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Search Publications by: Lili Wang (Fed)

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Displaying 51 - 75 of 85

Modeling of Photochemical Reactions in a Focused Laser Beam, II

September 1, 2008
Author(s)
Adolfas K. Gaigalas, Fern Y. Hunt, Lili Wang
A method is described for obtaining rate constants of photodegradation process of fluorophores illuminated by a focused laser beam. The explicit kinetic equations, describing the population dynamics of excited singlet and triplet states, are averaged over

An international comparability study to determine the sources of uncertainty associated with a non-competitive sandwich fluorescent ELISA

July 1, 2008
Author(s)
James E. Noble, Lili Wang, Eleonora Cerasoli, Alex E. Knight, Robert A. Porter, Elaine Gray, Chris Howe, Elisabeth Hannes, Philippe Corbisier, Jing Wang, Liqing Wu, Ilaria Altieri, Marina Patriarca, Angelika Hoffman, Ute Resch-Genger, Bernd Ebert, Jan Voigt, Yasushi Shigeri, Maxim S. Vonsky, L A. Konopelko, Adolfas K. Gaigalas, Bailey J. Marc

Need for and Metrological Approaches Towards Standardization of Fluorescence Measurements From the View of National Metrology Institutes

April 9, 2008
Author(s)
Paul C. DeRose, Lili Wang, Adolfas K. Gaigalas, Gary W. Kramer, Ute Resch-Genger, Ulrich Panne
The need for standardization in fluorescence measurements to improve quality assurance and to meet regulatory demands is addressed from the viewpoint of national metrological institutes (NMIs). Classes of fluorescence standards are defined, including

Toward Quantitative Fluorescence Measurements with Multicolor Flow Cytometry

December 28, 2007
Author(s)
Lili Wang, Adolfas Gaigalas, Gerald Marti, Fatima Abbasi, Robert A. Hoffman
A procedure is presented for calibrating the output of a multicolor flow cytometer in units of antibodies bound per cell (ABC). The procedure involves two steps. First, each of the fluorescence channels of the flow cytometer is calibrated using Ultra

Epitope Selection of Monoclonal Antibodies for Interleukin-4 Quantification Using Suspension Arrays and Forward-Phase Protein Microarrays

December 6, 2007
Author(s)
Lili Wang, Kenneth D. Cole, Hua-Jun He, Alexander W. Peterson, Adolfas K. Gaigalas, Y Zong
A recombinant mouse interleukin-4 (IL-4) and three different purified rat anti-mouse IL-4 monoclonal antibodies (Mab) with different epitopes were employed as a model system. This system was used to examine monoclonal antibody effectiveness using both

Porous Silicon Biosensor for MS2 Virus

November 19, 2007
Author(s)
Andrea Rossi, Lili Wang, Vytas Reipa, Thomas E. Murphy
Nanoporous silicon was discovered in the 1950 s and has unique properties due to quantum confinement effects. It is produced by electrochemical etching in HF containing electrolytes that allows to tune the pore diameter from a few nanometers up to several

Modeling of Photochemical Reactions in a Focused Laser Beam

July 1, 2007
Author(s)
Fern Y. Hunt, Adolfas Gaigalas, Lili Wang
This paper presents a mathematical model of photodegradation of fluorophores passing through a laser beam. The beam is focused on the sample and thus the power distribution of incident light is strongly dependent on spatial location while the fluorophores

Methods to Characterize Ricin for the Development of Reference Materials

July 1, 2006
Author(s)
Sung Kim, Diane K. Hancock, Lili Wang, Kenneth D. Cole, Prasad T. Reddy
Ricin is an abundant protein from the castor bean plant Ricinus communis. Because of its high toxicity and the simplicity of producing mass quantities, ricin is considered a biological terrorism agent. We have characterized ricin extensively with a view to

Effect of Target and Probe Concentrations on Hybridization in DNA Microarrays

June 1, 2004
Author(s)
Lili Wang, Adolfas K. Gaigalas, Y Zong, S Zhang, J Shi, Yunda Wang
Efficient hybridization of complementary strands of DNA is the underlying principle of all microarray-based techniques for gene expression analysis. Recently studies have been published to assess oligonucleotide (55-70 bases) performance on glass-slide
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