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Young Jong Lee (Fed)

Research Chemist, Project Leader

Since Young Jong Lee joined NIST in 2006, he has developed new microscopy and spectroscopy technologies based on coherent Raman and infrared (IR) absorption to measure the quantitative and structural information of complex materials and biological systems. His recent research has focused on developing new IR spectroscopic methods for the high-sensitivity characterization of proteins and new microscopic methods based on IR and Raman-based for live cells and polymers. (


Development of New Measurement Technology

Therapeutic Proteins

Soft Matter Physics

Cell Systems and Tissues


Postdocs: positions may be occasionally available. Both non-US citizens and US citizens. If interested in any projects described above, please send an email to youngjong.lee [at]

NRC Postdoctoral Fellowship: US citizens only. Please visit the following opportunities.

(left) Label-free chemical imaging of stem cell differentiation by BCARS (AD: adipogenic, and OS: osteogenic) in Tissue Eng. Part C (2014). (top center) BCARS composite image of a polyethylene blend (h-LLDPE: red; and d-HDPE: green) in ACS Macro Lett. (2012). (top right) Polarization-controlled IR absorption image of a semicrystalline PCL film in Opt. Express (2022). (bottom right) QCL-IR absorption spectra of NISTmAb and BSA solutions in Anal. Chem. (2021).


  • S. Xu, J. Rowlette, Y. J. Lee, Imaging 3D molecular orientation by orthogonal-pair polarization IR microscopy, Opt. Express 30, 8436 (2022).
  • B. Chon, S. Xu, Y. J. Lee, Compensation of Strong Water Absorption in Infrared Spectroscopy Reveals the Secondary Structure of Proteins in Dilute Solutions, Anal. Chem. 93, 2215 (2021).
  • Y. Jin, A. P. Kotula, A. R. Hight Walker, K. B. Migler, Y. J. Lee, Raman Identification of Multiple Melting Peaks of Polyethylene, Macromolec. 50, 6174 (2017).
  • I. S. Ryu, C. H. Camp Jr., Y. Jin, M. T. Cicerone, Y. J. Lee, Beam Scanning for Rapid Coherent Raman Hyperspectral Imaging, Opt. Lett. 40, 5826 (2015).
  • Y. J. Lee, S. L. Vega, P. J. Patel, K. A. Aamer, P. V. Moghe, M. T. Cicerone, Quantitative, Label-Free Characterization of Stem Cell Differentiation at the Single-Cell Level by Broadband Coherent Anti-Stokes Raman Scattering Microscopy, Tissue Eng. Part C 20, 562 (2014).
  • Y. J. Lee, C. R. Snyder, A. M. Forster, M. T. Cicerone, W. Wu, Imaging the Molecular Structure of Polyethylene Blends with Broadband Coherent Raman Microscopy, ACS Macro Lett. 1, 1347 (2012).



This is a line drawing for patent number 10,625,470

3D Printing of Composition-Controlled Copolymers

NIST Inventors
Young Jong Lee
Patent Description This invention is a new 3D printing process of continuously or discretely controlling composition of photo-polymerization reactants to produce 3D objects with spatially varying chemical, physical and mechanical properties. This invention is based on photo­ polymerization of liquid
Schematic comparison of the conventional approach and this invention for a sample (analyte + solvent) with a strong solvent absorption band in the spectral range of interest.

Spectrum Adjuster and Producing a Pure Analyte Spectrum

NIST Inventors
Young Jong Lee
Patent Description This invention is a spectroscopic method that adjusts the incident light intensity as a function of wavelength to flatten the solvent-only transmitted light spectrum over an interesting wavelength range. When a solvent has a strong absorption band within an interesting spectral
Created July 30, 2019, Updated October 24, 2022