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Genetically Engineered Metal Ion Binding Sites on the Outside of a Channel's Transmembrane Β-Barrel

Published

Author(s)

John J. Kasianowicz, D L. Burden, L C. Han, S Cheley, H Bayley

Abstract

We are exploring the ability of genetically engineered versions of the Staphlococcus aureus αHL} ion channel to serve as rationally designed sensor components for analytes including divalent cations. We show here that neither the hemolytic activity nor the single channel current of wild type αHL were affected by [Zn(II)]
Citation
Biophysical Journal
Volume
76
Issue
2

Keywords

alpha-hemolysin, alpha-toxin, biosensor, divalent cations, ion channels, protein structure, transition metals

Citation

Kasianowicz, J. , Burden, D. , Han, L. , Cheley, S. and Bayley, H. (1999), Genetically Engineered Metal Ion Binding Sites on the Outside of a Channel's Transmembrane Β-Barrel, Biophysical Journal (Accessed May 25, 2024)

Issues

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Created February 1, 1999, Updated February 19, 2017