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Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules

Published

Author(s)

M Akeson, D Branton, John J. Kasianowicz, E Brandin, D W. Deamer

Abstract

Single molecules of DNA or RNA can be detected as they are driven through a single alpha-hemolysin channel by an applied electric field. During translocation, nucleotides within the polynucleotide must pass through the channel pore in sequential single-file order because the limiting diameter of the pore can accommodate only one strand of DNA or RNA at a time. Here we demonstrate that this nanopore detector can rapidly discriminate between pyrimidine and purine segments along single RNA molecules. Nanopore detection and characterization of single molecules represents a new method for directly reading information encoded in linear polymers, and is a critical first step toward direct sequencing of individual DNA and RNA molecules.
Citation
Biophysical Journal
Volume
77
Issue
6

Keywords

DNA, ion channels, sequencing

Citation

Akeson, M. , Branton, D. , Kasianowicz, J. , Brandin, E. and Deamer, D. (1999), Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules, Biophysical Journal (Accessed February 20, 2024)
Created November 30, 1999, Updated October 12, 2021