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Search Publications

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Displaying 1 - 25 of 136

Electrospray Differential Mobility Analysis as an Orthogonal Tool to Size Exclusion Chromatography for Characterization of Protein Aggregates

March 12, 2012
Author(s)
Suvajyoti S. Guha, Joshua R. Wayment, Michael J. Tarlov, Michael R. Zachariah
… and characterized using size exclusion chromatography (SEC) and ES-DMA. Although ES-DMA is a gas phase … to be in excellent agreement with those determined by SEC. It is also found that ES-DMA is more capable of resolving aggregates of IgGs than SEC. The higher resolution of ES-DMA and a limit of detection …

Determining the core, corona, and total size of CdSeS/ZnS quantum dots by SEC/QELS and TEM

May 21, 2016
Author(s)
Leena M. Pitkanen, Andre Striegel
… with on-line quasi- elastic light scattering (SEC/QELS). Accurate determination of the size of QDs is … these materials are size dependent. A clear advantage of SEC/QELS over many batch techniques (e.g., QELS without … average of the sizes present in the sample. Here, the SEC/QELS-determined RH values of CdSeS/ZnS QDs are compared …

AQUEOUS SIZE-EXCLUSION CHROMATOGRAPHY OF POLYELECTROLYTES ON REVERSED-PHASE AND HYDROPHILIC INTERACTION CHROMATOGRAPHY COLUMNS

January 12, 2018
Author(s)
Anna Caltabiano, Joe Foley, Andre Striegel
… also investigated. Non-size-exclusion chromatography (non- SEC) effects can be minimized by the addition of an … elution of the NaPSS polyelectrolyte is by a near-ideal SEC mechanism. Hydrophobic stationary phases, such as C18, … solvent modifier (THF) in the mobile phase to reduce non-SEC interactions of the solute with the stationary phase. …

Organic Solvent Modifier and Temperature Effects in Non-Aqueous Size-Exclusion Chromatography on Reversed-Phase Columns

January 5, 2018
Author(s)
Andre M. Striegel, Anna Caltabiano, Joe Foley
… an inert surface suitable for the analysis of polymers by SEC. The bonded phases show remarkable differences in size … elution volumes of polymers with the temperature increase. SEC partition coefficients are temperature independent in the … elution of PS and PMMA polymers is by a near-ideal SEC on reversed-phase columns. Non-ideal SEC is possible for …

Effect of Tacticity on the Structure of Poly (1-Octadecene)

September 1, 2004
Author(s)
David L. VanderHart, E Perez, A Bello, G B. Vasquez, Manuel A. Quijada
… and the original polymer have been characterized by SEC, solution and solid-state 13C-NMR, DSC and X-ray … crystalline structure, DSC, NMR, poly(1-octadecene), SEC, tacticity, Xray …

SEC-SAXS/MC Ensemble Structural Studies of the Microtubule Binding Protein Cdt1 Show Monomeric, Folded-Over Conformations

June 1, 2025
Author(s)
Kyle Smith, Srinivas Chakravarthy, Manas Chakraborty, Kristen M. Vosberg, Marco Tonelli, Maximilian G. Plach, Arabela A. Grigorescu, Joseph E. Curtis, Dileep Varma
… DLS shows that Cdt1 is monomeric and polydisperse, while SEC-MALS confirms that it is monomeric at high … but without any apparent inter-molecular self-association. SEC-SAXS enabled computational modeling of the protein … SEC-SAXS/MC Ensemble Structural Studies of the Microtubule …

Size-exclusion chromatography

April 20, 2023
Author(s)
Andre Striegel
… Over the past half-century, size-exclusion chromatography (SEC) has become the premier method by which to determine the … When coupled to a multiplicity of detection methods, SEC also has the ability to inform our knowledge of the … an introduction and overview to the fundamental aspects of SEC, as appertain chromatographic retention, band broadening, …

On the feasibility of Raman spectroscopy as a continuous off-line detection method for size- exclusion chromatography and related techniques

July 1, 2015
Author(s)
Leena M. Pitkanen, Aaron A. Urbas, Andre Striegel
… as a detection method in size- exclusion chromatography (SEC) and related macromolecular separations, for the purposes … To this effect, heart-cutting fractions from the SEC peak of a gradient random copolymer of styrene (S) and … resembling those of eluting slices in an analytical SEC experiment,and subsequently analyzed by off-line Raman. …

Determining the solution conformational entropy of oligosaccharides by size-exclusion chromatography with on-line viscometry detection

March 12, 2014
Author(s)
Mallory J. Morris, Andre Striegel
… on the on-line coupling of size-exclusion chromatography (SEC), an entropically-controlled separation technique, and differential viscometry (VISC). Results from this SEC/VISC method were compared, for the same injections of the … conditions, to results from a benchmark SEC/differential refractometry (SEC/DRI) method which has …

Size-exclusion chromatography with multi-angle static light scattering

June 26, 2025
Author(s)
Andre Striegel, Amandaa Brewer, Claudia Zielke
… coupled with multi-angle static light scattering (SEC/MALS) is a combined separation and detection technique … set-up, sample preparation and controls needed for SEC/MALS experiments; how data should be processed; the size … results. We examine some of the applications of SEC/MALS, including the determination of molar mass averages …

Size Separation of Single Wall Carbon Nanotubes by Flow-Field Flow Fractionation

April 1, 2008
Author(s)
Jae H. Chun, Jeffrey A. Fagan, Erik K. Hobbie, Barry J. Bauer
… fractions made by use of size exclusion chromatography (SEC). The SEC fractions are well resolved by Flow-FFF. SWNT … compared to calibrations of hydrodynamic volume from the SEC fractions derived from dissolved polymers. Rod lengths of …

Size-Exclusion Chromatography: A twenty-first Century Perspective

April 4, 2022
Author(s)
Andre Striegel
… Now in its sixth decade, size-exclusion chromatography (SEC) remains the premier method by which to determine the … dimension and its change as a function of molar mass; etc. SEC is also an integral part of most macromolecular … mass distributions of a sample. While the potential of SEC remains strong, our awareness of the pitfalls and …

Chromatographic Fractionation of SWNT/DNA Dispersions With On-Line Multi Angle Light Scattering

November 6, 2008
Author(s)
Barry J. Bauer, Jeffrey A. Fagan, Erik K. Hobbie, Jae H. Chun, Vardhan Bajpai
… separated by size using a size exclusion chromatographic (SEC) column. On-line measurements are made of SWNT … this data. The SWNTs are shown to separate primarily by a SEC mechanism, but the elution times are effected by a high … of small quantities of large, polydisperse SWNTs after the SEC column limit. MALS data is fit with a polydisperse …

Size-exclusion chromatography of metal nanoparticles and quantum dots

April 8, 2016
Author(s)
Leena M. Pitkanen, Andre Striegel
… technique such as size-exclusion chromatography (SEC) is often needed to obtain narrow distribution NP … optical properties of NPs have been obtained by coupling SEC to detection methods such as ultraviolet-visible and/or … associated with the sorption of NPs and QDs onto various SEC stationary phases, employing both aqueous and organic …

Development of Aqueous Size Exclusion Chromatography Conditions to Characterize Polyzwitterion-block-N-isopropyl acrylamide Copolymers

July 21, 2020
Author(s)
Phillip Pickett, Sara Orski, Vivek Prabhu
… are a few examples of using size exclusion chromatography (SEC) for characterization, in which hexafluoro isopropanol … and hazardous HFIP is. Therefore, alternatives to HFIP for SEC analysis would be desirable. Here, a systematic methodology is demonstrated to apply aqueous SEC using several solvent conditions to enable the elution of …

Size-Exclusion Chromatography of Asphaltenes: An Experimental Comparison of Commonly-Used Approaches

May 4, 2013
Author(s)
Shen Dong, Andre Striegel
… range of these materials. Size-exclusion chromatography (SEC) is among the most widely employed methods for … seen widespread application in the study of asphaltenes. SEC analysis of asphaltenes presents a number of challenges, … compared several of the most commonly used approaches to SEC of asphaltenes, in hope of both demonstrating the …

Measurement of Single Wall Nanotube Dispersion by Size Exclusion Chromatography

May 9, 2007
Author(s)
Barry J. Bauer, Matthew Becker, Vardhan Bajpai, Jeffrey A. Fagan, Erik K. Hobbie, Kalman D. Migler, Charles M. Guttman, William R. Blair
… into isolated nanotubes. Size exclusion chromatography (SEC) is shown to be an effective method to characterize … single wall carbon nanotube (SWNT) dispersions. SEC separates nanotube dispersions by size, and measures the … microscopy (AFM) produced results consistent with the SEC method. …

Vibration of a Room-Sized Airspring-Supported Slab

January 1, 1998
Author(s)
H Amick, B Sennewald, N C. Pardue, E C. Teague, Brian R. Scace
… achieve one-third octave band amplitudes as low as 0.25 ?m/sec (10 ?in/sec) over some frequency ranges. …
Displaying 1 - 25 of 136
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