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Bioscience

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Displaying 701 - 725 of 1261

Three-dimensional Shape Metrology for Tissue Engineering

April 21, 2012
Author(s)
Joy P. Dunkers, Antonio M. Possolo, Thomas V. Lafarge, Beatriz Pateiro-Lopez, Jeffrey Coles, Carl G. Simon Jr.
The physical and chemical properties of a tissue scaffold influences cell response and therefore the success of the resulting tissue construct. Scaffold structure from nanometers to millimeters can direct cell behavior, from viability to proliferation to

Positively-charged Dimethacrylates to Reduce Bacterial Attachment

March 30, 2012
Author(s)
Nancy J. Lin, Joseph M. Antonucci, Diana N. Zeiger, Sheng Lin-Gibson, Kathy Tang
The widespread incidence of recurrent tooth decay (caries) highlights the need for improved dental restorative materials. Caries are most frequently caused by acid-producing bacterial biofilms. One approach for developing biomaterials with reduced

Tumor Necrosis Factor Interaction with Gold Nanoparticles

March 14, 2012
Author(s)
De-Hao D. Tsai, Sherrie R. Elzey, Frank W. DelRio, Robert I. MacCuspie, Suvajyoti S. Guha, Michael R. Zachariah, Athena M. Keene, Jeffrey D. Clogston, Vincent A. Hackley
We report on a systematic investigation of molecular conjugation of tumor necrosis factor protein-α (TNF) onto gold nanoparticles (AuNPs) and the subsequent binding behavior to its antibody (anti-TNF). We employ a combination of physical and spectroscopic

The Biological Macromolecule Crystallization Database

March 6, 2012
Author(s)
David Travis Gallagher, Michael Tung
The Biological Macromolecule Crystallization Database (BMCD) is described. The database currently contains 14372 entries with crystallization information for proteins, protein–protein complexes, nucleic acids, nucleic acid–nucleic acid complexes, protein

Challenges in Microbial Sampling in the Indoor Environment

March 1, 2012
Author(s)
Jayne B. Morrow, Autumn S. Downey, Jordan Peccia
This report summarizes the results of the Challenges in Microbial Sampling in the Indoor Environment Workshop held on February 14-15, 2011 at the National Institute of Standards and Technology in Gaithersburg, Maryland.

Freeform Fabricated Scaffolds with Roughened Struts that Enhance both Stem Cell Proliferation and Differentiation by Controlling Cell Shape

March 1, 2012
Author(s)
Girish Kumar, Carl G. Simon Jr., Michael S. Waters, Tanya M. Farooque, Marian F. Young
We demonstrate that imparting freeform fabricated (FFF) scaffolds with surface roughness on their struts enhances osteogenic differentiation of primary human bone marrow stromal cells (hBMSCs) by controlling cell shape. Previous work showed that hBMSCs

Preparation and Value Assignment of Standard Reference Material 968e Fat-Soluble Vitamins, Carotenoids, and Cholesterol in Human Serum

January 2, 2012
Author(s)
Jeanice M. Brown Thomas, David L. Duewer, Lane C. Sander, Katherine E. Sharpless, Lorna T. Sniegoski, Michael J. Welch, James H. Yen, Mugenya O. Isaac
Standard Reference Material 968e Fat-Soluble Vitamins, Carotenoids, and Cholesterol in Human Serum provides certified values for total retinol, γ- and α-tocopherol; total lutein, total zeaxanthin, total β-cryptoxanthin, total β-carotene, and cholesterol in

New Concepts for Building Vocabulary for Cell Image Ontologies

December 21, 2011
Author(s)
Anne L. Plant, John T. Elliott, Talapady N. Bhat
Background: We present an approach to cell image databasing that is compatible with searching across a global federation of independent image databases. The variety of biological experiments and data that practitioners would like to access and share is

How well do you know your MATES???

December 1, 2011
Author(s)
Joy P. Dunkers
There is a group that quietly goes about the business of advancing the areas of tissue engineering and regenerative medicine. I say quietly because their work, while impactful, is not well known outside of government circles. How well do you know your

EVALUATION OF A MICROWAVE-ASSISTED METAL-LABELING STRATEGY FOR QUANTIFICATION OF PEPTIDES VIA RPLC-ICP-MS AND THE METHOD OF STANDARD ADDITIONS

November 26, 2011
Author(s)
Steven J. Christopher, Eric L. Kilpatrick, Lee L. Yu, William C. Davis, blakely adair
NIST has performed preliminary research on applying a calibration methodology based on the method of standard additions to the quantification of lanthanide-labeled peptides via reverse-phase liquid chromatography coupled to inductively coupled plasma mass

Protein characterization of a candidate mechanism SNP for Crohn's disease: the macrophage stimulating protein R689C substitution

November 7, 2011
Author(s)
Illarion V. Turko, Natalia Gorlatova, Kinlin L. Chao, Lipika Ray, Rawan Araj, Andrey Galkin, John Moult, Osnat Herzberg
Macrophage Stimulating Protein (MSP) is the ligand of RON receptor tyrosine kinase. MSP binding to RON activates a signaling pathway that plays a dual role in inflammation, initially stimulating macrophage chemotaxis and phagocytosis, but also changing

The Latest and Greatest NIST PCR-based DNA Profiling Standard: Updates and Status of Candidate Standard Reference Material (SRM) 2391c

November 1, 2011
Author(s)
Margaret C. Kline, Carolyn R. Steffen, Jamie L. Almeida, Erica L. Romsos, Michael D. Coble, John M. Butler
Standard Reference Material 2391c (SRM 2391c) PCR-based DNA Profiling Standard is the third renewal of this SRM, originally released in 1995. SRM 2391c consists of 6 candidate components labeled A through F. Components A through D are supplied as genomic

Morphological Changes Driven by Nanofibrous Scaffolds Induce Marrow Stromal Cell Osteogenesis

October 11, 2011
Author(s)
Carl G. Simon Jr., Kaushik Chatterjee, Christopher K. Tison, Girish Kumar, Patrick S. Pine, Marc L. Salit, Jennifer H. McDaniel, Marian F. Young
Cells are sensitive to tissue scaffold architecture and these cell-material interactions drive cell functions critical in tissue regeneration. Results presented here demonstrate that nanofiber scaffolds force primary human bone marrow stromal cells (hBMSCs
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