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Search Publications by: Lili Wang (Fed)

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Displaying 51 - 75 of 80

Toward Quantitative Fluorescence Measurements with Multicolor Flow Cytometry

December 28, 2007
Author(s)
Lili Wang, Adolfas Gaigalas, Gerald Marti, Fatima Abbasi, Robert A. Hoffman
A procedure is presented for calibrating the output of a multicolor flow cytometer in units of antibodies bound per cell (ABC). The procedure involves two steps. First, each of the fluorescence channels of the flow cytometer is calibrated using Ultra

Epitope Selection of Monoclonal Antibodies for Interleukin-4 Quantification Using Suspension Arrays and Forward-Phase Protein Microarrays

December 6, 2007
Author(s)
Lili Wang, Kenneth D. Cole, Hua-Jun He, Alexander W. Peterson, Adolfas K. Gaigalas, Y Zong
A recombinant mouse interleukin-4 (IL-4) and three different purified rat anti-mouse IL-4 monoclonal antibodies (Mab) with different epitopes were employed as a model system. This system was used to examine monoclonal antibody effectiveness using both

Porous Silicon Biosensor for MS2 Virus

November 19, 2007
Author(s)
Andrea Rossi, Lili Wang, Vytas Reipa, Thomas E. Murphy
Nanoporous silicon was discovered in the 1950 s and has unique properties due to quantum confinement effects. It is produced by electrochemical etching in HF containing electrolytes that allows to tune the pore diameter from a few nanometers up to several

Modeling of Photochemical Reactions in a Focused Laser Beam

July 1, 2007
Author(s)
Fern Y. Hunt, Adolfas Gaigalas, Lili Wang
This paper presents a mathematical model of photodegradation of fluorophores passing through a laser beam. The beam is focused on the sample and thus the power distribution of incident light is strongly dependent on spatial location while the fluorophores

Methods to Characterize Ricin for the Development of Reference Materials

July 1, 2006
Author(s)
Sung Kim, Diane K. Hancock, Lili Wang, Kenneth D. Cole, Prasad T. Reddy
Ricin is an abundant protein from the castor bean plant Ricinus communis. Because of its high toxicity and the simplicity of producing mass quantities, ricin is considered a biological terrorism agent. We have characterized ricin extensively with a view to

Effect of Target and Probe Concentrations on Hybridization in DNA Microarrays

June 1, 2004
Author(s)
Lili Wang, Adolfas K. Gaigalas, Y Zong, S Zhang, J Shi, Yunda Wang
Efficient hybridization of complementary strands of DNA is the underlying principle of all microarray-based techniques for gene expression analysis. Recently studies have been published to assess oligonucleotide (55-70 bases) performance on glass-slide

Formalization of the MESF Unit of Fluorescence Intensity

January 1, 2004
Author(s)
A. Schwartz, Adolfas Gaigalas, Lili Wang, G E. Marti, R F. Vogt, R Fernandez-Prini
This report summarizes the work performed during the past two years at the National Institute of Standards and Technology (NIST) in the refinement and formal definition of the MESF unit of fluorescence intensity. In addition to the theory underlying the

Fluorescence Resonance Energy Transfer Between Donor-Acceptor Pair on Two Oligonucleotides Hybridized Adjacently to a DNA Template

January 1, 2003
Author(s)
Lili Wang, Adolfas K. Gaigalas, J L. Blasic, Marcia J. Holden, David T. Gallagher, R Pires
We have used fluorescein as the energy donor and rhodamine as the acceptor to measure the efficiency of fluorescence resonance energy transfer (FRET) in a set of hybridized DNA constructs. The two fluorophores are covalently attached via linkers to two

Frequency-Domain Measurement of the Photodegradation Process of Fluorescein

July 1, 2002
Author(s)
Adolfas K. Gaigalas, Lili Wang, R F. Vogt
The frequency domain technique is applied to measure the photodegrdation rate offluorescein in aqueous solutions. The illuminating light is modulated and the changes in fluorescence from the illuminated region are detected synchronously. A constant flow

Quantitating Fluorescence Intensity From Fluorophores: Practical Use of MESF Values

July 1, 2002
Author(s)
Lili Wang, Adolfas K. Gaigalas, F Abassi, G E. Marti, R F. Vogt, A Schwarz
The present work uses fluorescein as the model fluorophore and points out critical steps in the use of MESF (Molecules of Equivalent Soluble Fluorophores) values for quantitative flow cytomeric measurements. It has been found that the emission spectrum
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