Challenges in Capturing Oxygenase Activity In Vitro

Published

November 1, 1999

Author(s)

V L. Vilker, Vytautas Reipa, M P. Mayhew, Marcia J. Holden

Abstract

Biocatalysis using oxygenase or desaturase enzymes has the potential to add value to native fats and oils by adding oxygen hydroxyl groups, or double bonds to create regio- and/or stereospecific products. These enzymes are a subset of the large class of oxidoreductase enzymes (from EC sub-groups 1.13 and 1.14) involved with biological oxidation and reduction. In vitro biocatalytic processing using these enzymes is hampered by the high cost of the stoichiometric cofactors. This article reviews recent progress in developing in vitro redox enzyme biocatalysis for commercial-scale synthesies. Coenzyme recycling and electrochemical redox cycling as methods for co-factor regeneration are described and commercial applications indicated. Direct charge transfer without use of mediators is described as the cleanest way of introducing the reducing power into the catalytic cycle. Our electrochemically driven cytochrome P450_cam bioreactor is discussed as an example of direct charge transfer to a redox protein. We also show that site-directed mutagenesis in the active site of the P450_cam monooxygenase greatly improved performance for the conversion of the nonnative substrate, styrene to styrene oxide. This epoxidation reaction was also shown to give a single product (styrene oxide) in the bioelectrochemical reactor when the diatomic oxygen co-substrate was managed properly.

Citation

Journal of the American Oil Chemists Society

Volume

76

Issue

11

Pub Type

Journals

Keywords

biocatalysis, cytochrome P450, electroenzymology, styrene

Citation

Vilker, V. , Reipa, V. , Mayhew, M. and Holden, M. (1999), Challenges in Capturing Oxygenase Activity In Vitro, Journal of the American Oil Chemists Society (Accessed May 2, 2025)