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Search Publications by: Lili Wang (Fed)

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Displaying 26 - 50 of 72

Negative Regulation of STAT3-mediated Cellular Respiration by SirT1

June 7, 2011
Lili Wang, Hua-Jun He, Michel Bernier, Rajib K. Paul, Alejandro Martin-Montalvo, Morten Scheibye-Knudsen, Shaoming Song, Sean M. Armour, Vilhelm A. Bohr, Yaping Zong, David A. Sinclair, Rafael de Cabo
In mammals, the transcriptional activity of signal transducer and activator of transcription 3 (STAT3) is regulated by the deacetylase SirT1. However, whether the newly described non-genomic actions of STAT3 toward mitochondrial oxidative phosphorylation

Variables in the Quantification of CD4 in Normals and Hairy Cell Leukemia Patients

January 3, 2011
Gerald E. Marti, William G. Stetler-Stevenson, Fatima Abbasi, Gregory A. Jasper, Robert J. Kreitman, Lili Wang
Background: Quantitative flow cytometry (QFCM) is being applied in the clinical flow cytometry laboratory. Quantitative normal T-cell CD4 expression represents a biologic standard and quality control agent. However, Low levels of CD4 expression were

Development of a Candidate Secondary Reference Procedure (immunoassay based measurement procedure of higher metrological order) for Cardiac Troponin I: I. Antibody Characterization and Preliminary Validation

November 1, 2010
James E. Noble, David M. Bunk, R. H. Christenson, Kenneth D. Cole, Hua-Jun He, Alexei Katrukha, Mauro Panteghini, Robert Porter, Heinz Schimmel, Jillian Tate, Lili Wang
The development of a reference measurement procedure to support the cardiac troponin I (cTnI) standardization initiative is described. The reference measurement procedure will be used to assign reference values to serum-based secondary standards. A

Sensing the Insulin Signaling Pathway with an Antibody Array

December 1, 2009
Hua-Jun He, Y Zong, Michel Bernier, Lili Wang
Defects within the insulin signaling pathways are a major locus for the development of insulin resistance and type 2 diabetes. The phorbol 12-myristate 13-acetate (PMA) is commonly described as a mediator of insulin resistance through the activation of the

Modeling of Photochemical Reactions in a Focused Laser Beam, II

September 1, 2008
Adolfas K. Gaigalas, Fern Y. Hunt, Lili Wang
A method is described for obtaining rate constants of photodegradation process of fluorophores illuminated by a focused laser beam. The explicit kinetic equations, describing the population dynamics of excited singlet and triplet states, are averaged over

An international comparability study to determine the sources of uncertainty associated with a non-competitive sandwich fluorescent ELISA

July 1, 2008
James E. Noble, Lili Wang, Eleonora Cerasoli, Alex E. Knight, Robert A. Porter, Elaine Gray, Chris Howe, Elisabeth Hannes, Philippe Corbisier, Jing Wang, Liqing Wu, Ilaria Altieri, Marina Patriarca, Angelika Hoffman, Ute Resch-Genger, Bernd Ebert, Jan Voigt, Yasushi Shigeri, Maxim S. Vonsky, L A. Konopelko, Adolfas K. Gaigalas, Bailey J. Marc

Need for and Metrological Approaches Towards Standardization of Fluorescence Measurements From the View of National Metrology Institutes

April 9, 2008
Paul C. DeRose, Lili Wang, Adolfas K. Gaigalas, Gary W. Kramer, Ute Resch-Genger, Ulrich Panne
The need for standardization in fluorescence measurements to improve quality assurance and to meet regulatory demands is addressed from the viewpoint of national metrological institutes (NMIs). Classes of fluorescence standards are defined, including

Toward Quantitative Fluorescence Measurements with Multicolor Flow Cytometry

December 28, 2007
Lili Wang, Adolfas Gaigalas, Gerald Marti, Fatima Abbasi, Robert A. Hoffman
A procedure is presented for calibrating the output of a multicolor flow cytometer in units of antibodies bound per cell (ABC). The procedure involves two steps. First, each of the fluorescence channels of the flow cytometer is calibrated using Ultra

Epitope Selection of Monoclonal Antibodies for Interleukin-4 Quantification Using Suspension Arrays and Forward-Phase Protein Microarrays

December 6, 2007
Lili Wang, Kenneth D. Cole, Hua-Jun He, Alexander W. Peterson, Adolfas K. Gaigalas, Y Zong
A recombinant mouse interleukin-4 (IL-4) and three different purified rat anti-mouse IL-4 monoclonal antibodies (Mab) with different epitopes were employed as a model system. This system was used to examine monoclonal antibody effectiveness using both

Porous Silicon Biosensor for MS2 Virus

November 19, 2007
Andrea Rossi, Lili Wang, Vytas Reipa, Thomas E. Murphy
Nanoporous silicon was discovered in the 1950 s and has unique properties due to quantum confinement effects. It is produced by electrochemical etching in HF containing electrolytes that allows to tune the pore diameter from a few nanometers up to several

Modeling of Photochemical Reactions in a Focused Laser Beam

July 1, 2007
Fern Y. Hunt, Adolfas Gaigalas, Lili Wang
This paper presents a mathematical model of photodegradation of fluorophores passing through a laser beam. The beam is focused on the sample and thus the power distribution of incident light is strongly dependent on spatial location while the fluorophores