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Probing the intracellular glutathione redox potential using in-cell NMR spectroscopy

Published

Author(s)

Steve Y. Rhieu, Aaron A. Urbas, Daniel W. Bearden, John P. Marino, Vytautas Reipa, Katrice A. Lippa

Abstract

Non-invasive and real-time analysis of cellular redox processes has been greatly hampered by lack of suitable measurement techniques. Here we describe an in-cell nuclear magnetic resonance (NMR)-based method for measuring the intracellular glutathione redox potential by direct and quantitative measurement of isotopically labeled glutathione introduced exogenously into living yeast. Using this approach, perturbations in the cellular glutathione redox homeostasis were also monitored as yeast cells were subjected to oxidative stress.
Citation
Nature Methods

Keywords

In-cell NMR, Glutathione, Non-invasive measurement, Redox potential

Citation

Rhieu, S. , Urbas, A. , Bearden, D. , Marino, J. , Reipa, V. and Lippa, K. (2014), Probing the intracellular glutathione redox potential using in-cell NMR spectroscopy, Nature Methods (Accessed June 18, 2024)

Issues

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Created January 2, 2014, Updated February 19, 2017