To develop the thermodynamic data and methodology required for effective bioprocess engineering.
Current efforts are focused on industrially important biotransformation problems such as those found in hydrogenation, hydroxylation, aromatic amino acid metabolic pathways, and biofuel applications. The methods used include calorimetry, chromatography, enzymology, and spectrophotometry. The quantities that are measured, apparent equilibrium constants K' and calorimetrically determined enthalpies of reaction ΔrH(cal), are used to calculate values of standard equilibrium constants K and enthalpies of reaction ΔrH°. These values, in turn, can be used in an equilibrium model (http://xpdb.nist.gov/enzyme_thermodynamics/download.html), to predict the position of equilibrium and extent of reaction for a biochemical reaction as a function of temperature, pH, pMg, and ionic strength.