Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Size-exclusion chromatography

Published

Author(s)

Andre Striegel

Abstract

Over the past half-century, size-exclusion chromatography (SEC) has become the premier method by which to determine the molar mass (M) averages and the molar mass distribution (MMD) of natural and synthetic macromolecules. When coupled to a multiplicity of detection methods, SEC also has the ability to inform our knowledge of the physicochemical properties of polymers. This chapter provides an introduction and overview to the fundamental aspects of SEC, as appertain chromatographic retention, band broadening, and resolution. Also covered are the determination of "absolute" (i.e., calibrant-independent) M averages and MMDs by on-line coupling of SEC to viscometric and/or static light scattering detectors, and the role of SEC in two-dimensional macromolecular separations.
Citation
Liquid chromatography: Fundamentals and Instrumentation, 3rd edition
Volume
1
Publisher Info
Elsevier, Amsterdam,

Keywords

Size-exclusion chromatography, fundamentals, historical background, retention, band broadening, resolution, multiple detection, light scattering, viscometry, 2D separations

Citation

Striegel, A. (2023), Size-exclusion chromatography, Liquid chromatography: Fundamentals and Instrumentation, 3rd edition, Elsevier, Amsterdam, , [online], https://doi.org/10.1016/B978-0-323-99968-7.00022-9, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=933552 (Accessed May 20, 2024)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created April 20, 2023, Updated June 7, 2023