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Designing Microarrays Phantoms for Hyperspectral Imaging Validation



Matthew L. Clarke, Ji Y. Lee, Daniel V. Samarov, David W. Allen, Maritoni A. Litorja, Ralph Nossal, Jeeseong Hwang


The simple design of custom-tailored microarrays for the characterization of hyperspectral imaging systems and analysis methods for biologically relevant samples. Individual and mixtures of dyes were printed on microscope slides and imaged by a hyperspectral imaging microscope. Analysis of the spectral signatures revealed scattering and absorbance components. Separation of these signals allowed for spectral fitting of the absorbance spectra for the determination of local dye concentration.
Biomedical Optics Express


microarray, two-component mixtures, multivariate, dye mixture, spectroscopy, hyperspectral imaging


Clarke, M. , Lee, J. , Samarov, D. , Allen, D. , Litorja, M. , Nossal, R. and Hwang, J. (2012), Designing Microarrays Phantoms for Hyperspectral Imaging Validation, Biomedical Optics Express (Accessed June 24, 2024)


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Created May 9, 2012, Updated February 19, 2017