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Bioscience

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Synthetic Fecal Metabolite Calibrant Solution (RGTM 10212)

Ongoing

Metabolomics is an exciting and dynamic field in life sciences that harnesses cutting-edge technologies to identify and characterize small molecules, or metabolites, within cells, tissues, or organisms, collectively known as the metabolome. The wealth of evidence in the literature underscores the

U.S. TAG for ISO/TC276: Biotechnology

Ongoing

Get involved: Interested U.S. Stakeholders may participate in the US TAG at no cost. Membership provides access to all ISO/TC 276 and US TAG documents, including standards under development. Please click here to join. Contribute to standards development: Please click here to review, submit comment

Tandem Mass Spectral Library

Ongoing

Intended Impact Modern mass spectrometers commonly generate ions by electrospray mass spectrometry which are separated and then fragmented by collisional dissociation. This process yields fragment ion ‘fingerprints’ (or mass spectra) that are widely used to identify the individual components in a

Targeting Membrane Proteins

Ongoing

Membrane proteins constitute approximately 30% of all cellular proteins. Nevertheless, this group of proteins is traditionally understudied due to limitations of the available analytical tools. Given the importance of membrane proteins in the various cellular processes and their potential as drug

Thermodynamic Principles Describe and Predict Cell Populations

Ongoing

Heterogeneity of cell populations indicates the many ways cells can process information. We study heterogeneity in gene expression by inserting a gene for a fluorescent protein downstream of the gene we are interested in, and we examine large numbers of cells by imaging or flow cytometry. We think

Thermodynamics of Enzyme-catalyzed Reactions

Ongoing

Current efforts are focused on industrially important biotransformation problems such as those found in hydrogenation, hydroxylation, aromatic amino acid metabolic pathways, and biofuel applications. The methods used include calorimetry, chromatography, enzymology, and spectrophotometry. The

Tissue Engineering

Ongoing

Tissue Engineering Measurands: Tissue engineering measurands are confusing to discuss and a charting method to improve communication is demonstrated. Measurement Assurance Strategies for Regenerative Medicine: Reliable methods for assessing product consistency and quality. Regenerative Medicine

Tissue Engineering Measurands

Ongoing

Making a Measurand Chart: A measurand chart can be created for any measurement by writing down all the measurands that occur during the measurement. The panels should not be procedural steps, such as “wash the sample with buffer” or “centrifuge the microfuge tubes”. Consider if the measurement has a

Traceability in Molecular Spectrophotometry

Ongoing

This program, sometimes referred to as the Optical Filters program, has supported the development, certification, and (as specified) recertification of Standard Reference Materials ® (SRMs ®) for the verification of the transmittance (absorbance) and wavelength scales of spectrophotometers in the

Trusted AI framework for a new class of Standard Reference Materials and Data with exquisitely characterized uncertainty for billions of properties

Ongoing

The measurement of diverse molecular classes in biospecimens containing millions of molecules, known as omics, is increasingly important in the life sciences and medicine, and used to find novel cancer therapeutics, discover COVID-19 infection pathways, and diagnose diseases. NIST RMs and SRDs for

Series of MRI images labeled A to E showing different views of brain tissue, some with gadolinium highlighted in blue against a purple background.

Understanding the Bioaccumulation of Gadolinium-Based Contrast Agents using Advanced Measurement Capabilities

Ongoing

Researchers and staff from the Medical University of South Carolina are collaborating with NIST staff experienced in trace elemental speciation, sample preparation and instrumental analysis in order to demonstrate the bioaccumulation of intact GBCA in patients who have repeated GBCA exposure

Illustration of three models with low, medium and high granularity.

Understanding Protein Solution Phase Behavior via Coarse-Grained Modeling

Ongoing

Intended Impact Because biological systems span a broad range of length and time scales, they cannot be studied effectively using conventional all-atom simulations. While hardware advances represent one route to improving the computational tractability of simulating biological systems, significant

Unfolding and Aggregation of Therapeutic Proteins

Completed

A stable protein formulation is essential to the safety of mAb drugs. In particular, mAb drug solutions formulated at high concentrations can undergo various biophysical instabilities, such as aggregation and liquid-liquid phase separation. These undesired solution properties are primarily driven by

Wastewater Surveillance

Ongoing

NIST is partnering with the Department of Homeland Security Science and Technology Directorate (DHS/S&T) to develop documentary standards and reference materials to support an enduring capability in wastewater surveillance. Ongoing activities include: Development of a research grade test material

WG1 Terminology

Ongoing

Standard(s) Under Development: ISO/WD TR 20386 Inventory of biotechnology-related terms US TAG WG1 Expert: Sumona Sarkar TAG Administrator: Clare Allocca

WG2 Biobanks and Bioresources

Ongoing

Standard(s) Under Development: ISO/AWI 20387 Biotechnology - Biobanking - General requirements for Biobanking US TAG WG2 Convenor: Nilsa Ramirez, MD, College of American Pathologists TAG Administrator: Clare Allocca

WG3 Analytical Methods

Ongoing

Standard(s) Under Development ISO/WD 20391-1: Biotechnology - Cell Counting – Part 1. General Guidance on Cell Counting Methods ISO/WD 20391-2: Biotechnology - Cell Counting – Part 2. Experimental Design and Statistical Analysis to Quantify Counting Method P erformance ISO/AWI 20395: Quality

WG4 Bioprocessing

Ongoing

Standard(s) Under Development: ISO/AWI TS 20399-1: Raw materials control for bioprocessing -- Part 1: General definitions and requirements for quality control ISO/AWI TS 20399-2: Raw materials control for bioprocessing -- Part 2: Best practice guide for supplies ISO/AWI TS 20399-3: Raw materials

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