Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Chromatographic Fractionation of SWNT/DNA Dispersions With On-Line Multi Angle Light Scattering



Barry J. Bauer, Jeffrey A. Fagan, Erik K. Hobbie, Jae H. Chun, Vardhan Bajpai


Single wall carbon nanotubes (SWNTs) dispersed in aqueous medium by the use of DNA are separated by size using a size exclusion chromatographic (SEC) column. On-line measurements are made of SWNT concentration, molar mass, and size using UV-Vis absorption detection and multi-angle light scattering (MALS). Molar mass and length distributions are calculated for SWNT dispersions from this data. The SWNTs are shown to separate primarily by a SEC mechanism, but the elution times are effected by a high performance liquid chromatography (HPLC) mechanism in which adsorption on the column delays the elution of the components.This is most evident by the elution of small quantities of large, polydisperse SWNTs after the SEC column limit. MALS data is fit with a polydisperse anisotropic rod scattering function giving low polydispersity for most of the fractions with higher polydispersities near the large size exclusion limit and beyond the small size exclusion limit of the SEC column. These fits indicate that molar mass is proportional to the rod length over a wide range indicating rigid-rod behavior.
Journal of Physical Chemistry C


carbon nanotube, chromatography, fractionate, length, light scattering, rod, SWNT


Bauer, B. , Fagan, J. , Hobbie, E. , Chun, J. and Bajpai, V. (2008), Chromatographic Fractionation of SWNT/DNA Dispersions With On-Line Multi Angle Light Scattering, Journal of Physical Chemistry C, [online], (Accessed June 20, 2024)


If you have any questions about this publication or are having problems accessing it, please contact

Created November 6, 2008, Updated February 19, 2017