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Search Publications by: John P. Marino (Fed)

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Displaying 26 - 50 of 77

Neutron Scattering in the Biological Sciences: Progress and Prospects

December 1, 2018
Author(s)
Rana Ashkar, Hassina Z. Bilheux, Heliosa Bordallo, Robert Briber, David J. E. Callaway, Xiaolin Cheng, Xiang-Qiang Chu, Joseph E. Curtis, Mark Dadmun, Paul Fenimore, David Fushman, Frank Gabel, Kushol Gupta, Frederick Herberle, Frank Heinrich, Liang Hong, John Katsaras, Zvi Kelman, Eugenia Kharlampieva, Gerald R. Kneller, Andrey Kovalevsky, Susan T. Krueger, Paul Langan, Raquel Lieberman, Yun Liu, Mathias Losche, Edward Lyman, Yimin NMN Mao, John Marino, Carla Mattos, Flora Meilleur, Peter Moody, Jonathan D. Nickels, William B. O'Dell, Hugh O'Neill, Ursula Perez-Salas, Judith Peters, Loukas Petridis, Alexei P. Sokolov, Robert Sutherland, Norman Wagner, Michael NMN Weinrich, Kevin Weiss, Troy Wymore, Yang Zhang, Jeremy C. Smith
The scattering of neutrons can be used to provide information on the structure and dynamics of biological systems on multiple scales of length and time. Pursuant to an NSF-funded workshop in February 2018, we review recent developments in this field as

The role of human monoacylglycerol lipase (hMAGL) binding pocket in breakup of unsaturated phospholipid membranes

November 1, 2017
Author(s)
Ioannis L. Karageorgos, Vitalii I. Silin, nikolai zvonok, John P. Marino, David Janero, Alexandros Makriyannis
Human monoacylglycerol lipase (hMAGL) plays a key role in homeostatic tuning of the endocannabinoid signaling system and supports aggressive tumorogenesis, making this enzyme a promising therapeutic target. hMAGL features a membrane-associated lid domain

Non-Uniform Sampling for All: More Spectral Quality, Less Measurement Time

June 30, 2017
Author(s)
Frank Delaglio, Gregory S. Walker, Kathleen A. Farley, Raman Sharma, Jeffrey C. Hoch, Luke Arbogast, Robert Brinson, John Marino
Nuclear Magnetic Resonance (NMR) spectroscopy is an indispensable tool in pharmaceutical science, with uses in drug discovery, development, and manufacturing. Non-Uniform Sampling (NUS) is an acquisition method for NMR experiments with two or more

Application of 2D-NMR with Room Temperature NMR Probes for the Assessment of the Higher Order Structure of Filgrastim

January 15, 2017
Author(s)
Robert G. Brinson, Houman Ghasriani, Derek Hodgson, Adams Kristie, Ian McEwen, Kang Chen, Yves Aubin, John P. Marino
The higher order structure (HOS) of biotherapeutics is a critical quality attribute that can be evaluated by nuclear magnetic resonance (NMR) spectroscopy at atomic resolution. NMR spectral mapping of HOS can be used to establish HOS consistency of a

A New Molecular Model for Lithium's Biological Interactions

July 25, 2016
Author(s)
John Marino, Katharine T. Briggs, Gary G. Giulian, Joe Kao, Gong Li
Lithium carbonate, a drug for the treatment of Bipolar Disorder, provides mood stability to mitigate recurrent episodes of mania and/or depression. Despite its long term and widespread use, the mechanism by which lithium acts to elicit these psychological

Precision and Robustness of 2D-NMR for Structure and Assessment of Filgrastim Biosimilars

February 5, 2016
Author(s)
Houman Ghasriani, Derek Hodgson, Robert G. Brinson, Ian McEwen, Lucinda F. Buhse, Steven Kozlowski, John Marino, Yves Aubin, David Keire
The higher order structure of biotherapeutics is a critical quality attribute (CQA) that can be evaluated by nuclear magnetic resonance (NMR) spectroscopy at atomic resolution. NMR spectral "fingerprinting" can directly establish drug substance structural

Current and Emerging Technologies to Characterize the Higher-Order Structure of Monoclonal Antibodies

October 15, 2015
Author(s)
John Marino, Robert G. Brinson, Jane E. Ladner, David Travis Gallagher, Luke Arbogast, Richard Huang, Jeffrey W. Hudgens, Elyssia S. Gallagher
In contrast to small molecule therapeutics whose conformations can be absolutely defined by constitution and stereochemistry, biopharmaceuticals are distinguished by the requirement for folding into higher order structures (secondary, tertiary, and

Applying Thymine Isostere 2,4-Difluoro-5-methylbenzene as an Assignment Tool and Probe of Purine-rich DNA Dynamics Using Proton NMR Spectroscopy

June 30, 2015
Author(s)
Robert G. Brinson, Jennifer T. Miller, Kahn Jason, Stuart F. Le Grice, John P. Marino
Proton assignment of NMR spectra of oligonucleotide duplexes can become difficult with increasing helical length due to cross peak overlap. Here we describe an alternative to 15N and 13C labeling that facilitates assignment via single site substitution

2D 1HN, 15N Correlated NMR Methods at Natural Abundance for Obtaining Structural Maps and Statistical Comparability of Monoclonal Antibodies

March 1, 2015
Author(s)
Luke Arbogast, Robert G. Brinson, Catherine A. Mouchahoir, James T. Hoopes, John Marino
Purpose High-resolution nuclear magnetic resonance spectroscopy (NMR) provides a robust approach for producing unique spectral signatures of protein higher order structure at atomic resolution. Such signatures can be used as a tool to establish consistency

Mapping Monoclonal Antibody Structure by 2D 13C NMR at Natural Abundance

March 1, 2015
Author(s)
Luke Arbogast, Robert G. Brinson, John Marino
Monoclonal antibodies (mAbs) represent an important and rapidly growing class of biotherapeutics. Correct folding of a mAb is critical for drug efficacy, while misfolding can impact safety by eliciting unwanted immune or other off-target responses. Robust

Probing the intracellular glutathione redox potential using in-cell NMR spectroscopy

January 2, 2014
Author(s)
Steve Y. Rhieu, Aaron A. Urbas, Daniel W. Bearden, John P. Marino, Vytautas Reipa, Katrice A. Lippa
Non-invasive and real-time analysis of cellular redox processes has been greatly hampered by lack of suitable measurement techniques. Here we describe an in-cell nuclear magnetic resonance (NMR)-based method for measuring the intracellular glutathione

Formation of dAMP-glycerol and dAMP-Tris derivatives by the Thermococcus kodakaraensis DNA primase

May 11, 2012
Author(s)
Zvi Kelman, John P. Marino, Jerard Hurwitz, Wiebke Chemnitz Galal, Miao Pan, Gary Giulian, Wei Yuan, Shuwei Li
In the presence of dATP, glycerol and Tris buffer, the DNA primase isolated from Thermococcus kodakaraensis (Tk) catalyzed the formation of two products that were identified as dAMP-glycerol and dAMP-Tris. These products were formed by the Tk p41 catalytic

NMR Analysis of Rhodopsin-Transducin Interactions

December 1, 2006
Author(s)
Kevin Ridge, John Marino, T Ngo, Eva Ramon, D M. Brabazon, N G. Abdulaev
Heterotrimeric G-protein activation by an agonist-stimulated G-protein coupled receptor (R*) requires the propagation of structural signals from the receptor interacting surfaces to the guanine nucleotide-binding pocket. Employing high-resolution NMR