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Certification of Standard Reference Material(R) 968f Fat-Soluble Vitamins in Frozen Human Serum

December 14, 2017

Author(s)

Jeanice M. Brown Thomas, David L. Duewer, Carolyn Q. Burdette, Lorna T. Sniegoski, James H. Yen

Standard Reference Material (SRM) 968f is intended for use in validating methods for determining fat soluble vitamins in human serum and plasma and value assigning in house produced control materials analyzed using those methods. A unit of SRM 968f

Nanowire Aptasensors for Electrochemical Detection of Cell-Secreted Cytokines

October 9, 2017

Author(s)

Ying Liu, Ali Rahimian, Sergiy Krylyuk, Tam Vu, Bruno Crulhas, Gulnaz Stybayeva, Dong-Sik Shin, Albert Davydov, Alexander Revzin, Meruyert Imanbekova

Cytokines are small proteins secreted by immune cells in response to pathogens/infections; therefore these proteins can be used in diagnosing infectious diseases. For example, release of a cytokine interferon (IFN)-γ from T-cells is used for blood-based

Sensing bacterial vibrations and early response to antibiotics with phase noise of a resonant crystal

September 22, 2017

Author(s)

Ward L. Johnson, Danielle C. France, Nikki S. Rentz, William T. Cordell, Fred L. Walls

A new approach is presented for sensing mechanical fluctuations of populations of bacteria, with potential application in monitoring microbial responses to changes in chemical environment, such as antimicrobial intervention. Bacteria are tethered to a

Baseline assessment of 25-hydroxyvitamin D reference material and proficiency testing/external quality assurance material commutability - a vitamin D standardization program study

August 10, 2017

Author(s)

Karen W. Phinney, Christopher T. Sempos, Hubert W. Vesper, Julianne C. Botelho, M Dasti, Yasamin Rahmani, Susan Tai, Johanna Camara, Stephen A. Wise, Gary L. Myers, Ramon Durazo-Arvizu, Lu Tian, John H. Eckfeldt, Andrew N. Hoofnagle, W. Greg Miller, Lorin M. Bachmann, Ian S. Young, J Pettit, G. Caldwell, A Liu, S P. Brooks, K. Sarafin, M. Thamm, C. Scheidt-Nave, G. B. Mensink, M. Busch, M. Rabenberg, Kevin Cashman, Mairead Kiely, Albert Flynn, Kirsten D. Dowling, B. Quinn, K OH, S. W. Lee, C. L. Jung, L. Cox, G. Goldberg, K. Guberg, S. Meadows, A. Prentice, G. Swan, Alison Tedstone, Schleicher Rosemary, Christine M. Pfeiffer, Graham D. Carter, Julia Jones, P. Brannon, R. M. Lucas, Neil C. Binkley, P. M. Crump, Donald Wiebe, Glenville Jones, J. M. Betz, C. D. Faigen, Joyce Merkel, Elizabeth A. Yetley, Paul M. Coates

The Vitamin D Standardization Program (VDSP) coordinated a study to assess the commutability of reference materials and proficiency testing/external quality assurance materials for total 25-hydroxyvitamin D [25(OH)D] in human serum, the primary indicator

General steps to standardize the laboratory measurement of serum total 25-hydroxyvitamin D

August 1, 2017

Author(s)

Karen W. Phinney, Christopher T. Sempos, J. M. Betz, Johanna Camara, Graham D. Carter, E. Cavalier, Michael W. Clarke, Kirsten D. Dowling, Ramon Durazo-Arvizu, Andrew N. Hoofnagle, A Liu, Kurtis Sarafin, Stephen A. Wise, Paul M. Coates

The Vitamin D Standardization Program (VDSP) has collaborated with numerous groups and agencies to assemble a set of tools, i.e. a reference measurement system, that can be used to establish traceability of 25-hydroxyvitamin D [25(OH)D] assays to relevant

NIST/NIH Vitamin D Metabolites Quality Assurance Program Report of Participant Results: Summer 2016 Comparability Study (Exercise 12)

July 31, 2017

Author(s)

Mary Bedner, Carolyn Q. Burdette

The National Institute of Standards and Technology (NIST) has established a Vitamin D Metabolites Quality Assurance Program (VitDQAP) in collaboration with the National Institutes of Health (NIH) Office of Dietary Supplements. Participants in the twelfth

Principles and Recommendations for Standardizing the Use of the Next Generation Sequencing Variant File in Clinical Settings

March 16, 2017

Author(s)

Justin M. Zook, Ira Lubin, Lisa V. Kalman

A national workgroup convened by the Centers for Disease Control and Prevention identified principles and made recommendations for standardizing the description of sequence data contained within the variant file generated during the course of clinical next

Standardizing CT Lung Density Measure Across Scanner Manufacturers

March 1, 2017

Author(s)

Huaiyu H. Chen-Mayer, Matthew Fuld, Bernice Hoppel, Phillip Judy, Junfeng Guo, David Lynch, Antonio M. Possolo, Sean B. Fain

Computed Tomography imaging of the lung, reported in Hounsfield Units (HU), can be parameterized as a quantitative image biomarker for the diagnosis and monitoring of lung density changes due to emphysema, a type of chronic obstructive pulmonary disease

Development and Characterization of Reference Materials for Genetic Testing

November 1, 2016

Author(s)

Justin M. Zook, Marc L. Salit, Lisa V. Kalman, Mickey Williams, Vivekananda Datta, Jin-Yeong Han

Characterized reference materials (RM) are needed for test development and validation, quality control procedures and proficiency testing to assure the quality of clinical laboratory tests. In this article, we review the development and characterization of

Quantification of cardiac troponin I in human plasma by immunoaffinity enrichment and targeted mass spectrometry

September 30, 2016

Author(s)

Nicole A. Schneck, Karen W. Phinney, Sang Bok Lee, Mark S. Lowenthal

Quantification of cardiac troponin I (cTnI), a protein biomarker used for diagnosing myocardial infarction, has been achieved in native patient plasma based on an immunoaffinity enrichment strategy and isotope dilution (ID) liquid chromatography-tandem

Certified DNA Reference Materials to Compare HER2 Gene Amplification Measurements Using Next- Generation Sequencing Methods

September 1, 2016

Author(s)

Chih-Jian Lih, Robin D. Harrington, Kneshay Harper, David J. Sims, Paul McGregor, Corinne Camalier, Han Si, Biswajit Das, P. M. Williams, Hua-Jun He, Jamie L. Almeida, Steven Lund, Steven J. Choquette, Kenneth D. Cole

The National Institute of Standards and Technology (NIST) Standard Reference Materials 2373 is a set of genomic DNA samples prepared from five breast cancer cell lines with certified values for the ratio of the HER2 gene copy number to the copy numbers of

Comparing Variant Call Files for Performance Benchmarking of Next-Generation Sequencing Variant Calling Pipelines

August 3, 2016

Author(s)

Justin Zook, John G. Cleary, Len Trigg, Francisco De La Vega

To evaluate and compare the performance of variant calling methods and confidence scores, comparisons between a test call set and a "gold standard" need to be carried out. Unfortunately, these comparisons are not straightforward with the current Variant

Standards for cell line authentication and beyond

June 14, 2016

Author(s)

Jamie L. Almeida, Kenneth D. Cole, Anne L. Plant

Different genomic technologies have been applied to cell line authentication, but only one method (short tandem repeat profiling) has been the subject of a comprehensive standard (ASN-0002). Here we discuss the power of this document, and why standards

Wide-field quantitative imaging of tissue microstructure using high-frequency structured light

June 9, 2016

Author(s)

David McClatchy III, Elizabeth Rizzo, wendy wells, Jeeseong C. Hwang, keith paulson, Brian Pogue, Stephen Kanick

Sub-diffusive structured light imaging has been shown to accurately and quantitatively map the reduced scattering coefficient and the phase function backscatter parameter in a wide-field geometry. This study shows the first the experimental imaging of

Extensive sequencing of seven human genomes to characterize benchmark reference materials

June 7, 2016

Author(s)

Justin M. Zook, Jennifer H. McDaniel, David N. Catoe, Lindsay Harris, Marc L. Salit

The Genome in a Bottle Consortium hosted by the National Institute of Standards and Technology, (NIST), is creating reference materials and data for human genome sequencing, as well as methods for genome comparison and benchmarking. Here, we describe a

Assignment of the Number of Equivalent Reference Fluorophores to Dyed Microspheres

June 2, 2016

Author(s)

Adolfas Gaigalas, Lili Wang, Paul C. DeRose

A procedure will be described to assign to each dyed microsphere a number called the Equivalent number of Reference Fluorophores (ERF). The ERF unit gives the number of reference fluorophores in solution which produce the same fluorescence signal as a

High Levels of Gadolinium Deposition in the Skin of a Patient with Normal Renal Function

May 2, 2016

Author(s)

William C. Davis, Donna R. Roberts, Scott M. Lindhorst, Cynthia T. Welsh, Mary N. Herring, Adam Braun, Bruce H. Thiers

Objective: The aim of this study was to assess gadolinium deposition in the skin of a patient with normal renal function, based on estimated glomerular filtration rate (eGFR) values greater than 59 ml/min/1.73 m2, following exposure to large cumulative

Development of NIST Standard Reference Material(R) 2373: Genomic DNA Standards for HER2 Measurements

March 9, 2016

Author(s)

Hua-Jun He, Jamie L. Almeida, Steven Lund, Carolyn R. Steffen, Steven J. Choquette, Kenneth D. Cole

NIST Standard Reference Material (SRM®) 2373 was developed to improve the measurements of the HER2 gene amplification in DNA samples. SRM® 2373 consists of genomic DNA extracted from five breast cancer cell lines with different amounts of amplification of

Clinical Implications of Technical Performance in Medical Genome Sequencing

March 2, 2016

Author(s)

Justin M. Zook, James Priest, Rachel Goldfeder, Megan Grove, Daryl Waggott, Matthew Wheeler, Euan Ashley, Marc L. Salit

As next-generation sequencing is becoming routinely applied to clinical care, the predictive characteristics and limitations of whole exome and whole genome sequencing need to be well-understood. The Genome in a Bottle Consortium has recently published a

Handheld Real-Time PCR Device

January 26, 2016

Author(s)

Christian D. Ahrberg, Robert Ilic, Andreas Manz, Pavel Neu?il

Here we report one of the smallest real-time PCR system up to date with approximate size of 100 mm × 60 mm × 33 mm. The system is an autonomous unit requiring an external 12 V power supply. Four simultaneous reactions are performed in form of virtual

SVClassify: a method to use multiple datasets to classify candidate structural variants into true positives and false positives

January 16, 2016

Author(s)

Justin M. Zook, Hemang M. Parikh, Desu Chen, Hariharan K. Iyer, Marc L. Salit, Wolfgang Losert

The human genome contains variants ranging in size from small single nucleotide polymorphisms (SNPs) to large structural variants (SVs). While high-quality benchmark small variant calls have recently been developed by the Genome in a Bottle Consortium, no

Report from the Standards for Pathogen Identification via Next-Generation Sequencing (SPIN) Workshop

December 1, 2015

Author(s)

Nathanael Olson, Scott Jackson, Nancy Lin

Next-generation sequencing (NGS) is not routinely used in applied settings due to lack of confidence in results. This workshop convened experts to identify measurement challenges impeding NGS implementation and potential standards-based solutions to

HOW WELL DO YOU KNOW YOUR URINE ALBUMIN?

November 24, 2015

Author(s)

Ashley Beasley Green

Quantification of Borrelia burgdorferi membrane proteins in human serum: a new concept for detection of bacterial infection

November 17, 2015

Author(s)

Sao F. Cheung, Kyle Anderson, Kenia Benitez, Mark J. Soloski, John N. Aucott, Karen W. Phinney, Illarion Turko

The low abundance of bacterial proteins in human serum upon infection imposes a challenge for the early proteomic detection of bacterial infection. To address this challenge, we propose to take advantage of detecting membrane proteins released from

Method for the determination of 15N incorporation percentage in labeled peptides and proteins

October 27, 2015

Author(s)

Eric L. Kilpatrick

Use of labeled 15N proteins and peptides as internal standards in isotope-dilution mass spectrometry for the quantification of proteins has been increasing and is now accepted as a gold standard for this analysis. As a necessary reagent in this process