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Liposome Characterization with Fluorescence Cumulant Analysis

Published

Author(s)

Joseph E. Reiner, Andreas Jahn, Laurie E. Locascio, Michael Gaitan, John J. Kasianowicz

Abstract

Liposomes are self-assembled spherical vesicles comprised of a lipid bilayer membrane that segregates an internal aqueous environment from an external aqueous environment. These nanometer-scale structures have demonstrated potential for targeted drug delivery applications. For liposomes to be useful in vivo, the liposome size and dosage of molecules contained within them needs to be controlled. We present here a fluorescence-based technique for characterizing the relative encapsulation efficiency, leakage rate, and shelf life of liposome formulations. We also estimate the average size of the liposomes from fluorescence correlation spectroscopy (FCS) measurements. These parameters may prove useful for optimizing the liposome formation process.
Proceedings Title
Proceedings of SPIE Fluctuations and Noise in Biological, Biophysical, and Biomedical Systems
Conference Dates
May 20-24, 2007
Conference Location
Florence, 1, IT

Keywords

drug delivery, fluorescence correlation spectroscopy, fluorescence cumulant analysis, liposomes, microfluidics

Citation

Reiner, J. , Jahn, A. , Locascio, L. , Gaitan, M. and Kasianowicz, J. (2007), Liposome Characterization with Fluorescence Cumulant Analysis, Proceedings of SPIE Fluctuations and Noise in Biological, Biophysical, and Biomedical Systems, Florence, 1, IT, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=32666 (Accessed April 14, 2024)
Created June 7, 2007, Updated October 12, 2021