Results from the 1999 NIST Mixed-Stain Study #2 DNA Quantitation, Differential Extraction and Identification of the Unknown Contributors

Published

September 1, 1999

Author(s)

Margaret C. Kline, Janette W. Redman, David L. Duewer, D J. Reeder

Abstract

The National Institute of Standards and Technology (NIST) has provided a number of educational interlaboratory exercises to the forensic community. The recent NIST Mixed Stain Study #2 consisted of three separate sets of materials. Set 1) three stains on washed cotton sheeting, representing a sexual assault by an unknown assailant; a blood stain from the known female victim, a neat semen stain from a known suspect, and a mixed blood/semen stain of the above knowns plus an additional unknown semen specimen. Set 2) three stains on washed cotton sheeting, representing a sexual assault; a bloodstain from the known female victim, a neat semen stain from a known suspect, and a mixed blood/semen stain where the semen was different from the male reference. Set 3) five 20 L DNA TE buffer extracts in screw-capped vials with DNA concentrations stated to range from approximately equal to} 0.2 to approximately equal to} 20 ng/ L. Two of these Set 3 samples were, in fact, replicates.

Proceedings Title

10th International Symposium on Human Identification - 1999

Conference Dates

September 1, 1999

Conference Location

Orlando, FL

Pub Type

Conferences

Keywords

DNA, DNA analysis, DNA concentrations, forensics

Citation

Kline, M. , Redman, J. , Duewer, D. and Reeder, D. (1999), Results from the 1999 NIST Mixed-Stain Study #2 DNA Quantitation, Differential Extraction and Identification of the Unknown Contributors, 10th International Symposium on Human Identification - 1999, Orlando, FL (Accessed October 8, 2025)