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Reference Material 8376 Microbial Pathogen DNA Standards for Detection and Identification



Jason Kralj, Dieter Tourlousse, Monique Hunter, Erica Romsos, Blaza Toman, Peter Vallone, Scott Jackson


Reference Material (RM) 8376 is intended for NGS-based measurements quantitative to the chromosome. A unit of RM 8376 consists of 20 components (A-T, 19 bacteria and 1 human) each containing well-characterized DNA in 10 mmol/L Tris-HCl, 1 mmol/L EDTA pH 8 buffer. Each component consists of one skirted 0.5-mL tube containing approximately 100 uL of DNA solution. Each tube is labeled and sealed with a removable screwcap. Here, we have documented the production, analytical methods, and statistical evaluation of realizing this RM.
Special Publication (NIST SP) - 260-225
Report Number


pathogen, microbial, DNA, metagenomics


Kralj, J. , Tourlousse, D. , Hunter, M. , Romsos, E. , Toman, B. , Vallone, P. and Jackson, S. (2022), Reference Material 8376 Microbial Pathogen DNA Standards for Detection and Identification, Special Publication (NIST SP), National Institute of Standards and Technology, Gaithersburg, MD, [online],, (Accessed May 18, 2024)


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Created June 1, 2022, Updated November 29, 2022