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Bioscience

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Displaying 1051 - 1075 of 1242

Reference Materials for DNA Analysis

March 1, 2001
Author(s)
Barbara C. Levin, D J. Reeder
Mention has already been made, in Section 5.1. Of the use of DNA-based techniques for control of microbiological DNA. In parallel to the work of ATCC the USA National Institute of Standards and Technology (NIST) developed three Standard Reference Materials

The Development of Fluorescence Intensity Standards

March 1, 2001
Author(s)
Adolfas K. Gaigalas, L. Li, O. Henderson, R F. Vogt, J. Barr, G E. Marti, J. Weaver, A. Schwartz
The use of fluorescence as an analytical technique has been growing over the last 20 years. A major factor in inhibiting more rapid growth has been the inability to make comparable fluorescence intensity measurements across laboratories. NIST recognizes

Ab Initio Structure of the Active Site of Phosphotriesterase

February 1, 2001
Author(s)
Morris Krauss
This research exploits two recent developments to obtain a fundamental understanding of the metalloenzyme active site using the bi-metallic enzyme phosphotriesterase as an example of this class. First, is the theoretical prediction that the structure and

CASSCF Investigation of Electronic Excited States of 2-Aminopurine

January 11, 2001
Author(s)
E L. Rachofsky, J B. Ross, Morris Krauss, R Osman
2-aminopurine is a highly fluorescent analog of adenine that can be incorporated synthetically into DNA with little perturbation of the native double-helical structure. The sensitive dependence of the quantum yield of this fluorophore on nucleic acid

A new approach to assess mAb aggregation

January 1, 2001
Author(s)
Illarion Turko
A proof-of-concept for new methodology to detect and potentially quantify mAb aggregation is presented. Assay development included using an aggregated mAb as bait for screening of a phage display peptide library and identifying those peptides with random

Gene Expression Analysis on Medium-Density Oligonucleotide Arrays

January 1, 2001
Author(s)
R. Sinibaldi, C D. O'Connell, H Rodriguez, C. Seidel
As the human genome project continues toward its goal of sequencing the entire human genome by the end of 2003, this is providing unique opportunities for studying genetic variation in humans and its relationship with disease risk and aging. Consequently

Estimation of the Hydrophobic Effect in an Antigen-Antibody Protein-Protein Interface

December 19, 2000
Author(s)
E. J. Sundberg, M. Urrutia, B C. Braden, J. Isern, D. Tsuchiya, B. A. Fields, E Malchiodi, J. Tormo, Frederick P. Schwarz, R A. Mariuzza
Antigen-antibody complexes provide useful models for analyzing the thermodynamics of protein-protein association reactions. We have employed site-directed mutagenesis, X-ray crystallography, and isothermal titration calorimetry to investigate the role of

Repair of oxidative DNA damage in Drosophila melanogaster: identification and characterization of dOgg1, a second DNA glycosylase activity for 8-hydroxyguanine and formamidopyrimidines

December 1, 2000
Author(s)
C. Dherin, M. Dizdaroglu, H. Doerflinger, S. Boiteux, J. P. Radicella
In Drosophila, the S3 ribosomal protein has been shown to act as a DNA glycosylase/AP lyase capable of releasing 8-hydroxyguanine (8-OH-Gua) in damaged DNA. Here we describe a second Drosophila protein (dOggl) with 8-OH-Gua and abasic (AP) site DNA repair

Infrared Spectroscopic Ellipsometry of Self-Assembled Monolayers

November 28, 2000
Author(s)
Curtis W. Meuse
Ellipsometry measures the relative intensity of and the phase difference between the parallel (p) and perpendicular (s) components of an electric field vector interacting with a sample. In this paper, a technique using polarized Fourier transform infrared

Stressing-Out DNA? The Contribution of Serine-Phosphodiester Interactions in Catalysis by Uracil DNA Glycosylase

October 17, 2000
Author(s)
R M. Werner, Y. L. Jiang, R. G. Gordley, J. Jagadeesh, Jane E. Ladner, G Xiao, M Tordova, G L. Gilliland, J T. Stivers
The DNA repair enzyme uracil DNA glycosylase (UDG) pinches the phosphodiester backbone of damaged DNA using the hydroxyl side chains of a conserved trio of serine residues, resulting in flipping of the deoxyuridine from the DNA helix into the enzyme active

Prokaryotic Expression, Purification, and Reconstitution of Biological Activities (Antiprotease, Antitumor and Heparin-Binding) for Tissue Factor Pathway Inhibitor- 2

October 5, 2000
Author(s)
C N. Rao, Prasad T. Reddy, D J. Reeder, Yonglin Liu, S M. Stack, W Kisiel, D T. Woodley
We report the expression of tissue factor pathway inhibitor-2 (TFPI-2) (also known as PP-5, placental protein-5; MSPI, matrix-associated serine protease inhibitor) in E. coli as a 25 kDa non-glycosylated protein with a glycine substituted for aspartic acid
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