coated surface were examined using surface plasmon resonance imaging (SPRI). SPRI is a label-free highly sensitive technique that is based on the measurement of interfacial refractive index. SPRI is uniquely sensitive to short range interactions between cells and their substrate. With this novel technique, we demonstrate visualization of cell-substrate interactions and how cell-substrate interactions change over time. The high contrast in SPR signal between cell edges and substrate facilitates identification of cell edges and segmentation of cell areas. SPRI was used to follow live cells as they spread, migrated, and exhibited membrane ruffling.
Cytometry Part A