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Bioscience

Projects/Programs

Keyword

Organizational Unit

Gene Delivery Particle Analysis with High-Throughput Light Scattering Microscopy

Ongoing

This project aims to develop light-scattering microscopy methods for rapid, multi-attribute characterization of gene therapy particles at the single particle level. Emerging gene therapy treatments use gene delivery particles, such as viral vectors and non-viral carriers, to cure intractable

Gene Delivery Systems

Ongoing

Viral Vectors Viral vectors are a critical tool for delivering DNA or RNA into patient’s cells. Common viral vectors for gene therapy include adenovirus, lentivirus, and adeno associated virus. Administration of viral vectors can occur in vivo or ex vivo , such as for adeno associated virus or

Genome in a Bottle

Ongoing

Consortium goals: The Genome in a Bottle Consortium is a public-private-academic consortium hosted by NIST to develop the technical infrastructure (reference standards, reference methods, and reference data) to enable translation of whole human genome sequencing to clinical practice and innovations

Genome in a Bottle Workshops

Ongoing

GIAB has held 1-2 workshops each year since being formed in 2012. These workshops include progress updates, presentations about related work, and planning for new samples and analyses. Following are links to agendas, reports, and presentations from past workshops. Workshop Reports APRIL 2020

Glycan Standard Reference Materials

Ongoing

Now Available A glycan-based NIST Standard Reference Material ( SRM 3655 Glycans in Solution) supports the biomanufacturing and protein therapeutics communities with a set of 13 individually-bottled, pure glycoforms including those most commonly observed as N-linked glycans on therapeutic monoclonal

HDX-MS for Biopharmaceutical Analysis

Ongoing

Intended Impact This project is designed to improve, test, and validate hydrogen/deuterium exchange mass spectrometry (HDX-MS) metrology for the determination of dynamical properties of therapeutic proteins and glycoproteins. Objectives Explore structure-function relationships between glycan

High-Precision Isotopic Reference Materials

Ongoing

A new generation of multi-collector IRMS, utilizing both inductively coupled plasma and thermal ionization sample sources, is now capable of measuring isotope ratios with extremely high precision – a few parts in 10 6. This new measurement capability has caused a minor revolution in the use of

High-Sensitivity IR Spectroscopy of Proteins

Ongoing

Infrared (IR) absorption spectroscopy has been widely used as a non-invasive, label-free characterization method for the chemical identification and structure of complex biomolecules. Fourier-transform IR (FT-IR) technology is commonly used to characterize proteins and other biological molecules

Human Gut Microbiome Reference Material

Ongoing

The most common measurements currently being used to describe these complex microbial communities are: NGS-based metagenomics Mass spectrometry-based metabolomics In either case, no fit-for-purpose standards exist that enable researchers to compare results generated across different laboratories and

Improved Benchtop Connectivity to Complex Microfluidic Devices

Completed

Intended impact Microfluidic devices with many fluidic and pneumatic inlets are difficult to connect to benchtop reservoirs and controllers. Many hours can be spent sorting, untangling, and connecting tubing to a single device. If one then finds that the device is not fully functional, the process

H5 Influenza Positive Controls

Ongoing

In response to the current H5N1 avian influenza outbreak, NIST has developed a research grade test material (RGTM) for PCR-based assays. This material contains 3 vials with RNA fragments for HA (Part A), NA (Part B), and MP (Part C). A NIST Reference Material is also under development. The guidance

Integrated Lentiviral Vector Copy Number Measurements: Invitation to Participate in an Interlaboratory Testing Program Evaluating Genomic DNA Test Materials

Ongoing

NIST has produced genomic DNA test materials from transduced Jurkat cell lines with integrated lentiviral vector copy number (VCN) for evaluation as a candidate reference material. Transducing Jurkat (Clone E6-1) cell lines with a lentivirus expressing a green fluorescent protein were produced by

Interdomain Distance Measurements of Monoclonal Antibodies

Ongoing

Through collaboration with researchers at the Institute for Biotechnology and Bioscience Research (IBBR), we have created a library of site-specific spin-labeled and stable isotope-labeled binding partner proteins for the NIST monoclonal antibody (NISTmAb) reference material ( RM8671). Using pulsed

Label-free imaging of cells and their extracellular matrix by SPR imaging

Ongoing

Cellular remodeling of their neighboring environment, extracellular matrix (ECM), is an important biological process from development biology, to wound healing, to diseases and cancers – this is a challenging process to measure and quantify. Surface plasmon resonance imaging (SPRI) has been

LANTERN - Interpretable modeling of genotype-phenotype landscapes with state-of-the-art predictive power

Ongoing

LANTERN: genotype-phenotype LANdscape in TERpretable Non-parametric model The bioeconomy is increasingly data-driven, relying on large-scale measurements to rapidly engineer novel functions. To facilitate rational engineering, where complex designs are constructed from well understood components

Large-Scale Genotype-Phenotype Landscape Measurements for Precision Engineering of Living Measurement Systems

Ongoing

Synthetic biology promises an array of world-changing innovations including living therapeutics that fight infections and cancer or even regrow whole organs in situ; cellular factories that weave materials with nanoscale features; and synthetic ecosystems that produce fertilizer and pesticides on

Live Cell Imaging of Induced Pluripotent Stem Cell Populations

Ongoing

As part of our program in Cell Biology , our live cell image program supports the advancement of induced pluripotent stem cell (iPSC) technology in three ways: 1) Identification of process control measurements: A critical component to the translation of iPSCs into therapeutic applications is to

Living Yeast Cell Reference Material (RM 8230)

Ongoing

Quantification of total and viable microbial cells is critical for applications ranging from assessment of DNA extraction efficiency in support of microbial metagenomics to characterization of antimicrobial efficacy. In addition, safe biological materials are needed to substitute for true biothreat

Cryogenic electron microscopy (cryo-EM) image of an LNP/RNA formulation

LNP-RNA Research Grade Test Material

Ongoing

The currently planned LNP/RNA material (designated RGTM 10240) will include a fragment of the SARS-CoV-2 genome that is approximately 4 kb in length. This fragment was previously included (in unencapsulated form) as “Fragment 1” of NIST RGTM 10169. The RNA fragment is not designed for cell-based

Magnetic Resonance Imaging (MRI) Biomarker Measurement Service

Ongoing

Biomarkers are parameters that can be quantitatively measured in-vivo and provide information on tissue health/ pathology. Phantoms are medical imaging calibration structures designed to test scanner performance, image quality, and image-based measurements.

Magnetic Sensing and Metrology

Ongoing

Magnetic sensors have a wide range of sensitivities, spatial resolution, dynamic range, bandwidths, size, and cost. Applications such as magnetic data storage and chip NDE require sensitivity of 1 nT, spatial resolution down to a few nanometers and bandwidths up to 10 GHz, while MEG requires

MML researchers develop reference materials and methods for quantitative phase microscopy with off-the-shelf products

Measurement Assurance for Quantitative Cell Imaging by Optical Microscopy

Ongoing

Light microscopy is unparalleled as a quantitative technique for studying cell biology, but assuring that the results of measurements and analyses are accurate, repeatable and sharable is challenging. Here we provide information on reference materials, protocols and approaches for evaluating the

Measurement of Low Frequency Mutations in Circulating Tumor DNA (ctDNA) through Interlaboratory Testing

Completed

A very exciting development in the diagnosis and therapeutic monitoring of cancer is the discovery that blood and urine contain nucleic acids derived from cancer cells that can be used to as biomarkers. DNA, RNA, and microRNA derived from tumor cells can be measured with high sensitivity techniques

Measurements for minimal cells

Ongoing

Minimal cells are cells whose genome has been stripped of all but those genes essential for replication, in a process called "genome minimization." NIST and collaborators demonstrated genome minimization coupled to single cell imaging as a powerful approach to investigate the minimal genetic

Measurements of Point-Defect Chemistry in Complex Oxides

Ongoing

Project Goal: To develop magnetic resonance, x-ray absorption, electron diffraction, and electrical conductivity measurements to better characterize dilute concentrations of point defects in oxide materials and effectively correlate electro-mechanical properties to measured defect chemistry. Oxide

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