Towards defining reference materials for extracellular vesicle size, concentration, refractive index and epitope abundance

Published

September 24, 2020

Author(s)

Joshua Welsh, Edwin van der Pol, Britta Bettin, David Carter, An Hendrix, Metka Lenassi, Marc-Andre Langlois, Alicia Liorente, Arthur van de Nes, Rienk Nieuwland, Vera Tang, Kenneth Witwer, Jennifer Jones, Lili Wang

Abstract

Accurate characterization of extracellular vesicles (EVs) is critical to explore their diagnostic and therapeutic applications. As the EV research field has developed, so too have the techniques used to characterize them. The development of reference materials is required for the standardization of these techniques. This work, initiated from the ISEV 2017 Biomarker Workshop in Birmingham, UK, and with further discussion during the ISEV 2019 Standardization Workshop in Ghent, Belgium, sets out to elucidate which reference materials are required and which are currently available to standardize commonly used analysis platforms for characterizing EV size, concentration, refractive index, and epitope expression. Due to their predominant use, a particular focus is placed on the optical methods nanoparticle tracking analysis and flow cytometry.

Citation

Journal of Extracellular Vesicles

Pub Type

Journals

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Keywords

calibration, exosomes, extracellular vesicles, microvesicles, optical analysis, reference materials, standardization, quality control, validation

Citation

Welsh, J. , van der Pol, E. , Bettin, B. , Carter, D. , Hendrix, A. , Lenassi, M. , Langlois, M. , Liorente, A. , van de Nes, A. , Nieuwland, R. , Tang, V. , Witwer, K. , Jones, J. and Wang, L. (2020), Towards defining reference materials for extracellular vesicle size, concentration, refractive index and epitope abundance, Journal of Extracellular Vesicles, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=929882 (Accessed October 2, 2025)