NOTICE: Due to a lapse in annual appropriations, most of this website is not being updated. Learn more.

Form submissions will still be accepted but will not receive responses at this time. Sections of this site for programs using non-appropriated funds (such as NVLAP) or those that are excepted from the shutdown (such as CHIPS and NVD) will continue to be updated.

U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PUBLICATIONS

Microfluidic Method for Thermal Cycling by Microwave Dielectric Heating

Published

Author(s)

Pierre-Alain Auroux, Jayna J. Shah, James Booth, Mulpuri Rao, Laurie E. Locascio, Michael Gaitan

Abstract

We report on temperature time domain and spectral measurements for on-chip microwave thermocycling. In particular we observe enhanced specificity of the heating of a targeted sample compared to conventional heating block-based thermocyclers. Spatial control of the temperature in the device is implemented by delivering microwave energy to specific locations in the microfluidic chip. Our device characterization shows a linear response between the rise in temperature and the applied power as predicted by theory. On-chip thermocycling by microwave dielectric heating is characterized and compared to a commercial heating block-based thermocycler. This method is applicable to any reaction where fast and accurate heat transfer is required. We are currently developing PCR-based applications based on this technique.
Proceedings Title
The 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS)
Conference Dates
November 5-9, 2006
Conference Location
Tokyo, 1, JA
Pub Type
Conferences

Keywords

dielectric heating, DNA, microwave, PCR, thermocycling

Citation

Auroux, P. , Shah, J. , Booth, J. , Rao, M. , Locascio, L. and Gaitan, M. (2006), Microfluidic Method for Thermal Cycling by Microwave Dielectric Heating, The 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS), Tokyo, 1, JA (Accessed October 13, 2025)

Issues

If you have any questions about this publication or are having problems accessing it, please contact [email protected].

Created November 8, 2006, Updated October 12, 2021
Was this page helpful?