Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

NMR Analysis of Rhodopsin-Transducin Interactions



Kevin Ridge, John Marino, T Ngo, Eva Ramon, D M. Brabazon, N G. Abdulaev


Heterotrimeric G-protein activation by an agonist-stimulated G-protein coupled receptor (R*) requires the propagation of structural signals from the receptor interacting surfaces to the guanine nucleotide-binding pocket. Employing high-resolution NMR methods, we are probing heterotrimer-associated and rhodopsin-stimulated changes in an isotope-labeled G-protein a-subunit (Ga). A key aspect of the work involves the trapping and interrogation of discrete R*-bound conformations of Ga. Our results demonstrate that functionally important changes in Ga structure and dynamics can be detected and characterized by NMR, enabling the generation of robust models for the global and local structural changes accompanying signal transfer from R* to the G-protein.
Vision Research


guanine nucleotide, NMR, Rhodopsin, signaling, transducin


Ridge, K. , Marino, J. , Ngo, T. , Ramon, E. , Brabazon, D. and Abdulaev, N. (2006), NMR Analysis of Rhodopsin-Transducin Interactions, Vision Research (Accessed June 22, 2024)


If you have any questions about this publication or are having problems accessing it, please contact

Created November 30, 2006, Updated October 12, 2021