NOTICE: Due to a lapse in annual appropriations, most of this website is not being updated. Learn more.

Form submissions will still be accepted but will not receive responses at this time. Sections of this site for programs using non-appropriated funds (such as NVLAP) or those that are excepted from the shutdown (such as CHIPS and NVD) will continue to be updated.

U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PUBLICATIONS

Genetically Engineered Metal Ion Binding Sites on the Outside of a Channel's Transmembrane Β-Barrel

Published

Author(s)

John J. Kasianowicz, D L. Burden, L C. Han, S Cheley, H Bayley

Abstract

We are exploring the ability of genetically engineered versions of the Staphlococcus aureus αHL} ion channel to serve as rationally designed sensor components for analytes including divalent cations. We show here that neither the hemolytic activity nor the single channel current of wild type αHL were affected by [Zn(II)]
Citation
Biophysical Journal
Volume
76
Issue
2
Pub Type
Journals

Keywords

alpha-hemolysin, alpha-toxin, biosensor, divalent cations, ion channels, protein structure, transition metals
Bioscience and Chemical thermodynamics and chemical properties

Citation

Kasianowicz, J. , Burden, D. , Han, L. , Cheley, S. and Bayley, H. (1999), Genetically Engineered Metal Ion Binding Sites on the Outside of a Channel's Transmembrane Β-Barrel, Biophysical Journal (Accessed October 27, 2025)

Issues

If you have any questions about this publication or are having problems accessing it, please contact [email protected].

Created February 1, 1999, Updated February 19, 2017
Was this page helpful?