Baseline assessment of 25-hydroxyvitamin D reference material and proficiency testing/external quality assurance material commutability - a vitamin D standardization program study

Published

August 10, 2017

Author(s)

Karen W. Phinney, Christopher T. Sempos, Hubert W. Vesper, Julianne C. Botelho, M Dasti, Yasamin Rahmani, Susan Tai, Johanna Camara, Stephen A. Wise, Gary L. Myers, Ramon Durazo-Arvizu, Lu Tian, John H. Eckfeldt, Andrew N. Hoofnagle, W. Greg Miller, Lorin M. Bachmann, Ian S. Young, J Pettit, G. Caldwell, A Liu, S P. Brooks, K. Sarafin, M. Thamm, C. Scheidt-Nave, G. B. Mensink, M. Busch, M. Rabenberg, Kevin Cashman, Mairead Kiely, Albert Flynn, Kirsten D. Dowling, B. Quinn, K OH, S. W. Lee, C. L. Jung, L. Cox, G. Goldberg, K. Guberg, S. Meadows, A. Prentice, G. Swan, Alison Tedstone, Schleicher Rosemary, Christine M. Pfeiffer, Graham D. Carter, Julia Jones, P. Brannon, R. M. Lucas, Neil C. Binkley, P. M. Crump, Donald Wiebe, Glenville Jones, J. M. Betz, C. D. Faigen, Joyce Merkel, Elizabeth A. Yetley, Paul M. Coates

Abstract

The Vitamin D Standardization Program (VDSP) coordinated a study to assess the commutability of reference materials and proficiency testing/external quality assurance materials for total 25-hydroxyvitamin D [25(OH)D] in human serum, the primary indicator of vitamin D status. A set of 50 single donor serum samples as well as 17 reference and proficiency testing/external quality assessment materials were analyzed by participating laboratories that employed either immunoassay or liquid chromatography-mass spectrometry methods for total 25(OH)D. The commutability test materials included National Institute of Standards and Technology standard reference material 972a Vitamin D Metabolites in Human Serum as well as study materials from the College of American Pathologists and the Vitamin D External Quality Assessment Scheme. Study protocols and data analysis procedures were in accordance with Clinical and Laboratory Standards Institute guidelines. The majority of the test materials were found to be commutable with the methods used in this commutability study. These results provide guidance for laboratories needing to choose appropriate reference materials and select proficiency or quality assurance programs and will serve as a foundation for additional VDSP studies.

Citation

Journal of Aoac International

Pub Type

Journals

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Keywords

immunoassay, reference measurement procedure, 25-hydroxyvitamin D, Standard Reference Material, total 25-hydroxyvitamin D, liquid chromatography-tandem mass spectrometry

Citation

Phinney, K. , Sempos, C. , Vesper, H. , Botelho, J. , Dasti, M. , Rahmani, Y. , Tai, S. , Camara, J. , Wise, S. , Myers, G. , Durazo-Arvizu, R. , Tian, L. , Eckfeldt, J. , Hoofnagle, A. , , W. , Bachmann, L. , Young, I. , Pettit, J. , Caldwell, G. , Liu, A. , Brooks, S. , Sarafin, K. , Thamm, M. , Scheidt-Nave, C. , Mensink, G. , Busch, M. , Rabenberg, M. , Cashman, K. , Kiely, M. , Flynn, A. , Dowling, K. , Quinn, B. , OH, K. , Lee, S. , Jung, C. , Cox, L. , Goldberg, G. , Guberg, K. , Meadows, S. , Prentice, A. , Swan, G. , Tedstone, A. , Rosemary, S. , Pfeiffer, C. , Carter, G. , Jones, J. , Brannon, P. , Lucas, R. , Binkley, N. , Crump, P. , Wiebe, D. , Jones, G. , Betz, J. , Faigen, C. , Merkel, J. , Yetley, E. and Coates, P. (2017), Baseline assessment of 25-hydroxyvitamin D reference material and proficiency testing/external quality assurance material commutability - a vitamin D standardization program study, Journal of Aoac International, [online], https://doi.org/10.5740/jaoacint.17-0291 (Accessed October 17, 2025)