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Metrology for Lab-on-a-Chip Final-Product Devices



Darwin R. Reyes-Hernandez, Michael W. Halter, Jeeseong Hwang


New metrology tools to measure the critical parameters of internal structures in lab on a chip devices are greatly needed in order to develop standard tests for this technology. Here we present a method that combines a custom made optical coherence tomography (OCT) system with an in-house written ImageJ macro to analyze internal structures of poly(methyl methacrylate) (PMMA) final product devices. Dimensional information was obtained from OCT images and compared with images acquired using confocal reflectance and confocal fluorescence microscopies. Although there is no established method for dimensional measurements of lab on a chip final product devices when OCT and confocal fluorescence microscopy, a well established technique for measuring fluorescent fluids within microchannels, were compared the values obtained from both techniques were remarkably similar.
Proceedings Title
Proceedings of NanoTech Conference 2013
Conference Dates
May 12-16, 2013
Conference Location
National Harbor, MD
Conference Title
Nanotech Conference 2013


dimensional metrology, lab on a chip, microfluidic devices, PMMA, optical coherence tomography, confocal fluorescence microscopy


Reyes-Hernandez, D. , Halter, M. and Hwang, J. (2013), Metrology for Lab-on-a-Chip Final-Product Devices, Proceedings of NanoTech Conference 2013, National Harbor, MD (Accessed June 22, 2024)


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Created May 12, 2013, Updated February 19, 2017