NOTICE: Due to a lapse in annual appropriations, most of this website is not being updated. Learn more.

Form submissions will still be accepted but will not receive responses at this time. Sections of this site for programs using non-appropriated funds (such as NVLAP) or those that are excepted from the shutdown (such as CHIPS and NVD) will continue to be updated.

U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PUBLICATIONS

Development of a Cell-Based SARS-CoV-2 Pseudovirus Neutralization Assay Using Imaging and Flow Cytometry Analysis

Published

Author(s)

Jerilyn Izac, Edward Kwee, Linhua Tian, John Elliott, Lili Wang

Abstract

COVID-19 is an ongoing, global pandemic caused by the novel, highly infectious SARS-CoV-2 virus. Efforts to dampen the effects of SARS-CoV-2, such as mass vaccination and development of monoclonal therapeutics, require precise measurements of correlative, functional neutralizing antibodies that block virus infection. Developing rapid, safe, and easy-to-use neutralization assays are important. Here, we developed a vesicular stomatitis virus-based neutralization assay with two readout methods, imaging and flow cytometry based, that were capable of quantifying varying degrees of neutralization in patient serum samples. The two methods produced similar 50% neutralization titer values, demonstrating the utility of both readout methods.
Citation
International Journal of Molecular Sciences
Pub Type
Journals

Download Paper

https://doi.org/10.3390/ijms241512332
Local Download

Keywords

SARS-CoV-2, COVID-19, flow cytometry, pseudovirus neutralization assay, imaging
Standards, Precision medicine, Health, Flow cytometry, Cell measurements, Bioscience, Biometrology and Bioimaging

Citation

Izac, J. , Kwee, E. , Tian, L. , Elliott, J. and Wang, L. (2023), Development of a Cell-Based SARS-CoV-2 Pseudovirus Neutralization Assay Using Imaging and Flow Cytometry Analysis, International Journal of Molecular Sciences, [online], https://doi.org/10.3390/ijms241512332, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=935866 (Accessed October 7, 2025)

Issues

If you have any questions about this publication or are having problems accessing it, please contact [email protected].

Created August 2, 2023, Updated August 28, 2025
Was this page helpful?