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Health

Pharmaceuticals

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Displaying 51 - 75 of 122

Enhancing the Efficacy of Cancer Therapy: Use of Small Molecule Inhibitors of DNA Glycosylases as Potential Drugs

March 12, 2017
Author(s)
Erdem Coskun, Aaron C. Jacobs, Nathan Donley, Marcus J. Calkins, Dorjbal Dorjsuren, David Maloney, Anton Simeonov, Pawel Jaruga, Amanda K. McCullough, M Miral Dizdar, R. S. Lloyd
Chemotherapy aims to destroy cancer cells by damaging their DNA. However, the overexpression of DNA repair proteins in cancer cells causes the removal of DNA lesions before they become toxic and thus leads to an increased DNA repair capacity resulting in

Structural Calcium (Trypsin, Subtilisin)

February 19, 2017
Author(s)
G L. Gilliland, A Teplyakov
There are about 40 families of serine proteases defined by amino acid sequence similarities that can be further grouped into 7 clans on the basis of their tertiary structure and the order of the catalytic triad. 1 Members of clans SA and SB are

Analytical Gaps and Challenges for Particles in the Submicrometer Size Domain

November 30, 2016
Author(s)
Scott Aldrich, Shawn Cao, Andrea Hawe, Desmond Hunt, Linda Narhi, Dean C. Ripple, Satish Singh
This Stimuli article provides a technical discussion of the available technologies for submicrometer particle analysis, including consideration of the advantages, disadvantages, and technical gaps for each application. These methods can be used in the

Toward Achieving Harmonization in a Nano-cytotoxicity Assay Measurement through an Interlaboratory Comparison Study

September 29, 2016
Author(s)
John T. Elliott, Matthias Roesslein, Nam W. Song, Blaza Toman, Agnieszka Kinsner-Ovaskainen, Rawiwan Maniratanachote, Marc L. Salit, Elijah J. Petersen, Fatima Sequeira, Jieun Lee, Francois Rossi, Cordula Hirsch, Harald Krug, Wongsakorn Suchaoin, Peter Wick
Design and development of reliable cell-based nanotoxicology assays is important for ranking of potential hazardous engineered nanomaterials. Challenges to producing a reliable assay protocol include working with nanoparticle dispersions and living cell

Method Validation and Standards in Hydrogen/Deuterium Exchange Mass Spectrometry

March 1, 2016
Author(s)
Jeffrey W. Hudgens, Richard Y. Huang, Emma D'Ambro
The chapter examines method validation and measurement standards for measuring protein dynamics by hydrogen/deuterium exchange mass spectrometry. Topics examined include the rationale for a reference measurement system for HDX-MS, attaining high accuracy

Protein Particles (0.1 ym to 100 ym)

October 15, 2015
Author(s)
Dean C. Ripple, Linda Narhi
Protein molecules in solution can form proteinaceous particles by a variety of aggregation processes. The size and concentration of these particles is an important quality attribute for therapeutic MAb solutions. In this chapter, we describe the techniques

Sub-visible (2 mm to 100 mm) particle analysis during biotherapeutic drug product development: Part 1, considerations and strategy

April 1, 2015
Author(s)
Dean C. Ripple, Linda Narhi, Natliya Afonina, Satish Singh, Andrea Herre, Patrick Garidel, Atanas Koulov, Vincent Corvari, Thomas Spitznagel, Paolo Mangiagalli, Irene Cecchini, Klaus Wuchner, Tony Lubiniecki, Hanns-Christian Mahler, Roland Schmidt, Alla Polozova, Patricia Cash, Andrew Weiskopf, Douglas Nesta, Mara Rossi, Robert Simler
Measurement and characterization of particles in the 1 µm to 100 µm range (sub-visible particles), including protein aggregates, is an important part of every stage of protein therapeutic development. The tools used and the ways in which the information

Critical Examination of the Colloidal Particle Model of Globular Proteins

February 3, 2015
Author(s)
Prasad S. Sarangapani, Steven D Hudson, Ronald L. Jones, Jack F. Douglas, Jai A. Pathak
Recent studies of globular protein solutions have uniformly adopted a colloidal view of proteins as particles, a perspective that neglects the polymeric primary structure of these biological macromolecules, their intrinsic flexibility and their ability to

The Use of Index-Matched Beads in Optical Particle Counters

January 8, 2015
Author(s)
Dean C. Ripple
Although multiple methods are available for counting and sizing of protein particles over a broad range of sizes, differences in physical properties between common polystyrene-bead reference materials and the actual protein particles of interest result in

High-Relaxivity Superparamagnetic Iron Oxide Nanoworms with Decreased Immune Recognition and Long-Circulating Properties

November 24, 2014
Author(s)
Stephen E. Russek, Guankui Wang, Swetha Inturi, Natalie J. Serkova, Sergey Merkulov, Keith McCrae, Nirmal K. Banda, Dmitri Simberg
One of the core issues of nanotechnology involves masking the foreignness of nanomaterials to enable in vivo longevity and long-term immune evasion. Dextran-coated superparamagnetic iron oxide nanoparticles are very effective magnetic resonance imaging

An Average Enumeration Method of Hyperspectral Imaging Data for Quantitative Evaluation of Medical Device Surface Contamination

September 15, 2014
Author(s)
Hanh Le, Moon Kim, Jeeseong C. Hwang, Yi Yang, Pawneena U-Thainual, Jin Kang, Do-Hyun Kim
We propose a quantification method called Mapped Average Principal component analysis Score (MAPS) to enumerate the contamination coverage on common medical device surfaces. The method was adapted from conventional Principal Component Analysis (PCA) on non

Membrane protein resistance of oligo(ethylene oxide) self-assembled monolayers

July 31, 2014
Author(s)
David J. Vanderah, Marlon L. Walker, David T. Gallagher, Ryan Vierling, Fay Crawshaw
Spectroscopic ellipsometry was used to evaluate the resistance to protein adsorption (RPA) of self- assembled monolayers (SAMs) of HS(CH2)3O(CH2CH2O)6M and [HS(CH2)3CH]2O-(CH2CH2O)6M, where M = CH3 or H, on Au. The SAMs were exposed to fibrinogen, a

Microfluidic Remote Loading for Rapid Single-Step Liposomal Drug Preparation

July 29, 2014
Author(s)
Wyatt N. Vreeland, Renee R. Hood, Don L. DeVoe
Here we report a microfluidic system that enables rapid and efficient remote loading of amphipathic drugs into nanoscale liposomes, combining liposome synthesis and remote drug loading in a continuous integrated process. Unlike established bulk methods for

A Facile Route to the Synthesis of Monodisperse Nanoscale Liposomes Using 3D Microfluidic Hydrodynamic Focusing in a Concentric Capillary Array

May 14, 2014
Author(s)
Wyatt N. Vreeland, Renee R. Hood, Don L. DeVoe, Francisco J. Atencia, Donna M. Omiatek
A novel microscale device has been developed to enable the one-step continuous flow assembly of monodisperse nanoscale liposomes using three-dimensional microfluidic hydrodynamic focusing (3D-MHF) in a radially symmetric capillary array. The 3D-MHF flow

Structural Basis for Inactivation of Giardia lamblia Carbamate Kinase by Disulfiram

April 11, 2014
Author(s)
Andrey Galkin, Liudmila Kulakova, Kap Lim, Catherine Chen, Wei Zhang, Illarion Turko, Osnat Herzberg
Carbamate kinase from Giardia lamblia is an essential enzyme for the survival of the organism. The enzyme catalyzes the final step in the arginine dihydrolase pathway converting ADP and carbamoyl phosphate to ATP and carbamate. We previously reported that

Microfluidic-Enabled of Liposomes Elucidate Size-Dependent Transdermal Transport

March 21, 2014
Author(s)
Wyatt N. Vreeland, Renee R. Hood, Eric Kendall, Don L. DeVoe, Julia Finkel, Mariana Junqueira, Zenaide Quezado
Microfluidic synthesis of small and nearly-monodisperse liposomes is used to investigate the size-dependent passive transdermal transport of nanoscale lipid vesicles. While large liposomes with diameters above 105 nm are found to be excluded from deeper

Pressure-Sensitive Sampling Wands for Homeland Security Applications

December 1, 2013
Author(s)
Matthew E. Staymates, Jennifer R. Verkouteren, Jessica Grandner
This work discusses the use of Force Sensing Resistor (FSR) technology for Homeland Security applications. One area of particular focus is the optimization of wipe sampling of surfaces to facilitate enhanced trace contraband collection. Another focus area
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