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Young Jong Lee (Fed)

Research Chemist, Project Leader

Since Young Jong Lee joined NIST in 2006, he has been developing new hyperspectral imaging and spectroscopy technologies based on coherent Raman and infrared (IR) absorption to measure the quantitative and structural information of complex materials and biological systems. His recent research has been focused on new spectroscopic methods for high-sensitivity characterization of higher-order structures of protein. He also works on the development of next-generation IR- and Raman-based microscopic techniques for live cells and semicrystalline polymers. (


Development of New Measurement Technology

Therapeutic Proteins

Soft Matter Physics

Cell Systems and Tissues

  • Label-free functional imaging of stem cells in scaffolds for regenerative medicine
  • In-situ, non-invasive spectroscopic imaging of biofilms


Postdocs or Guest Researchers:  open to either non-US citizens or US citizens. If interested in any projects described above, please send an email to youngjong.lee [at]

NRC Postdoctoral Fellowship: US citizens only. Please visit the following opportunities.

(left) Label-free chemical imaging of stem cell differentiation by BCARS (AD: adipogenic, and OS: osteogenic) in Tissue Eng. Part C, 20, 562 (2014). (top center) BCARS composite image of a polyethylene blend (h-LLDPE: red; and d-HDPE: green) in ACS Macro Lett. 1, 1347 (2012). (top right) Polarization-controlled IR absorption image of a semicrystalline PCL film. (bottom right) QCL-IR absorption spectra of NISTmAb and BSA solutions in Anal. Chem. 93, 2215 (2021).


  • S. Xu, C. H. Camp Jr. Y. J. Lee, Coherent anti-Stokes Raman scattering (CARS) microscopy for polymers, J. Polym. Sci. in press (2021).
  • B. Chon, S. Xu, Y. J. Lee, Compensation of Strong Water Absorption in Infrared Spectroscopy Reveals the Secondary Structure of Proteins in Dilute Solutions, Anal. Chem. 93, 2215 (2021).
  • B. Chon, J. Truong, M. Hansen, J. Hahm, Y. J. Lee, Position- and Polarization-Specific Waveguiding of Multi-Emissions in Single ZnO Nanorods, ACS Photonics 6, 1416 (2019).
  • Y. J. Lee, Concurrent Polarization IR Analysis to Determine the 3D Angles and the Order Parameter for Molecular Orientation Imaging, Opt. Express 26, 24577 (2018).
  • Y. Jin, A. P. Kotula, A. R. Hight Walker, K. B. Migler, Y. J. Lee, Raman Identification of Multiple Melting Peaks of Polyethylene, Macromolecules 50, 6174 (2017).
  • Y. J. Lee, Least Squares Moving-Window Spectral Analysis, Appl. Spectrosc. 71, 1894 (2017).
  • I. S. Ryu, C. H. Camp Jr., Y. Jin, M. T. Cicerone, Y. J. Lee, Beam Scanning for Rapid Coherent Raman Hyperspectral Imaging, Opt. Lett. 40, 5826 (2015).
  • Y. J. Lee, S. L. Vega, P. J. Patel, K. A. Aamer, P. V. Moghe, M. T. Cicerone, Quantitative, Label-Free Characterization of Stem Cell Differentiation at the Single-Cell Level by Broadband Coherent Anti-Stokes Raman Scattering Microscopy, Tissue Eng. Part C 20, 562 (2014).
  • Y. J. Lee, C. R. Snyder, A. M. Forster, M. T. Cicerone, W. Wu, Imaging the Molecular Structure of Polyethylene Blends with Broadband Coherent Raman Microscopy, ACS Macro Lett. 1, 1347 (2012).
  • Y. J. Lee, S. H. Parekh, F. A. Fagan, M. T. Cicerone, Phonon Dephasing and Population Decay Dynamics of the G-band of Semiconducting Single-Wall Carbon Nanotubes. Phys. Rev. B 82, 165432 (2010).
  • Y. J. Lee, Y. Liu, M. T. Cicerone, Characterization of 3-Color CARS in a 2-Pulse Broadband CARS Spectrum. Opt. Lett. 32, 3370 (2007).



This is a line drawing for patent number 10,625,470

3D Printing of Composition-Controlled Copolymers

NIST Inventors
Young Jong Lee and
Patent Description This invention is a new 3D printing process of continuously or discretely controlling composition of photo-polymerization reactants to produce 3D objects with spatially varying chemical, physical and mechanical properties. This invention is based on photo­ polymerization of liquid
Schematic comparison of the conventional approach and this invention for a sample (analyte + solvent) with a strong solvent absorption band in the spectral range of interest.

Spectrum Adjuster and Producing a Pure Analyte Spectrum

NIST Inventors
Young Jong Lee
Patent Description This invention is a spectroscopic method that adjusts the incident light intensity as a function of wavelength to flatten the solvent-only transmitted light spectrum over an interesting wavelength range. When a solvent has a strong absorption band within an interesting spectral
Created July 30, 2019, Updated September 1, 2021