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PUBLICATIONS

NIST SP260 - Assignment of Fluorescence Intensity to Calibration Microspheres and Nanospheres for Flow Cytometry

Published

Author(s)

Paul DeRose, Linhua Tian, Ningchun Xu, Lili Wang

Abstract

The latest protocols are described for the NIST assignment of fluorescence intensity to calibration spheres or beads, which are used primarily for the accurate measurement of particle intensity in quantitative flow cytometry. Procedures for measuring fluorescence intensity and bead concentration are described and the history of these assignments for the NIST Flow Cytometry Standards Consortium and its members are reviewed.
Citation
Special Publication (NIST SP) - 260-264
Report Number
260-264
NIST Pub Series
Special Publication (NIST SP)
Pub Type
NIST Pubs

Download Paper

https://doi.org/10.6028/NIST.SP.260-264
Local Download

Keywords

beads, calibration, cytometer, flow cytometry, fluorescence channels, intensity, microspheres, nanospheres, standards
Standards, Metrology, Health and Bioscience

Citation

DeRose, P. , Tian, L. , Xu, N. and Wang, L. (2026), NIST SP260 - Assignment of Fluorescence Intensity to Calibration Microspheres and Nanospheres for Flow Cytometry, Special Publication (NIST SP), National Institute of Standards and Technology, Gaithersburg, MD, [online], https://doi.org/10.6028/NIST.SP.260-264, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=960774 (Accessed January 8, 2026)

Issues

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Created January 7, 2026
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