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Formation of dAMP-glycerol and dAMP-Tris derivatives by the Thermococcus kodakaraensis DNA primase

Published

Author(s)

Zvi Kelman, John P. Marino, Jerard Hurwitz, Wiebke Chemnitz Galal, Miao Pan, Gary Giulian, Wei Yuan, Shuwei Li

Abstract

In the presence of dATP, glycerol and Tris buffer, the DNA primase isolated from Thermococcus kodakaraensis (Tk) catalyzed the formation of two products that were identified as dAMP-glycerol and dAMP-Tris. These products were formed by the Tk p41 catalytic subunit alone and the Tk p41-p46 complex in the absence of a DNA template. They were not formed with preparations containing the catalytically inactive p41 subunit. Similar glycerol and Tris derivative were also formed with dGTP, dCTP or dTTP. In addition to these derivatives, dNMPs were also formed in these reactions. The mechanism contributing to the formation of these products is discussed.
Citation
Journal of Biological Chemistry
Volume
287

Keywords

initiation reactions, nucleotide adducts, template independent

Citation

Kelman, Z. , Marino, J. , Hurwitz, J. , , W. , Pan, M. , Giulian, G. , Yuan, W. and Li, S. (2012), Formation of dAMP-glycerol and dAMP-Tris derivatives by the Thermococcus kodakaraensis DNA primase, Journal of Biological Chemistry, [online], https://doi.org/10.1074/jbc.M111.338160 (Accessed April 17, 2024)
Created May 11, 2012, Updated November 10, 2018