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Bioscience

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Displaying 1126 - 1150 of 1280

Differential Surface Stress of a Tin Oxide Electrode

April 1, 2000
Author(s)
Gintaras Valincius, Vytautas Reipa
Differential surface stress measurements performed using estance technique and simultaneous measurements of the differential capacitance provide experimental evidence for deviation of n-tin oxide electrode's properties from the electrocapillary

Human Mitochondrial Genetics

January 1, 2000
Author(s)
L A. Tully, Barbara C. Levin
The field of human mitochondrial genetics has advanced way beyond where the Human Genome Project hopes to be by the year 2003, the projected year for completing the sequence of the entire human nuclear DNA genome. Not only has the mitochondrial DNA (mtDNA)

Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules

December 1, 1999
Author(s)
M Akeson, D Branton, John J. Kasianowicz, E Brandin, D W. Deamer
Single molecules of DNA or RNA can be detected as they are driven through a single alpha-hemolysin channel by an applied electric field. During translocation, nucleotides within the polynucleotide must pass through the channel pore in sequential single

Challenges in Capturing Oxygenase Activity In Vitro

November 1, 1999
Author(s)
V L. Vilker, Vytautas Reipa, M P. Mayhew, Marcia J. Holden
Biocatalysis using oxygenase or desaturase enzymes has the potential to add value to native fats and oils by adding oxygen hydroxyl groups, or double bonds to create regio- and/or stereospecific products. These enzymes are a subset of the large class of

Heteronuclear NMR and Crystallographic Studies of Wild-Type and H187Q Escherichia Coli Uracil DNA Glycosylase: Electrophilic Catalysis of Uracil Expulsion by a Neutral Histidine 187

September 14, 1999
Author(s)
A C. Drohat, G Xiao, M Tordova, J. Jagadeesh, K Pankiewicz, K A. Watanbe, G L. Gilliland, J T. Stivers
The nature of the putative general acid, His187, in the reaction catalyzed by Escherichia coli uracil DNA glycosylase (UDG) was investigated using X-ray crystallography and NMR spectroscopy. The crystal structures of H187Q UDG, and its complex with uracil
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