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Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules
Published
Author(s)
M Akeson, D Branton, John J. Kasianowicz, E Brandin, D W. Deamer
Abstract
Single molecules of DNA or RNA can be detected as they are driven through a single alpha-hemolysin channel by an applied electric field. During translocation, nucleotides within the polynucleotide must pass through the channel pore in sequential single-file order because the limiting diameter of the pore can accommodate only one strand of DNA or RNA at a time. Here we demonstrate that this nanopore detector can rapidly discriminate between pyrimidine and purine segments along single RNA molecules. Nanopore detection and characterization of single molecules represents a new method for directly reading information encoded in linear polymers, and is a critical first step toward direct sequencing of individual DNA and RNA molecules.
Akeson, M.
, Branton, D.
, Kasianowicz, J.
, Brandin, E.
and Deamer, D.
(1999),
Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules, Biophysical Journal
(Accessed October 7, 2025)