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Search Publications by: Laurie E. Locascio (Fed)

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Displaying 1 - 25 of 37

Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays

October 12, 2021
Gloria Thomas, Emad E. El-Giar, Laurie E. Locascio, Michael J. Tarlov
The integration of immunoassays in microfluidic devices is a rapidly developing research area combining the power of immunoassays with the inherent benefits of microfluidics. Here, a general overview of microfluidic-based immunoassays is presented along

Reagentless Cleavage of the Peptide Backbone Using Light-Generated Radicals

October 12, 2021
Barbara J. Jones, Mathew J. Vergne, David M. Bunk, Laurie E. Locascio, M A. Hayes
A key step for protein identification and characterization is cleavage into distinct fragments. All current cleavage methods require the addition of reagents, either proteolytic enzymes or chemical agents, and often a second reagent to discontinue cleavage


October 3, 2011
Samuel Stavis, Jon Geist, Michael Gaitan, Laurie E. Locascio, Elizabeth Strychalski
Entropophoresis - motion caused by an entropy gradient - is a novel nanofluidic method to direct the self-transport of biopolymers that established a new paradigm of nanofluidic functionality with broad relevance to lab-on-a-chip technol-ogy. Here, the

Quantum Dot FRET Sensor for Enzymatic Activity in Microfluidic Device

June 22, 2010
Georgeta Crivat, Sandra M. Da Silva, Darwin Reyes-Hernandez, Samuel Forry, Laurie E. Locascio, Michael Gaitan, Nitsa Rosenzweig, Zeev Rosenzweig
This paper describes the development of new fluorescence resonance energy transfer (FRET)-based quantum dot probes for proteolytic activity. The CdSe/ZnS quantum dots are incorporated into a thin polymeric film, which is prepared by layer-by-layer

Amphiphilic Block Copolymer Nanotubes and Vesicles Stabilized byPhotopolymerization

October 16, 2008
Ana Jofre, John B. Hutchison, Laurie E. Locascio, Kristian Helmerson
Photopolymerization is implemented to crosslink and stabilize vesicles and nanotubes composed of amphiphilic block copolymer chains. Stabilized membranes of nanotubes and vesicles were formed by the directed self-assembly of poly(ethylene oxide)-block

DHS Leads the Way to Standards for Homeland Security

October 16, 2008
Laurie E. Locascio
In late April 2005, in response to a recently issued GAO report describing the urgent need for standards and validated methods related to biological incident response, Dr. Bert Coursey, head of the Standards Portfolio at DHS, charged NIST with the task of

Electrokinetic Sample Dilution and Concentration in Microfluidics

October 16, 2008
David J. Ross, K G. Olsen, Laurie E. Locascio
A new method is demonstrated which can be used to produce streams of variable, controlled analyte concentration. The method works by electrokinetically manipulating the analyte velocity so that it is different in different parts of a channel. Because of

Microfluidic DNA Analysis Systems for Forensic Applications

July 18, 2008
Michael Gaitan, Jayna J. Shah, Darwin Reyes-Hernandez, Pierre-Alain Auroux, Jon Geist, Laurie E. Locascio, Wyatt N. Vreeland, David J. Ross, Peter Vallone, Paul Smith, Nicole Morgan, Tom Pohida, John Kakareka, Annelise Barron
This report summarizes the NIST effort on microfluidic DNA analysis systems for forensic applications sponsored by the National Institute of Justice. Currently emerging microfluidics-based forensic systems are implemented in silica (glass) because the

Liposome-Templated Supramolecular Assembly of Responsive Alginate Nanogels

March 14, 2008
Jennifer S. Hong, Wyatt N. Vreeland, Silvia H. De Paoli Lacerda, Laurie E. Locascio, Michael Gaitan, Srinivasa R. Raghavan
Nanosized gel particles (nanogels) are of interest for a variety of applications, including controlled release of drugs and single-molecule encapsulation. Here, we employ the cores of nanoscale liposomes as reaction vessels to template the assembly of

Preparation of Nanoparticles by Continuous-Flow Microfluidics

February 15, 2008
Andreas Jahn, Joseph E. Reiner, Wyatt N. Vreeland, Don DeVoe, Laurie E. Locascio, Michael Gaitan
We review a variety of micro- and nanoparticle formulations produced with microfluidic methods. A diverse variety of approaches to generate micro-scale and nano-scale particles have been reported. Here we emphasize the use of microfluidics, specifically

Liposome Characterization with Fluorescence Cumulant Analysis

June 8, 2007
Joseph E. Reiner, Andreas Jahn, Laurie E. Locascio, Michael Gaitan, John J. Kasianowicz
Liposomes are self-assembled spherical vesicles comprised of a lipid bilayer membrane that segregates an internal aqueous environment from an external aqueous environment. These nanometer-scale structures have demonstrated potential for targeted drug

Microfluidic Directed Formation of Liposomes of Controlled Size

April 24, 2007
Andreas Jahn, Wyatt N. Vreeland, Don DeVoe, Laurie E. Locascio, Michael Gaitan
A new method to tailor liposome size and size distribution in a microfluidic format is presented. Liposomes are spherical structures formed from lipid bilayers that are from tens of nanometers to several micrometers in diameter. Liposome size and size

Microfluidic Method for Thermal Cycling by Microwave Dielectric Heating

November 9, 2006
Pierre-Alain Auroux, Jayna J. Shah, James Booth, Mulpuri Rao, Laurie E. Locascio, Michael Gaitan
We report on temperature time domain and spectral measurements for on-chip microwave thermocycling. In particular we observe enhanced specificity of the heating of a targeted sample compared to conventional heating block-based thermocyclers. Spatial

Photopatterned Polyelectrolyte Multilayers for Cell Culture

November 9, 2006
Darwin Reyes-Hernandez, J. Brian Hutchison, Laurie E. Locascio, Michael Gaitan
Patterned two-dimensional cell cultures are increasingly important for constructing bioanalytical tools for cell and molecular biology to address questions about interactions, signaling, migration, and differentiation, among others. This contribution

Polyelectrolyte Multilayers

January 23, 2006
Darwin Reyes-Hernandez, Laurie E. Locascio, Michael Gaitan
The process of self-assembly occurs naturally in living systems to form complex structures out of random events in an incredibly accurate way. Order out of disorder is achieved in a fashion that is analogous to a jigsaw puzzle where each piece fits

Liposomes As Model Cellular Systems

December 21, 2004
Laurie E. Locascio, Wyatt N. Vreeland, Andreas Jahn, Michael Gaitan
The discovery that phospholipids spontaneously form fluid-filled bubbles when exposed to aqueous solution was first made by Alec Bangham in the early 1960's while studying the effects of phospholipids on blood clotting in the Babraham Institute in England