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A new approach to assess mAb aggregation



Illarion Turko


A proof-of-concept for new methodology to detect and potentially quantify mAb aggregation is presented. Assay development included using an aggregated mAb as bait for screening of a phage display peptide library and identifying those peptides with random sequence which can recognize mAb aggregates. The selected peptides can be used for developing homogeneous quantitative methods to assess mAb aggregation. Results indicate that a peptide binding method coupled with fluorescence polarization detection can detect mAb aggregation and potentially monitor the propensity of therapeutic protein candidates to aggregate.
Methods in Molecular Biology


Monoclonal antibodies, Aggregation, Peptide phage display, Next generation sequencing, FITC-peptide


Turko, I. (2001), A new approach to assess mAb aggregation, Methods in Molecular Biology (Accessed April 25, 2024)
Created January 1, 2001, Updated October 11, 2022