Guidelines for Minimum Information for Publication of Quantitative Digital PCR Experiments

Published

April 9, 2013

Author(s)

Ross J. Haynes, Jim Huggett, Carole Foy, Vladimir Benes, Kerry Emslie, Jeremy Garson, Jan Hellemans, Mikael Kubista, Tania Nolan, Michael Pfaffl, Gregory Shipley, Jo Vandesompele, Carl Wittwer, Stephen Bustin

Abstract

There is growing interest in the digital polymerase chain reaction (dPCR) as technological progress makes it a practical and increasingly affordable technology. dPCR allows the precise quantification of nucleic acid, facilitating the measurement of small percentage differences and quantification of rare variants. dPCR may also be more reproducible and less susceptible to inhibition than qPCR. Consequently, dPCR has the potential to have a substantial impact on research as well as diagnostic applications. However, as with qPCR, the ability to perform robust meaningful experiments requires careful design and adequate controls. To assist independent evaluation of experimental data, comprehensive disclosure of all relevant experimental details is required. To facilitate this process we present the Minimum Information for Publication of Quantitative dPCR Experiments (dMIQE) guidelines. This report addresses known requirements for dPCR that have already been identified during this early stage of its development and commercial implementation. Adoption of these guidelines by the scientific community will help to standardize experimental protocols, maximize efficient utilization of resources and enhance the impact of this promising new technology.

Citation

Clinical Chemistry

Volume

59

Issue

6

Pub Type

Journals

Download Paper

DOI Link

Keywords

Digital PCR, MIQE, dMIQE, dPCR

Citation

Haynes, R. , Huggett, J. , Foy, C. , Benes, V. , Emslie, K. , Garson, J. , Hellemans, J. , Kubista, M. , Nolan, T. , Pfaffl, M. , Shipley, G. , Vandesompele, J. , Wittwer, C. and Bustin, S. (2013), Guidelines for Minimum Information for Publication of Quantitative Digital PCR Experiments, Clinical Chemistry, [online], https://doi.org/10.1373/clinchem.2013.206375 (Accessed December 6, 2024)