Projects/Programs

Displaying 1 - 23 of 23

Cancer Genome in a Bottle

Ongoing

Goals: This project is an extension of the Genome in a Bottle Consortium to develop the technical infrastructure (reference standards, reference methods, and reference data) to enable translation of cancer genome sequencing to clinical practice and innovations in technologies. The priority of GIAB

Cell Viability for Advanced Therapies

Ongoing

In the Biosystems and Biomaterials Division at NIST, we have established several technical programs to support the development and implementation of cell viability assays in advanced therapies. These programs address key needs in cell viability assay development including selection and development

Clinical and Forensic Reference Materials

Ongoing

Below is a listing of the reference materials currently produced by the Applied Genetics group. Clinical Diagnostics SRM 2393 - CAG Repeat Length Mutation in Huntington`s Disease SRM 2365 - BK Virus DNA Quantitative Standard SRM 2366a - Cytomegalovirus DNA (Towne Δ147 BAC) for DNA SRM 2367 - JC

Map of the world with the engineering biology topics covered in Americas, Europe, and Asia meetings

Engineering Biology Metrics and Technical Standards for the Global Bioeconomy

Ongoing

Stakeholders from the Americas, Asia and Australia, and Europe and Africa, came together through a series of regional workshops in Washington D.C., Singapore, and Brussels, respectively, to identify specific areas for development, both technical and non-technical towards continued scale-up and economic growth across the bioeconomy.

FAQs - Genome in a Bottle

Ongoing

The inside of a DNA extraction robot hold tube in a rack

Forensic Genetics

Ongoing

Since the late 1980s, NIST has had scientists involved in DNA testing (aka "DNA Fingerprinting"). Early concerns over measurement accuracy and poor quality control of forensic DNA tests caused the Department of Justice to call upon NIST scientists to help with standards development and technology

H5 Influenza Positive Controls

Ongoing

In response to the current H5N1 avian influenza outbreak, NIST has developed a research grade test material (RGTM) for PCR-based assays. This material contains 3 vials with RNA fragments for HA (Part A), NA (Part B), and MP (Part C). A NIST Reference Material is also under development. The guidance

LANTERN - Interpretable modeling of genotype-phenotype landscapes with state-of-the-art predictive power

Ongoing

LANTERN: genotype-phenotype LANdscape in TERpretable Non-parametric model The bioeconomy is increasingly data-driven, relying on large-scale measurements to rapidly engineer novel functions. To facilitate rational engineering, where complex designs are constructed from well understood components

Living Yeast Cell Reference Material (RM 8230)

Ongoing

Quantification of total and viable microbial cells is critical for applications ranging from assessment of DNA extraction efficiency in support of microbial metagenomics to characterization of antimicrobial efficacy. In addition, safe biological materials are needed to substitute for true biothreat

Methods to determine the evolutionary stability of engineered biological function

Ongoing

Advances in engineering biology have greatly expanded our ability to add new and useful functions to living cells. Engineering stable functions has remained a significant challenge, with many different approaches developed to increase the stability, or decrease the variability, of a function over

Gears illustrating NIST's cell-free systems initiatives

Metrology For Cell-Free Expression Systems

Ongoing

Cell-free expression systems are poised to advance the US bioeconomy and play a key role in ensuring US manufacturing resilience. NIST has several, on-going projects in its growing portfolio to promote reproducibility, new measurement methods, and best practices and standards in cell-free expression

Graphic of a erlenmeyer flask containing tilled soil, a green leaf, and yellow wheat.

Molecular Innovation in Agriculture

Ongoing

Plant science and breeding aim to develop new plant phenotypes with desired qualities, increased production, disease resistance, and traits that reduce water usage. At the federal level, plant research is conducted by the USDA's Animal and Plant Health Inspection Service (APHIS) and Agricultural

NIST/FDA CVM Evaluation of Assays and Control Materials for Characterizing Genome-Edited Animal Biotechnology Products

Ongoing

FDA CVM oversees regulation of IGAs in animals, including those that have been created using genome editing technologies. Characterization of these IGAs is an important part of the regulatory process to ensure that the edit to the animal’s genome is what it is intended to be and to identify any

NIST Flow Cytometry Standards Consortium 

Ongoing

Advances in cell and gene-based therapeutics as well as other regenerative medicine products have increased the need for high quality, robust, and validated measurements for cell characterization. Flow cytometry, including imaging cytometry, has emerged as an important platform due to its ability to

NIST LabCAS: Data Driven Science Architecture and Resource

Ongoing

Biological data lie at the heart of innovation and artificial intelligence (AI) advances in the emerging biotechnology sector and serve as key strategic resources for achieving major breakthroughs in biomanufacturing, as summarized by the National Security Commission on Emerging Biotechnology's

NIST Living Measurement Systems Foundry

Ongoing

To enable the production of high-throughput, high-quality data that meets the rapidly evolving needs of current and future stakeholders, NIST has established an automation facility for the growth, manipulation, sample preparation, and measurement of engineered microbes. The core of the facility

NISTCHO

Ongoing

NISTCHO, a new mammalian cell line of interest to the biopharmaceutical and biomanufacturing communities, is now available from the National Institute of Standards and Technology. This Chinese hamster ovary (CHO) cell line has been engineered by MilliporeSigma to express a non-originator version of

RMAT Laboratory Programs

Ongoing

Examples of Current Activities: Cell Counting and viability are key measurements critical for decision making from R&D to manufacturing. We recently developed an approach for evaluating the quality of cell counting measurements through experimental design and statistical analysis that does not

Serology and Neutralization Assays for COVID-19

Ongoing

Multiplexed bead-based SARS-CoV-2 serological assay We have developed a validated multiplexed bead-based SARS-CoV-2 serological assay that measures different antibodies produced by a patient (IgG, IgM, IgA). In collaboration with the NIST Applied and Computational Mathematics Division, we have

Standards to Support Biological Detection in the Field

Ongoing

NIST is working in partnership with the Department of Homeland Security Science and Technology Directorate (DHS S&T) to develop methods, guidance, standards, and reference materials to support and improve our Nation's ability to detect and respond to suspected biothreat incidents. Specific

First responders collecting samples of the yeast surrogate material during a field exercise. (Credit: NIST)

Standards to Support Biothreat Detection

Ongoing

Since 9/11 and the subsequent anthrax attacks in 2001, suspicious white powder incidents continue to occur within the US, often requiring Federal law enforcement response. Suspect samples are sent to public health laboratories for confirmatory evaluation, which can take hours to weeks to receive

Surrogate Materials to Support Training in Biological Sampling and Detection in the Field

Ongoing

Nationwide, first responders regularly encounter suspicious materials that might be biological threat agents. Routine training and process evaluations help ensure workflows, operators, and technologies are working together properly within the concept of operations. Biological test materials have the

Synthetic Biology

Ongoing

New measurement methods and tools are required for biology to emerge fully as an enabling, practical platform for engineering. The Cellular Engineering Group works to provide a foundation of measurement assurance to support the control and rational design of biological function. Through state of the

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