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Health

Clinical diagnostics

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Displaying 126 - 150 of 158

Quantification of Borrelia burgdorferi membrane proteins in human serum: a new concept for detection of bacterial infection

November 17, 2015
Author(s)
Sao F. Cheung, Kyle Anderson, Kenia Benitez, Mark J. Soloski, John N. Aucott, Karen W. Phinney, Illarion Turko
The low abundance of bacterial proteins in human serum upon infection imposes a challenge for the early proteomic detection of bacterial infection. To address this challenge, we propose to take advantage of detecting membrane proteins released from

Benchmarks for genome sequencing

September 23, 2015
Author(s)
Justin M. Zook, Marc L. Salit
In this issue, Griffith et al. present one of the most comprehensive genome-sequence data sets to date from a patient with myeloid leukemia, including sequencing of normal tissue, primary cancer, and a relapse. Their raw data from a variety of sequencing

Evaluation of two mitochondrial DNA biomarkers for prostate cancer detection

August 17, 2015
Author(s)
Samantha D. Maragh, Steven P. Lund
BACKGROUND: A 3.4kb deletion (3.4kbΔ) in mitochondrial DNA (mtDNA) found in histologically normal prostate biopsy specimens has been reported to be a biomarker for the increased probability of prostate cancer. Increased mtDNA copy number is also reported

Quantifying and Improving Clinical-grade Coverage and Accuracy using Augmented Exome Sequencing

July 16, 2015
Author(s)
Justin M. Zook, Anil Patwardhan, Marc L. Salit, Carlos Bustamante, Euan Ashley, Michael Snyder, John West, Richard Chen
Exome sequencing is increasingly used for the clinical evaluation of genetic disease, yet accuracy and coverage in medically interpretable parts of the genome remains under-characterized. We evaluate recently developed exome sequencing platforms in the

An analytical framework for optimizing variant discovery from personal genomes

February 25, 2015
Author(s)
Justin M. Zook, Gareth Highnam, Jason Wang, Dean Kusler, Vinaya Vijayan, Nir Leibovich, David Mittelman
The standardization and performance testing of analysis tools is a prerequisite to widespread adoption of high-throughput sequencing, particularly in the clinic. However, performance testing is currently complicated by the paucity of standards and

Structure and Function of the Membrane Anchoring Self-Assembled Monolayers

December 14, 2014
Author(s)
David J. Vanderah, Tadas Ragaliauskas, Mindaugas Mickevicius, Mariya Jankunec, Gediminas Niaura, Gintaras Valincius, Bozena Rakovska
Structure of the self-assembled monolayers (SAMs) used to anchor phospholipid bilayers to surfaces affects the functional properties of the tethered bilayer membranes (tBLMs). SAMs of the same surface composition differing in the lateral distribution of

Genomic Reference Materials for Clinical Application

November 21, 2014
Author(s)
Justin M. Zook, Marc L. Salit
Reference Materials are well-characterized, homogeneous, and stable samples that can be used to understand measurement performance. The Genome in a Bottle Consortium is developing whole human genome DNA Reference Materials from large batches of DNA

Multiplexed LC-MS/MS Assay for Urine Albumin

July 24, 2014
Author(s)
Ashley B. Green, David M. Bunk, Karen W. Phinney
Targeted Multiplexed Assay for Urinary Albumin Quantification Ashley Beasley-Green, Nijah M. Burris, David Bunk, Karen Phinney Biomolecular Measurement Division National Institute of Standards and Technology, Gaithersburg, MD ABSTRACT Urinary excretion of

Guidelines for Minimum Information for Publication of Quantitative Digital PCR Experiments

April 9, 2013
Author(s)
Ross J. Haynes, Jim Huggett, Carole Foy, Vladimir Benes, Kerry Emslie, Jeremy Garson, Jan Hellemans, Mikael Kubista, Tania Nolan, Michael Pfaffl, Gregory Shipley, Jo Vandesompele, Carl Wittwer, Stephen Bustin
There is growing interest in the digital polymerase chain reaction (dPCR) as technological progress makes it a practical and increasingly affordable technology. dPCR allows the precise quantification of nucleic acid, facilitating the measurement of small

Out-of-plane Stokes imaging polarimeter for early skin cancer diagnosis

July 1, 2012
Author(s)
P. Ghassemi, Paul Lemaillet, Thomas Germer, J. W. Shupp, S. Venna, M. E. Boisvert, K. E. Flanagan, M. H. Jordan, J. C. Ramella-Roman
Optimal treatment of skin cancer before it metastasizes depends critically on early diagnosis and treatment. Imaging spectroscopy and polarized remittance have been utilized in the past for diagnostic purposes, but valuable information can be also obtained

Controlled transport of superparamagnetic beads with spin-valves

October 7, 2011
Author(s)
Wendy R. Altman, John M. Moreland, Stephen E. Russek, Bruce W. Han, Victor M. Bright
Trapping, release, and transport of individual or ensembles of 2.8 υm superparamagnetic beads functionalized with streptavidin were demonstrated using an addressable and non-volatile array of spin-valve (SV) traps integrated into a microfluidic channel

Recent Advances in Electrochemical Glyco-biosensing

April 8, 2011
Author(s)
Rebecca A. Zangmeister, Germarie Sanchez-Pomales
Biosensors based on electrochemical transduction mechanisms have recently made advances into the field of glycan analysis. These glyco-biosensor assays offer simple, rapid, sensitive and economical approaches to the measurement need for rapid glycan
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