Izac, J.
, Kwee, E.
, Tian, L.
, Elliott, J.
and Wang, L.
(2023),
Development of a Cell-Based SARS-CoV-2 Pseudovirus Neutralization Assay Using Imaging and Flow Cytometry Analysis, International Journal of Molecular Sciences, [online], https://doi.org/10.3390/ijms241512332, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=935866
(Accessed September 18, 2025)
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Development of a Cell-Based SARS-CoV-2 Pseudovirus Neutralization Assay Using Imaging and Flow Cytometry Analysis
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Abstract
COVID-19 is an ongoing, global pandemic caused by the novel, highly infectious SARS-CoV-2 virus. Efforts to dampen the effects of SARS-CoV-2, such as mass vaccination and development of monoclonal therapeutics, require precise measurements of correlative, functional neutralizing antibodies that block virus infection. Developing rapid, safe, and easy-to-use neutralization assays are important. Here, we developed a vesicular stomatitis virus-based neutralization assay with two readout methods, imaging and flow cytometry based, that were capable of quantifying varying degrees of neutralization in patient serum samples. The two methods produced similar 50% neutralization titer values, demonstrating the utility of both readout methods.Citation
International Journal of Molecular Sciences
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Journals
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Keywords
SARS-CoV-2, COVID-19, flow cytometry, pseudovirus neutralization assay, imaging
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Created August 2, 2023, Updated August 28, 2025