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Method Validation and Standards in Hydrogen/Deuterium Exchange Mass Spectrometry



Jeffrey W. Hudgens, Richard Y. Huang, Emma D'Ambro


The chapter examines method validation and measurement standards for measuring protein dynamics by hydrogen/deuterium exchange mass spectrometry. Topics examined include the rationale for a reference measurement system for HDX-MS, attaining high accuracy and precision, improving trueness by reducing systematic error, removing bias, comparison of HDX-MS and HDX-NMR measurements, measurement reproducibility, and useful reference materials. The program for maintaining standards and monitoring performance is outlined.
Hydrogen-Deuterium Exchange Mass Spectrometry: Fundamentals, Techniques and Applications
Publisher Info
John Wiley & Sons, Chichester, -1


Hydrogen/Deuterium Exchange, Mass Spectrometry, Method Validation, Molecular dynamics, Proteins, Standards


Hudgens, J. , Huang, R. and D'Ambro, E. (2016), Method Validation and Standards in Hydrogen/Deuterium Exchange Mass Spectrometry, John Wiley & Sons, Chichester, -1, [online], (Accessed May 24, 2024)


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Created March 1, 2016, Updated December 1, 2017