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Handheld Real-Time PCR Device



Christian D. Ahrberg, Robert Ilic, Andreas Manz, Pavel Neu?il


Here we report one of the smallest real-time PCR system up to date with approximate size of 100 mm × 60 mm × 33 mm. The system is an autonomous unit requiring an external 12 V power supply. Four simultaneous reactions are performed in form of virtual reaction chambers (VRC) where a ≈ 100 nL sample is covered with mineral oil and placed on a glass cover slip. Fast, 40 cycle amplification of an amplicon from the H7N9 gene was used to demonstrate PCR performance. The standard curve slope was (-3.02 ± 0.16) CT /dec (mean ± standard deviation) corresponding to an amplification efficiency of (0.91 ± 0.05) per cycle (mean ± standard deviation). The PCR device was capable of detecting a single DNA copy. These results further suggest that our handheld PCR device may have broad, technologically-relevant applications extending to rapid detection of infectious diseases in small clinics.
Lab on A Chip


real-time PCR, hand held, portable, standard curve


Ahrberg, C. , Ilic, R. , Manz, A. and Neu?il, P. (2016), Handheld Real-Time PCR Device, Lab on A Chip, [online],, (Accessed May 23, 2024)


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Created January 25, 2016, Updated October 12, 2021