Clarke, M.
, Lee, J.
, Samarov, D.
, Allen, D.
, Litorja, M.
, Nossal, R.
and Hwang, J.
(2012),
Designing Microarrays Phantoms for Hyperspectral Imaging Validation, Biomedical Optics Express (Accessed May 29, 2026)
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Designing Microarrays Phantoms for Hyperspectral Imaging Validation
Published
Author(s)
, , , , , Ralph Nossal,
Abstract
The simple design of custom-tailored microarrays for the characterization of hyperspectral imaging systems and analysis methods for biologically relevant samples. Individual and mixtures of dyes were printed on microscope slides and imaged by a hyperspectral imaging microscope. Analysis of the spectral signatures revealed scattering and absorbance components. Separation of these signals allowed for spectral fitting of the absorbance spectra for the determination of local dye concentration.Citation
Biomedical Optics Express
Pub Type
Journals
Keywords
microarray, two-component mixtures, multivariate, dye mixture, spectroscopy, hyperspectral imaging
Citation
Issues
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Created May 9, 2012, Updated February 19, 2017