Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Photopatterned Polyelectrolyte Multilayers for Cell Culture

Published

Author(s)

Darwin Reyes-Hernandez, J. Brian Hutchison, Laurie E. Locascio, Michael Gaitan

Abstract

Patterned two-dimensional cell cultures are increasingly important for constructing bioanalytical tools for cell and molecular biology to address questions about interactions, signaling, migration, and differentiation, among others. This contribution presents a unique method for patterning layer-by- layer polymer with dissimilar cell attachment characteristics. Polyelectrolyte multilayers (PEMs) were deposited and exposed with UV light through a binary photomask to form a patterned upper crosslinked stratum where cells are not viable and a lower cytophilic stratum. Specifically, bilayers of polyacrylic acid and polyacrylamide (PAA/PAAm) were patterned (i.e., crosslinked) on top of electrostatically bound layers of biocompatible poly(allylamine hydrochloride)/poly(sodium 4-styrenesulfonate (PAH/PSS) bilayers. This work demonstrates the facile patterning of PEMs for two-dimensional cell cultures that exhibit natural phenotypic expression and long-term viability, which are significant advantages over many other cell patterning strategies.
Proceedings Title
Proceedings of Micro Total Analysis Systems
Conference Dates
November 5-9, 2006
Conference Location
Tokyo, 1, JA
Conference Title
The 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (?TAS2006)

Keywords

cell culture, coating, photo polymerization, polymer, surface modification

Citation

Reyes-Hernandez, D. , Hutchison, J. , Locascio, L. and Gaitan, M. (2006), Photopatterned Polyelectrolyte Multilayers for Cell Culture, Proceedings of Micro Total Analysis Systems, Tokyo, 1, JA (Accessed February 27, 2024)
Created November 8, 2006, Updated October 12, 2021