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Search Publications by: Lili Wang (Fed)

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Displaying 26 - 50 of 80

Quantification of Cells with Specific Phenotypes I: Determination of CD4+ Cell Count per mL in Reconstituted Lyophilized Human PBMC Pre-labelled with Anti-CD4 FITC Antibody

March 10, 2015
Author(s)
Richard Stebbings, Lili Wang, Janet Sutherland, Martin Kammel, Adolfas Gaigalas, M. John, B. Roemer, M. Kuhne, R. J. Schneider, M. Braun, N. Leclere, D. Dikshit, Fatima Abbasi, Gerald Marti, P. Porcedda, M. Sassi, L. Revel, S. K. Kim, D. Marshall, L. Whitby, Jing Wang, V. Ost, M. Vonski, Joerg Neukammer
A surface-labelled lyophilised lymphocyte (sLL) preparation has been developed using human peripheral blood mononuclear cells (PBMC) pre-labelled with a fluorescein isothiocyanate (FITC) conjugated anti-CD4 monoclonal antibody. The sLL preparation is

Quantifying CD4 receptor protein in two human CD4+ lymphocyte preparations for quantitative flow cytometry

December 11, 2014
Author(s)
Meiyao M. Wang, Martin Misakian, Hua-Jun He, Peter Bajcsy, Jeffrey M. Davis, Kenneth D. Cole, Illarion Turko, Lili Wang, Fatima Abbasi
For quantitative flow cytometry, a biological cell reference material with a known biomarker expression level is needed to transform a linear arbitrary fluorescence intensity scale obtained with fluorescent microspheres to an antibody bound per cell (ABC)

Flow Cytometer Performance Characterization, Standardization and Calibration against CD4 on T Lymphocytes Enables Quantification of Biomarker Expressions for Immunological Applications

August 7, 2014
Author(s)
Heba Degheidy, Steven R. Bauer, Gerald Marti, Lili Wang
There is an urgent need for developing a procedure for biomarker standardization and quantification in clinical laboratories. Measuring the expression levels of cell antigens is critical for the diagnosis of many diseases, e.g. leukemia, lymphoma and

Breast Cancer Biomarker Measurements and Standards

January 24, 2013
Author(s)
Kenneth D. Cole, Lili Wang, Hua-Jun He
Cancer is a heterogeneous disease characterized by changes in the levels and activities of important cellular proteins, including oncogenes and tumor suppressors. Genetic mutations cause changes in protein activity and protein expression levels that result

Negative Regulation of STAT3-mediated Cellular Respiration by SirT1

June 7, 2011
Author(s)
Lili Wang, Hua-Jun He, Michel Bernier, Rajib K. Paul, Alejandro Martin-Montalvo, Morten Scheibye-Knudsen, Shaoming Song, Sean M. Armour, Vilhelm A. Bohr, Yaping Zong, David A. Sinclair, Rafael de Cabo
In mammals, the transcriptional activity of signal transducer and activator of transcription 3 (STAT3) is regulated by the deacetylase SirT1. However, whether the newly described non-genomic actions of STAT3 toward mitochondrial oxidative phosphorylation

Variables in the Quantification of CD4 in Normals and Hairy Cell Leukemia Patients

January 3, 2011
Author(s)
Gerald E. Marti, William G. Stetler-Stevenson, Fatima Abbasi, Gregory A. Jasper, Robert J. Kreitman, Lili Wang
Background: Quantitative flow cytometry (QFCM) is being applied in the clinical flow cytometry laboratory. Quantitative normal T-cell CD4 expression represents a biologic standard and quality control agent. However, Low levels of CD4 expression were

Development of a Candidate Secondary Reference Procedure (immunoassay based measurement procedure of higher metrological order) for Cardiac Troponin I: I. Antibody Characterization and Preliminary Validation

November 1, 2010
Author(s)
James E. Noble, David M. Bunk, R. H. Christenson, Kenneth D. Cole, Hua-Jun He, Alexei Katrukha, Mauro Panteghini, Robert Porter, Heinz Schimmel, Jillian Tate, Lili Wang
The development of a reference measurement procedure to support the cardiac troponin I (cTnI) standardization initiative is described. The reference measurement procedure will be used to assign reference values to serum-based secondary standards. A

Sensing the Insulin Signaling Pathway with an Antibody Array

December 1, 2009
Author(s)
Hua-Jun He, Y Zong, Michel Bernier, Lili Wang
Defects within the insulin signaling pathways are a major locus for the development of insulin resistance and type 2 diabetes. The phorbol 12-myristate 13-acetate (PMA) is commonly described as a mediator of insulin resistance through the activation of the

Modeling of Photochemical Reactions in a Focused Laser Beam, II

September 1, 2008
Author(s)
Adolfas K. Gaigalas, Fern Y. Hunt, Lili Wang
A method is described for obtaining rate constants of photodegradation process of fluorophores illuminated by a focused laser beam. The explicit kinetic equations, describing the population dynamics of excited singlet and triplet states, are averaged over

An international comparability study to determine the sources of uncertainty associated with a non-competitive sandwich fluorescent ELISA

July 1, 2008
Author(s)
James E. Noble, Lili Wang, Eleonora Cerasoli, Alex E. Knight, Robert A. Porter, Elaine Gray, Chris Howe, Elisabeth Hannes, Philippe Corbisier, Jing Wang, Liqing Wu, Ilaria Altieri, Marina Patriarca, Angelika Hoffman, Ute Resch-Genger, Bernd Ebert, Jan Voigt, Yasushi Shigeri, Maxim S. Vonsky, L A. Konopelko, Adolfas K. Gaigalas, Bailey J. Marc

Need for and Metrological Approaches Towards Standardization of Fluorescence Measurements From the View of National Metrology Institutes

April 9, 2008
Author(s)
Paul C. DeRose, Lili Wang, Adolfas K. Gaigalas, Gary W. Kramer, Ute Resch-Genger, Ulrich Panne
The need for standardization in fluorescence measurements to improve quality assurance and to meet regulatory demands is addressed from the viewpoint of national metrological institutes (NMIs). Classes of fluorescence standards are defined, including
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