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Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays

Published

Author(s)

Gloria Thomas, Emad E. El-Giar, Laurie E. Locascio, Michael J. Tarlov

Abstract

The integration of immunoassays in microfluidic devices is a rapidly developing research area combining the power of immunoassays with the inherent benefits of microfluidics. Here, a general overview of microfluidic-based immunoassays is presented along with a method for immobilizing antibodies in polyacrylamide gel plugs set in microfluidic channels These antigen-specific hydrogels can be rapidly formed by photo-polymerizing monomer solutions mixed with antibodies, or other large proteins. The resulting antigen-specific hydrogels contain pore sizes appropriate for physical entrapment of large antibodies while remaining permeable to smaller proteins. The open structure of these hydrogels enables the capture and concentration of target antigens present at low concentrations. Such physical entrapment provides a conceptually simple method of immobilization compared to immobilization of proteins on surfaces, and offers advantages such as resistance to chemical and thermal denaturation.
Citation
Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays
Publisher Info
Methods in Molecular Biology Book Chapter, Methods in Molecular Medicine Book Series Format Guide by Humana Press,

Keywords

antibody immobilization, hydrogel, microdevice, microfluidic immunoassays, polyacrylamide gel plug

Citation

Thomas, G. , El-Giar, E. , Locascio, L. and Tarlov, M. (2021), Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays, Methods in Molecular Biology Book Chapter, Methods in Molecular Medicine Book Series Format Guide by Humana Press, (Accessed July 4, 2022)
Created October 12, 2021