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Bioscience

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Displaying 126 - 150 of 1242

Mass Spectral Library Methods for Analysis of Site-Specific N-Glycosylation: Application to Human Milk Proteins

September 16, 2022
Author(s)
Concepcion Remoroza, Meghan Burke Harris, Xiaoyu (Sara) Yang, Sergey Sheetlin, Yuri Mirokhin, Sanford Markey, Dmitrii V. Tchekhovskoi, Stephen E. Stein
We present a mass-spectral library-based method for analyzing site-specific N-linked protein glycosylation. Its operation and utility are illustrated by applying it to both newly measured and available proteomics data of human milk glycoproteins. It

Report on the DHS/NIST Workshop on Standards for an Enduring Capability in Wastewater Surveillance for Public Health (SWWS Workshop)

August 16, 2022
Author(s)
Nancy Lin, Stephanie Servetas, Scott Jackson, Katrice Lippa, Kirsten Parratt, Philip Mattson, Clare Beahn, Mia Mattioli, Sally Gutierrez, Michael Focazio, Ted Smith, Paul Storella, Sarah Wright
Wastewater surveillance is a promising approach to monitor biological and chemical contaminants on a community level in support of public health and safety decisions. Wastewater surveillance has recently been shown highly effective for the early detection

Structural Fingerprinting of siRNA Therapeutics by Solution NMR Spectroscopy

August 4, 2022
Author(s)
Owen Becette, Anh Tran, Jace Jones, John Marino, Robert Brinson
Nucleic acids are an increasingly popular platform for the development of biotherapeutics to treat a wide variety of illnesses, including diseases where traditional drug development efforts have failed. To date, there are 14 short oligonucleotide

Effect of Tribologically-Induced Changes in Surface Termination of Silicon-Containing Diamond-Like Carbon Coatings on the Resistance to Biomolecule Adsorption

August 1, 2022
Author(s)
Cherno Jaye, Daniel A. Fischer, Zixuan Li, Jennings Z. Ye, Jieming Yan, Nicolas Molina, Hsu-Ming Lien, Robert Chrostowksi, Jianliang Lin, Mangolini Mangolini
Silicon-containing diamond-like carbon (DLC) is a class of thin-film materials with excellent mechanical properties, high thermal stability, and good tribological performance over a wide range of environmental conditions. While non-alloyed/non-doped DLCs

NMR and Metabolomics -A Roadmap for the Future

July 23, 2022
Author(s)
Tracey Johnston, David Wishart, Leo Cheng, Valerie Copie, Arthur Edison, Hamid Eghbalnia, Jeffrey Hoch, Goncalo Gouveia, Wimal Pathmasiri, Robert Powers, Llyod Sumner, Mario Uchimiya
Metabolomics investigates global metabolic alterations associated with chemical, biological, physiological, or pathological processes. These metabolic changes are measured with various analytical platforms. While liquid chromatography-mass spectrometry (LC

Selective C-terminal Conjugation of Trypsin-Digested Peptides for Proteomic Measurements

July 20, 2022
Author(s)
Tian Xie, Alexandria Brady, Cecilia Velarde, David Vaccarello, Nicholas Callahan, John Marino, Sara Orski
Bottom-up proteomic experiments often require selective conjugation or labeling of the N- and/or C-termini of peptides either through bottom up approaches or selective post-cleavage functionalization of specific residues after proteolytic digestion . For

The need for multicomponent gas standards for breath biomarker analysis

July 7, 2022
Author(s)
Kavita Jeerage, Jennifer Berry, Jacolin Murray, Cassie Goodman, Paulina Piotrowski, Christina Jones, Christina Cecelski, Jennifer Carney, Katrice Lippa, Tara Lovestead
Exhaled breath is a non-invasive, information-rich matrix with the potential to diagnose or monitor disease, including infectious disease. Despite significant effort dedicated to biomarker identification in case control studies, very few breath tests are

Computational Study of the Allosteric Effects of p5 on the CDK5-p25 Hyperactivity as Alternative Inhibitory Mechanisms in Neurodegeneration

June 30, 2022
Author(s)
Tejaswi Tammareddy, Walid Keyrouz, Ram D. Sriram, Harish C. Pant, Antonio Cardone, Jeffery B. Klauda
The cyclin-dependent kinase (CDK5) forms a stable complex with its activator p25, leading to the hyperphosphorylation of tau proteins and to the formation of plaques and tangles that are considered to be one of the typical causes of Alzheimer's disease (AD

Evidence that gecko setae are coated with an ordered nanometer-thin lipid film

June 9, 2022
Author(s)
Cherno Jaye, Daniel A. Fischer, Mette Rasmussen, Katinka Holler, Joe Baio, Stanislav Gorb, Tobias Weidner
Motivated by the fascination of gecko adhesion based on the fibrous setae at the tips of their toe pads and their potential for biomimetic applications, the adhesion mechanism has been studied in detail. While the mechanism has been shown to be based on

Reference Material 8376 Microbial Pathogen DNA Standards for Detection and Identification

June 1, 2022
Author(s)
Jason Kralj, Dieter Tourlousse, Monique Hunter, Erica Romsos, Blaza Toman, Peter Vallone, Scott Jackson
Reference Material (RM) 8376 is intended for NGS-based measurements quantitative to the chromosome. A unit of RM 8376 consists of 20 components (A-T, 19 bacteria and 1 human) each containing well-characterized DNA in 10 mmol/L Tris-HCl, 1 mmol/L EDTA pH 8

Benchmarking challenging small variants with linked and long reads

May 11, 2022
Author(s)
Justin Wagner, Nathanael Olson, Lindsay Harris, Marc L. Salit, Fritz Sedlazeck, Chunlin Xiao, Justin Zook
Genome in a Bottle benchmarks are widely used to help validate clinical sequencing pipelines and develop variant calling and sequencing methods. Here we use accurate linked and long reads to expand benchmarks in 7 samples to include difficult-to-map

Reference Materials for MS-based Untargeted Metabolomics and Lipidomics: A Review by the Metabolomics Quality Assurance and Quality Control Consortium (mQACC)

April 9, 2022
Author(s)
Katrice Lippa, William Davis, Christina Jones, Tracey Johnston, Juan Aristizabal-Henao, Richard Beger, John Bowden, Corey Broeckling, Chris Beecher, Warwick Dunn, Roberto Flores, Goncalo Gouveia, Thomas Hartung, Matthew Lewis, Ioanna Ntai, Andrew Percy, Dan Raftery, Jinchun Sun, Georgios Theodoridis, Fariba Tayyari, Federico Torta, Candice Ulmer, Baljit Ubhi
Introduction The metabolomics quality assurance and quality control consortium (mQACC) is enabling the identification, development, prioritization and promotion of suitable reference materials (RMs) to be used in quality assurance (QA) and quality control

A complete reference genome improves analysis of human genetic variation

April 1, 2022
Author(s)
Sergey Aganezov, Stephanie Yan, Daniela Soto, Melanie Kirsche, Samantha Zarate, Justin Wagner, Jennifer McDaniel, Nathanael David Olson, Rajiv McCoy, Megan Dennis, Justin Zook, Michael Schatz
Compared to its predecessors, the Telomere-to-Telomere CHM13 genome adds nearly 200 million base pairs of sequence, corrects thousands of structural errors, and unlocks the most complex regions of the human genome for clinical and functional study. We show

Complete genomic and epigenetic maps of human centromeres

April 1, 2022
Author(s)
Nicolas Altemose, Glennis Logsdon, Andrey Bzikadze, Pragya Sidhwani, Sasha Langley, Gina Caldas, Justin Zook, Ivan Alexandrov, Karen Miga
Existing human genome assemblies have almost entirely excluded repetitive sequences within and near centromeres, limiting our understanding of their organization, evolution, and functions, which include facilitating proper chromosome segregation. Now, a
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