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Health

Pharmaceuticals

Search Publications

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  • Published Date
Displaying 76 - 100 of 122

Determining carbapenemase activity with 18O labeling and targeted mass spectrometry

October 16, 2013
Author(s)
Meiyao Wang, Yang Shen, Illarion Turko, Daniel Nelson, Shuwei Li
Carbapenems are broad spectrum antibiotics considered as last resort drugs to treat bacterial infections. Carbapenem-hydrolyzing β-lactamases (also called carbapenemases), however, can confer bacterial resistance and represent a serious health threat. Here

Effects of Desialylation on Human alpha1-Acid Glycoprotein-Ligand Interactions

September 16, 2013
Author(s)
Richard Y. Huang, Jeffrey W. Hudgens
Human α1-Acid Glycoprotein (AGP), an acute phase glycoprotein, exists predominantly in blood. With its ability to bind basic, lipophilic, and acidic drugs, AGP has served as a drug carrier. It has been shown that the carbohydrate composition of AGP changes

Multicomponent Chemical Imaging of Pharmaceutical Solid Dosage Forms with Broadband CARS Microscopy

July 15, 2013
Author(s)
Christopher M. Hartshorn, Marcus T. Cicerone, Young J. Lee, Charles H. Camp, John M. Heddleston, Patrick Marsac, Tim Rhodes, Zhen Liu, Nicole Canfield
We compare broadband coherent anti-Stokes Raman scattering (BCARS) microscopy with spontaneous Raman microscopy for quantitative and qualitative assessment of multicomponent pharmaceuticals. Indomethacin was used as a model active pharmaceutical ingredient

The Metrology of Counting Protein Particles

July 13, 2013
Author(s)
Dean C. Ripple, Michael J. Carrier, Richard E. Cavicchi, Christopher B. Montgomery, Zhishang Hu
A common degradation pathway for protein-based drugs is the growth of protein aggregates or particles. Counting and characterization of these particles is needed to assure the quality, efficacy, and safety of this type of drug. The unusual physical

Microfluidic Synthesis of Nano-Liposomal Anesthetics

May 12, 2013
Author(s)
Wyatt N. Vreeland, Renee R. Hood, Eric Kendall, Mariana Junqueira, Julia Finkel, Don L. DeVoe
Topical anesthesia has the ability to provide painless application of pain management medications for minor procedures such as venipuncture, particularly in the pediatric population. Liposomes have been proposed as a highly advantageous nanocarrier for

The Metrology of Counting Protein Particles

March 18, 2013
Author(s)
Dean C. Ripple, Michael J. Carrier, Richard E. Cavicchi, Christopher B. Montgomery, Zhishang Hu
A common degradation pathway for protein-based drugs is the growth of protein aggregates or particles. Counting and characterization of these particles is needed to assure the quality, efficacy, and safety of this type of drug. The unusual physical

Modification of tethered bilayers by phospholipid exchange with vesicles

February 27, 2013
Author(s)
David J. Vanderah, Rima Budvytyte, Mindaugas Mickevicius, Gintaras Valincius, Frank Heinrich
Phosphatidylcholine and cholesterol exchange between vesicles and planar tethered bilayer lipid membranes (tBLMs) was demonstrated from electrochemical impedance spectroscopy (EIS), fluorescence microscopy (FM), and neutron reflectometry (NR) data

Breast Cancer Biomarker Measurements and Standards

January 24, 2013
Author(s)
Kenneth D. Cole, Lili Wang, Hua-Jun He
Cancer is a heterogeneous disease characterized by changes in the levels and activities of important cellular proteins, including oncogenes and tumor suppressors. Genetic mutations cause changes in protein activity and protein expression levels that result

Quantifying Dithiothreitol Displacement of Functional Ligands from Gold Nanoparticles

October 27, 2012
Author(s)
De-Hao D. Tsai, Frank W. DelRio, Sherrie R. Elzey, Suvajyoti S. Guha, Michael R. Zachariah, Vincent A. Hackley, Melanie P. Shelton
Dithiothreitol (DTT)-based displacement is widely utilized for separating ligands from their gold nanoparticle (AuNP) conjugates, a critical step for differentiating and quantifying surface-bound functional ligands and therefore the effective surface

Evaluation of viability and proliferation profiles on macrophages treated with silica nanoparticles in vitro via plate-based, flow cytometry, and Coulter counter assays

September 3, 2012
Author(s)
Simona Bancos, De-Hao D. Tsai, Vincent A. Hackley, J L. Weaver, Katherine M. Tyner
Nanoparticles (NPs) are known to interfere with many high throughput cell viability and cell proliferation assays, which complicates the assessment of their potential toxic effects. The aim of this study was to compare viability and proliferation results

Thermal Analysis of Refrigeration Systems Used for Vaccine Storage

August 2, 2012
Author(s)
Michal J. Chojnacky, Gregory F. Strouse
The CDC administers over $3 billion worth of vaccines per year through the Vaccines for Children program. Vaccine storage temperature is critical to maintaining drug potency, and as much as one-third of delivered vaccines are wasted due to thermal

Protein Particles: What We Know and What We Dont Know

June 26, 2012
Author(s)
Dean C. Ripple, Mariana Dimitrova
All therapeutic protein products contain particles formed by the aggregation of protein monomers. There is a growing interest in the understanding of particles in biopharmaceutical products, which has been fostered on the one hand by significant

A Lectin-Based Gold Nanoparticle Assay for Probing Glycosylation of Glycoproteins

April 17, 2012
Author(s)
Germarie Sanchez-Pomales, Todd A. Morris, James Falabella, Michael J. Tarlov, Rebecca A. Zangmeister
We report a glycoanalysis method in which lectins are used to probe the glycans of glycoproteins that are adsorbed on gold nanoparticles. A model mannose-presenting glycoprotein, ribonuclease B (RNase B), and the therapeutic monoclonal antibody (mAb)

Tumor Necrosis Factor Interaction with Gold Nanoparticles

March 14, 2012
Author(s)
De-Hao D. Tsai, Sherrie R. Elzey, Frank W. DelRio, Robert I. MacCuspie, Suvajyoti S. Guha, Michael R. Zachariah, Athena M. Keene, Jeffrey D. Clogston, Vincent A. Hackley
We report on a systematic investigation of molecular conjugation of tumor necrosis factor protein-α (TNF) onto gold nanoparticles (AuNPs) and the subsequent binding behavior to its antibody (anti-TNF). We employ a combination of physical and spectroscopic

Standards for the Optical Detection of Protein Particulates

January 23, 2012
Author(s)
Dean C. Ripple, Michael J. Carrier, Joshua R. Wayment
Particulates in protein therapeutics, composed of agglomerated protein monomers, may cause an immunogenic response in patients. Consequently, industry and the FDA desire more accurate methods for counting and characterizing particulates. Unlike likely

Predictive Gold Nanocluster Formation Controlled by Metal-Ligand Complexes

January 9, 2012
Author(s)
John M. Pettibone, Jeffrey W. Hudgens
The formation of ligand-protected transition metal nanoclusters in size-selective syntheses is driven by the inherent properties of the protecting ligands. We have previously reported the selective, monodisperse product formation of phosphine protected Au
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