U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PUBLICATIONS

Probing the intracellular glutathione redox potential using in-cell NMR spectroscopy

Published

Author(s)

Steve Y. Rhieu, Aaron A. Urbas, Daniel W. Bearden, John P. Marino, Vytautas Reipa, Katrice A. Lippa

Abstract

Non-invasive and real-time analysis of cellular redox processes has been greatly hampered by lack of suitable measurement techniques. Here we describe an in-cell nuclear magnetic resonance (NMR)-based method for measuring the intracellular glutathione redox potential by direct and quantitative measurement of isotopically labeled glutathione introduced exogenously into living yeast. Using this approach, perturbations in the cellular glutathione redox homeostasis were also monitored as yeast cells were subjected to oxidative stress.
Citation
Nature Methods
Pub Type
Journals

Keywords

In-cell NMR, Glutathione, Non-invasive measurement, Redox potential
Bioscience, Chemistry, Analytical chemistry, Health and Spectroscopy

Citation

Rhieu, S. , Urbas, A. , Bearden, D. , Marino, J. , Reipa, V. and Lippa, K. (2014), Probing the intracellular glutathione redox potential using in-cell NMR spectroscopy, Nature Methods (Accessed December 16, 2024)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created January 2, 2014, Updated February 19, 2017