Liposome Characterization with Fluorescence Cumulant Analysis

Published

June 8, 2007

Author(s)

Joseph E. Reiner, Andreas Jahn, Laurie E. Locascio, Michael Gaitan, John J. Kasianowicz

Abstract

Liposomes are self-assembled spherical vesicles comprised of a lipid bilayer membrane that segregates an internal aqueous environment from an external aqueous environment. These nanometer-scale structures have demonstrated potential for targeted drug delivery applications. For liposomes to be useful in vivo, the liposome size and dosage of molecules contained within them needs to be controlled. We present here a fluorescence-based technique for characterizing the relative encapsulation efficiency, leakage rate, and shelf life of liposome formulations. We also estimate the average size of the liposomes from fluorescence correlation spectroscopy (FCS) measurements. These parameters may prove useful for optimizing the liposome formation process.

Proceedings Title

Proceedings of SPIE Fluctuations and Noise in Biological, Biophysical, and Biomedical Systems

Conference Dates

May 20-24, 2007

Conference Location

Florence, 1, IT

Pub Type

Conferences

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Keywords

drug delivery, fluorescence correlation spectroscopy, fluorescence cumulant analysis, liposomes, microfluidics

Citation

Reiner, J. , Jahn, A. , Locascio, L. , Gaitan, M. and Kasianowicz, J. (2007), Liposome Characterization with Fluorescence Cumulant Analysis, Proceedings of SPIE Fluctuations and Noise in Biological, Biophysical, and Biomedical Systems, Florence, 1, IT, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=32666 (Accessed April 16, 2024)