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Search Publications by: Jeeseong C. Hwang (Fed)

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Displaying 26 - 50 of 84

An Average Enumeration Method of Hyperspectral Imaging Data for Quantitative Evaluation of Medical Device Surface Contamination

September 15, 2014
Author(s)
Hanh Le, Moon Kim, Jeeseong C. Hwang, Yi Yang, Pawneena U-Thainual, Jin Kang, Do-Hyun Kim
We propose a quantification method called Mapped Average Principal component analysis Score (MAPS) to enumerate the contamination coverage on common medical device surfaces. The method was adapted from conventional Principal Component Analysis (PCA) on non

A Candidate Reference Analytical Method for Quantification of Low Concentrations of Plasmid DNA by Exhaustive Counting of Single DNA Molecules in a Flow Stream

August 4, 2014
Author(s)
Hee-Bong Yoo, Dong-Geun Oh, Jae Yong Song, Mamoru Kawaharasaki, Jeeseong C. Hwang, In Chul Yang, Sang-Ryoul Park
This work demonstrates accurate measurement of the concentration of low-level plasmid DNA by counting individual DNA molecules using a high-sensitivity flow cytometric setup. Plasmid DNA is a widely used form of DNA, and its quantity often needs to be

Optical Coherence Tomography for Dimensional Metrology of Lab-on-a-chip devices

October 27, 2013
Author(s)
Darwin R. Reyes-Hernandez, Michael W. Halter, Jeeseong Hwang
Here we present a new metrology tool for the determination of the dimensions of channels from final product Lab-on-a-chip devices. Using Optical Coherence Tomography (OCT) areas of up to 12 mm x 12 mm can be imaged in less than 1 second, without the need

Mapping the Vif-A3G interaction using peptide arrays: a basis for anti-HIV lead peptides

June 15, 2013
Author(s)
Jeeseong Hwang, Ji Y. Lee, Abigail E. Miller, Tali Reingewertz, Elena Britan-Rosich, Shahar Rotem-Bamberger, Mathias Viard, Robert Blumenthal, Assaf Friedler
Human apolipoprotein-B mRNA-editing catalytic polypeptide-like 3G (A3G) is a cytidine deaminase that restricts retroviruses, endogenous retro-elements and DNA viruses. A3G plays a key role in the anti- HIV-1 innate cellular immunity. The HIV-1 Vif protein

Metrology for Lab-on-a-Chip Final-Product Devices

May 12, 2013
Author(s)
Darwin R. Reyes-Hernandez, Michael W. Halter, Jeeseong Hwang
New metrology tools to measure the critical parameters of internal structures in lab on a chip devices are greatly needed in order to develop standard tests for this technology. Here we present a method that combines a custom made optical coherence

Developing digital tissue phantoms for hyperspectral imaging of ischemic wounds

May 18, 2012
Author(s)
David W. Allen, Jeeseong Hwang, Maritoni A. Litorja, Joseph P. Rice
Biomedical optical imaging has the potential to achieve high spatial resolution and high functional sensitivity for non-invasive assessment of ischemic wounds. However, clinical acceptance of many optical imaging devices is hampered by poor reproducibility

Algorithm Validation Using Multicolor Phantoms

May 9, 2012
Author(s)
Daniel V. Samarov, Matthew L. Clarke, Ji Y. Lee, David W. Allen, Maritoni A. Litorja, Jeeseong Hwang
We present a framework for hyperspectral image (HSI) analysis validation, specifically abundance fraction estimation based on HSI measurements of water soluble dye mixtures printed on microarray chips. In our work we focus on the performance of two

Designing Microarrays Phantoms for Hyperspectral Imaging Validation

May 9, 2012
Author(s)
Matthew L. Clarke, Ji Y. Lee, Daniel V. Samarov, David W. Allen, Maritoni A. Litorja, Ralph Nossal, Jeeseong Hwang
The simple design of custom-tailored microarrays for the characterization of hyperspectral imaging systems and analysis methods for biologically relevant samples. Individual and mixtures of dyes were printed on microscope slides and imaged by a

Hyperspectral Image Projector Applications

February 15, 2012
Author(s)
Joseph P. Rice, Steven W. Brown, David W. Allen, Howard W. Yoon, Maritoni A. Litorja, Jeeseong Hwang
For the past several years NIST has been developing, along with several collaborators, a Hyperspectral Image Projector (HIP). This scene projector produces high-resolution programmable spectra and projects them into dynamic two-dimensional images. The

Validating the LASSO Algorithm by Unmixing Spectral Signatures in Multicolor Phantoms

February 1, 2012
Author(s)
Daniel V. Samarov, Matthew L. Clarke, Ji Y. Lee, David W. Allen, Maritoni A. Litorja, Jeeseong Hwang
As hyperspectral imaging (HSI) sees increased implementation into the biological and medical fields it becomes increasingly important that the algorithms being used to analyze the corresponding output be validated. While certainly important under any

Hyperspectral Imaging and Analysis of Single Erythrocytes

August 12, 2011
Author(s)
Ji Y. Lee, Matthew L. Clarke, Fuyuki Tokumasu, John F. Lesoine, David W. Allen, Robert C. Chang, Maritoni A. Litorja, Jeeseong Hwang
We report a hyperspectral imaging and analysis technique to resolve unique physico-chemical characteristics of subcellular substances in single erythrocytes. We constructed a microscope system installed with a spectral light engine capable of controlling

Real-time polarization microscopy for probing local distributions of biomolecules

February 28, 2011
Author(s)
Jeeseong Hwang, Ji Y. Lee, John F. Lesoine, Hyeong G. Kang, Matthew L. Clarke, Robert C. Chang
We present real-time, full-field, fluorescence polarization microscopy and its calibration and validation methods to monitor the absorption dipole orientation of fluorescent molecules. A quarter-wave plate, in combination with a liquid crystal variable

Characterization of Hyperspectral Imaging and Analysis via Microarray Printing of Dyes

February 22, 2011
Author(s)
Matthew L. Clarke, Maritoni A. Litorja, David W. Allen, Daniel V. Samarov, Jeeseong Hwang
The application of hyperspectral imaging requires rigorous characterization of the spatial and spectral imaging domains of the system. We present a microarray printing methodology for the testing of absorption or reflectance microscopy measurements. This

System-independent assessment of OCT axial resolution with a “bar chart” phantom

February 10, 2011
Author(s)
Robert C. Chang, Jeeseong Hwang, Christopher M. Stafford, Anant Agrawal, T. Joshua Pfefer, Megan Connors
We present a novel optical phantom approach for the characterization of OCT axial resolution and contrast via multilayered “bar charts.” We explored two methods to fabricate these phantoms: the first is based on a combinatorial methods approach from

Structural Analysis of Soft Multicomponent Nanoparticle Clusters Using Electrospray Differential Mobility Analysis with Transmission Electron Microscopy

October 22, 2010
Author(s)
Leonard F. Pease , Jeremy Feldblyum, Silvia H. DePaoli Lacaerda, Rajesekhar Anumolu, Peter Yim, Matthew L. Clarke, Hyeong G. Kang, Jeeseong C. Hwang
Rapid and quantitative techniques are essential to analyze soft multicomponent nanoparticle clusters. Here we combine electrospray differential mobility analysis (ES-DMA), which measures the size of the entire cluster, with transmission electron microscopy

Shell and ligand-dependent blinking of CdSe-based core/shell nanocrystals

July 7, 2010
Author(s)
Bonghwan Chon, Sung Jun Lim, Wonjung Kim, Hyeong G. Kang, Taiha Joo, Jeeseong C. Hwang, Seung Koo Shin
Blinking of zinc blende CdSe-based core/shell nanocrystals is studied as a function of shell materials and surface ligands. CdSe/ZnS, CdSe/ZnSe/ZnS and CdSe/CdS/ZnS core/shell nanocrystals are prepared by colloidal synthesis and six monolayers of larger

Monitoring Photothermally Excited Nanoparticles via Multimodal Microscopy

May 19, 2010
Author(s)
Matthew L. Clarke, Shin G. Chou, Jeeseong Hwang
Generation of heat using optically excited nanoparticles can be beneficial or detrimental depending on the application. Therefore, clinically applicable studies are being pursued in an effort to achieve safe practices of nanoparticle-induced hyperthermia

Shell-dependent Blinking of CdSe-Based Core/Shell Nanocrystals

May 10, 2010
Author(s)
Seung Koo Shin, Bonghwan Chon, Sung Jun Lim, Wonjung Kim, Hyeong G. Kang, Taiha Joo, Jeeseong C. Hwang
Blinking of zinc-blende CdSe-based core/shell nanocrystals are studied as a function of shell materials. CdSe/ZnS, CdSe/ZnSe/ZnS, and CdSe/CdS/ZnS core/shell nanocrystals are prepared by epitaxial growth of two-to-six monolayers (MLs) of shell materials