Method Validation and Standards in Hydrogen/Deuterium Exchange Mass Spectrometry
Jeffrey W. Hudgens, Richard Y. Huang, Emma D'Ambro
The chapter examines method validation and measurement standards for measuring protein dynamics by hydrogen/deuterium exchange mass spectrometry. Topics examined include the rationale for a reference measurement system for HDX-MS, attaining high accuracy and precision, improving trueness by reducing systematic error, removing bias, comparison of HDX-MS and HDX-NMR measurements, measurement reproducibility, and useful reference materials. The program for maintaining standards and monitoring performance is outlined.
Hydrogen-Deuterium Exchange Mass Spectrometry: Fundamentals, Techniques and Applications
, Huang, R.
and D'Ambro, E.
Method Validation and Standards in Hydrogen/Deuterium Exchange Mass Spectrometry, John Wiley & Sons, Chichester, -1, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=915004
(Accessed November 29, 2023)