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In-Situ Surface-Directed Assembly of 2D Metal Nanoplatelets for Drug-Free Treatment of Antibiotic-Resistant Bacteria



Parinaz Fathi, Ayman Roslend, Maha Alafeef, Parikshit Moitra, Ketan Dighe, Mandy Esch, Dipanjan Pan


The development of antibiotic resistance among bacterial strains is a major global public health concern. To address this, drug-free antibacterial approaches are needed. High-touch surfaces in particular can serve as a means for the spread of bacteria and other pathogens from one infected person to another. Copper surfaces have long been known for their antibacterial properties. In this work, we used a one-step surface modification technique to assemble 2D copper chloride nanoplatelets directly onto copper surfaces such as copper tape, transmission electron microscopy (TEM) grids, electrodes, and granules. The nanoplatelets were formed using copper ions from the copper surfaces, enabling their direct assembly onto these surfaces in a one-step process that does not require separate nanoparticle synthesis. The synthesis of the nanoplatelets was confirmed with TEM, scanning electron microscopy, energy dispersive spectroscopy (EDS), x-ray diffraction (XRD), and fourier transform infrared spectroscopy (FT-IR). Antibacterial properties of the Cu nanoplatelets were demonstrated in multi-drug-resistant (MDR) E. Coli. The Cu nanoplatelets led to a marked improvement in antibacterial properties compared to the copper surfaces alone. Cu nanoplatelets affected bacterial cell morphology, prevented bacterial cell division, reduced their viability, damaged bacterial DNA, and altered protein expression. In particular, proteins corresponding to cell division, DNA division, and mediation of copper toxicity were down-regulated. This work presents a robust method to directly assemble copper nanoplatelets onto any copper surface to imbue it with improved antibacterial properties. We additionally demonstrate the synthesis of other metal-derived nanoarchitectures on a variety of metal surfaces.
Advanced Healthcare Materials


Fathi, P. , Roslend, A. , Alafeef, M. , Moitra, P. , Dighe, K. , Esch, M. and Pan, D. (2022), In-Situ Surface-Directed Assembly of 2D Metal Nanoplatelets for Drug-Free Treatment of Antibiotic-Resistant Bacteria, Advanced Healthcare Materials, [online],, (Accessed June 25, 2024)


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Created July 20, 2022, Updated December 6, 2022