Thomas, G.
, El-Giar, E.
, Locascio, L.
and Tarlov, M.
(2021),
Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays, Methods in Molecular Biology Book Chapter, Methods in Molecular Medicine Book Series Format Guide by Humana Press,
(Accessed October 15, 2024)
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays
Published
Author(s)
Gloria Thomas, Emad E. El-Giar, ,
Abstract
The integration of immunoassays in microfluidic devices is a rapidly developing research area combining the power of immunoassays with the inherent benefits of microfluidics. Here, a general overview of microfluidic-based immunoassays is presented along with a method for immobilizing antibodies in polyacrylamide gel plugs set in microfluidic channels These antigen-specific hydrogels can be rapidly formed by photo-polymerizing monomer solutions mixed with antibodies, or other large proteins. The resulting antigen-specific hydrogels contain pore sizes appropriate for physical entrapment of large antibodies while remaining permeable to smaller proteins. The open structure of these hydrogels enables the capture and concentration of target antigens present at low concentrations. Such physical entrapment provides a conceptually simple method of immobilization compared to immobilization of proteins on surfaces, and offers advantages such as resistance to chemical and thermal denaturation.Citation
Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays
Publisher Info
Methods in Molecular Biology Book Chapter, Methods in Molecular Medicine Book Series Format Guide by Humana Press,
Pub Type
Books
Keywords
antibody immobilization, hydrogel, microdevice, microfluidic immunoassays, polyacrylamide gel plug
Citation
Issues
If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.
Created October 12, 2021