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Bioscience

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Displaying 1101 - 1125 of 1280

Gene Expression Analysis on Medium-Density Oligonucleotide Arrays

January 1, 2001
Author(s)
R. Sinibaldi, C D. O'Connell, H Rodriguez, C. Seidel
As the human genome project continues toward its goal of sequencing the entire human genome by the end of 2003, this is providing unique opportunities for studying genetic variation in humans and its relationship with disease risk and aging. Consequently

Estimation of the Hydrophobic Effect in an Antigen-Antibody Protein-Protein Interface

December 19, 2000
Author(s)
E. J. Sundberg, M. Urrutia, B C. Braden, J. Isern, D. Tsuchiya, B. A. Fields, E Malchiodi, J. Tormo, Frederick P. Schwarz, R A. Mariuzza
Antigen-antibody complexes provide useful models for analyzing the thermodynamics of protein-protein association reactions. We have employed site-directed mutagenesis, X-ray crystallography, and isothermal titration calorimetry to investigate the role of

Repair of oxidative DNA damage in Drosophila melanogaster: identification and characterization of dOgg1, a second DNA glycosylase activity for 8-hydroxyguanine and formamidopyrimidines

December 1, 2000
Author(s)
C. Dherin, M. Dizdaroglu, H. Doerflinger, S. Boiteux, J. P. Radicella
In Drosophila, the S3 ribosomal protein has been shown to act as a DNA glycosylase/AP lyase capable of releasing 8-hydroxyguanine (8-OH-Gua) in damaged DNA. Here we describe a second Drosophila protein (dOggl) with 8-OH-Gua and abasic (AP) site DNA repair

Infrared Spectroscopic Ellipsometry of Self-Assembled Monolayers

November 28, 2000
Author(s)
Curtis W. Meuse
Ellipsometry measures the relative intensity of and the phase difference between the parallel (p) and perpendicular (s) components of an electric field vector interacting with a sample. In this paper, a technique using polarized Fourier transform infrared

Stressing-Out DNA? The Contribution of Serine-Phosphodiester Interactions in Catalysis by Uracil DNA Glycosylase

October 17, 2000
Author(s)
R M. Werner, Y. L. Jiang, R. G. Gordley, J. Jagadeesh, Jane E. Ladner, G Xiao, M Tordova, G L. Gilliland, J T. Stivers
The DNA repair enzyme uracil DNA glycosylase (UDG) pinches the phosphodiester backbone of damaged DNA using the hydroxyl side chains of a conserved trio of serine residues, resulting in flipping of the deoxyuridine from the DNA helix into the enzyme active

Prokaryotic Expression, Purification, and Reconstitution of Biological Activities (Antiprotease, Antitumor and Heparin-Binding) for Tissue Factor Pathway Inhibitor- 2

October 5, 2000
Author(s)
C N. Rao, Prasad T. Reddy, D J. Reeder, Yonglin Liu, S M. Stack, W Kisiel, D T. Woodley
We report the expression of tissue factor pathway inhibitor-2 (TFPI-2) (also known as PP-5, placental protein-5; MSPI, matrix-associated serine protease inhibitor) in E. coli as a 25 kDa non-glycosylated protein with a glycine substituted for aspartic acid

The Effect of an Oxidized Gold Substrate on Alkanethiol Self-Assembly

June 1, 2000
Author(s)
John T. Woodward IV, Marlon L. Walker, Curtis W. Meuse, David J. Vanderah, G Poirier, Anne L. Plant
UV cleaned gold substrates incubated in solutions of alkanethiol show islands on the monolayer surface when imaged with non-contact atomic force microscopy (AFM). The height of the islands above the monolayer is approximately twice the height of the

Effective Fragment Potentials and the Enzyme Active Site

May 1, 2000
Author(s)
S E. Worthington, Morris Krauss
Optimization of the binding conformation of a substrate in an enzyme active site using ab initio quantum chemistry methods are intractable since the active site comprises several hundred atoms. However, the active site can be decomposed into an active and
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