Reference Materials for MS-based Untargeted Metabolomics and Lipidomics: A Review by the Metabolomics Quality Assurance and Quality Control Consortium (mQACC)

Published

April 9, 2022

Author(s)

Katrice Lippa, William Davis, Christina Jones, Tracey Schock, Juan Aristizabal-Henao, Richard Beger, John Bowden, Corey Broeckling, Chris Beecher, Warwick Dunn, Roberto Flores, Goncalo Gouveia, Thomas Hartung, Matthew Lewis, Ioanna Ntai, Andrew Percy, Dan Raftery, Jinchun Sun, Georgios Theodoridis, Fariba Tayyari, Federico Torta, Candice Ulmer, Baljit Ubhi

Abstract

Introduction The metabolomics quality assurance and quality control consortium (mQACC) is enabling the identification, development, prioritization and promotion of suitable reference materials (RMs) to be used in quality assurance (QA) and quality control (QC) for untargeted metabolomics research. Objectives This review aims to highlight current RMs and methodologies used within untargeted metabolomics and lipidomics communities to ensure standardization of results obtained from data analysis, interpretation and cross-study and cross-laboratory comparisons. Results The potential for game-changing biochemical discoveries through mass spectrometry (MS) untargeted metabolomics and lipidomics are predicated on the evolution of more confident (and eventual quantitative) results from research laboratories. RMs are thus critical QC tools to be able to assure standardization, comparability and reproducibility for untargeted data analysis and interpretation and to compare data within and across studies and across multiple laboratories. Standard operating procedures that promote, describe and exemplify the use of RMs will also improve QC for the metabolomics and lipidomics communities. Conclusions The application of RMs described in this review may significantly improve data quality to support metabolomics and lipidomics research. The continued development and deployment of new RMs together with interlaboratory studies and educational outreach and training will further promote sound QA practices in the community.

Citation

Metabolomics

Volume

18

Pub Type

Journals

Download Paper

https://doi.org/10.1007/s11306-021-01848-6

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Keywords

reference materials, certified reference materials, untargeted analysis, mass spectrometry, metabolomics, lipidomics, metabolomics quality assurance and quality control consortium (mQACC)

Citation

Lippa, K. , Davis, W. , Jones, C. , Schock, T. , Aristizabal-Henao, J. , Beger, R. , Bowden, J. , Broeckling, C. , Beecher, C. , Dunn, W. , Flores, R. , Gouveia, G. , Hartung, T. , Lewis, M. , Ntai, I. , Percy, A. , Raftery, D. , Sun, J. , Theodoridis, G. , Tayyari, F. , Torta, F. , Ulmer, C. and Ubhi, B. (2022), Reference Materials for MS-based Untargeted Metabolomics and Lipidomics: A Review by the Metabolomics Quality Assurance and Quality Control Consortium (mQACC), Metabolomics, [online], https://doi.org/10.1007/s11306-021-01848-6, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=932182 (Accessed April 25, 2024)