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Search Publications by: Lili Wang (Fed)

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Displaying 1 - 25 of 80

Standards and Metrology for Viral Vectors as Molecular Tools: Outcomes from a CCQM Workshop

May 24, 2024
Author(s)
Janathan Campbell, Neil Almond, Y Bae, Ravneet Bhuller, Andrea Briones, S-Y CHO, Megan Cleveland, Thomas Cleveland, Francis Galaway, Hua-Jun He, U Herbrand, Jim Huggett, Sarah Kempster, Ibolya Kepiro, Afifa Khan, Edward Kwee, Wilson Li, Sheng Lin-Gibson, Luise Luckau, Caterina Minelli, M Ryadnov, I Searing, Lili Wang, Alexandra Whale, Julian Braybrook
Viral vectors are agents enabling gene transfer and genome editing and have widespread utility across the healthcare and biotechnology sectors. In January 2023, the International Bureau for Weights and Measures' Consultative Committee for Amount of

Quantitative and Standardized Pseudovirus Neutralization Assay for COVID-19

April 1, 2024
Author(s)
Jerilyn Izac, Edward Kwee, Adolfas Gaigalas, Lili Wang
COVID-19 is a global pandemic caused by the highly infectious SARS-CoV-2 virus. Efforts to combat SARS-CoV-2 infection include mass vaccination and development of monoclonal and convalescent plasma therapeutics that require precise measurements of

Standardization of Flow Cytometric Detection of Antigen Expression

January 25, 2024
Author(s)
Linhua Tian, Aaron Nelson, Tyler Lowe, Linda Weaver, Constance Yuan, Paul DeRose, Maryalice Stetler-Stevenson, Lili Wang
Background: While response to antigen-based immunotherapy relies upon the level of antigen expression by tumor cells and as decreasing levels of antigen expression can be an early indicator of developing resistance to therapy, we have developed an antigen

Monoclonal Antibodies as SARS-CoV-2 Serology Standards: Experimental Validation and Broader Implications for Correlates of Protection

October 28, 2023
Author(s)
Lili Wang, Paul Patrone, Anthony Kearsley, Jerilyn Izac, Adolfas Gaigalas, John Prostko, Hang Xie, Linhua Tian, Elzafir Elsheikh, Edward Kwee, Troy Kemp, Simon Jochum, Natalie Thornburg, Clifford McDonald, Adi Gundlapalli, Sheng Lin-Gibson
COVID-19 has highlighted the need for more accurate and reproducible measurements of humoral immunity, including antibody levels and neutralization potential, which are critical for diagnostics, seroprevalence estimates, and development of vaccines and

CD19 Expression Analysis Using Two Flow cytometric Quantitative Schemes: An approach for Identifying Robust B-cell Reference Control Materials

March 19, 2021
Author(s)
Hao-Wei Wang, Paul Patrone, Anthony Kearsley, Jessica Watson, Liang Lim, Jothir Pichaandi, Olga Ornatsky, Daniel Majonis, Rukmini Bhardwaj, Howard Mostowski, Steven Bauer, Heba Degheidy, Lili Wang
Cell-based therapies have emerged as a novel approach to treat cancer and other conditions. Quantitative flow cytometry plays an increasingly important role in predicting therapy outcome in a wide range of hematologic malignancies. Several studies

Towards Quantitative and Standardized Serological and Neutralization Assays for COVID-19

March 8, 2021
Author(s)
Linhua Tian, Elzafir B. Elsheikh, Paul Patrone, Anthony Kearsley, adolfas Gaigalas, Sarah L. Inwood, Sheng Lin-Gibson, Dominic Esposito, Lili Wang
Quantitative and robust serology assays are critical measurements underpinning global COVID-19 response to diagnostic, surveillance, and vaccine development. Here, we report a proof-of-concept approach for the development of quantitative, multiplexed flow

Introduction and Clinical Validation of Metrology Standards for Immunohistochemistry

December 21, 2020
Author(s)
Emina E. Torlakovic, Seshi Sompuram, Vani Kodela, Anika Schaedle, Paul DeRose, Steve Bogen, Lili Wang
Despite the widespread integration of immunohistochemistry (IHC) into routine surgical pathology, its use suffers from significant inter-laboratory disparities. The root cause and an unsolved technical challenge is the lack of metrology standards, the

Towards defining reference materials for extracellular vesicle size, concentration, refractive index and epitope abundance

September 24, 2020
Author(s)
Joshua Welsh, Edwin van der Pol, Britta Bettin, David Carter, An Hendrix, Metka Lenassi, Marc-Andre Langlois, Alicia Liorente, Arthur van de Nes, Rienk Nieuwland, Vera Tang, Kenneth Witwer, Jennifer Jones, Lili Wang
Accurate characterization of extracellular vesicles (EVs) is critical to explore their diagnostic and therapeutic applications. As the EV research field has developed, so too have the techniques used to characterize them. The development of reference

Workshop 13: Building Measurement Assurance in Flow Cytometry

June 17, 2019
Author(s)
Lili Wang, Stephen Perfetto, Robert Hoffman, John T. Elliott, Sheng Lin-Gibson, Steven Bauer, Heba Degheidy, Judith Arcidiacono, Litwin Virginia
Two workshops were held to identify measurement challenges and potential solutions for building measurement assurance for flow cytometry. This report summarizes key findings, including the need for high quality reagents, reference standards or materials

Workshop 9: Control Cells or Not

June 17, 2019
Author(s)
Paul Wallace, Jonni S. Moore, Derek Jones, Litwin Virginia, Lili Wang, Yanli Liu
"Control Cells or Not" was an educational workshop that used surveys, lectures, and discussions to identify problems and solutions related to using commercially available control cell products and lab-developed approaches. Collective efforts are proposed

Comparison of volumetric and bead-based counting of CD34 cells by single-platform flow cytometry

February 20, 2019
Author(s)
Luisa Saraiva, Lili Wang, Martin Kammel, A. Kummrow, E Atkinson, J Y. Lee, B Yalcinkaya, M Akgoz, A Ruf, A Engel, Yu-Zhong Zhang, O O''Shea, M P. Sassi, C Divieto, T Lekishvili, J J. Campbell, Y Liu, J Wang, R Stebbings, Adolfas Gaigalas, P Rigsby, J Neukammer, S Vessillier
Background: Over 2000 people a year in the UK need a bone marrow or blood stem cell transplant. It is important to accurately quantify the haematopoietic stem cells to predict whether the transplant will be successful in replenishing the immune system

Methodology for evaluating and comparing fluorescence measurement capabilities: Multi-site study of 23 flow cytometers

September 23, 2018
Author(s)
David R. Parks, Wayne A. Moore, Ryan Brinkman, Yong Chen, Danilo Condello, Faysal E. Khettabi, John P. Nolan, Stephen P. Perfetto, Doug Redelman, Josef Spidlen, Jonathan V. Dyke, Lili Wang, James C. Wood
We developed measurement methods and analysis procedures to perform inter-instrument comparisons and used them to evaluate and compare the fluorescence measurement capabilities among 23 cytometers in 9 laboratories selected to provide a range of

Quantitative Fluorescence Measurements with Multicolor Flow Cytometry

November 20, 2017
Author(s)
Lili Wang, Adolfas K. Gaigalas, James Wood
Multicolor flow cytometer assays are routinely used in clinical laboratories for immunophenotyping, monitoring disease and treatment, and determining prognostic factors. However, existing methods for quantitative measurements have not yet produced

Quantitative Flow Cytometry Measurements in Antibody Bound per Cell Based on CD4 Reference

February 12, 2016
Author(s)
Lili Wang, Heba Degheidy, Fatima Abbasi, Howard Mostowski, Gerald Marti, Steven R. Bauer, Robert Hoffman, Adolfas K. Gaigalas
Multicolor flow cytometer assays are with fluorescently labeled antibodies routinely used in clinical laboratories to measure the cell number of specific immunophenotypes and to estimate expression levels of specific receptors/antigens either on the cell
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