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Bioscience

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Displaying 1001 - 1025 of 1261

High-Throughput Analysis of Telomerase by Capillary Electrophoresis

January 1, 2003
Author(s)
Donald H. Atha, K Miller, A D. Sanow, J Xu, J L. Hess, O C. Wu, Wenhua Wang, S Srivastava, W E. Highsmith
The enzyme telomerase is expressed in 85-90% of all human cancers, but not in normal, non-stem cell somatic tissues. Clinical assays for telomerase in easily obtained body fluids would have great utility as non-invasive, cost-effective methods for the

Oxidative DNA Damage Biomarkers Used in Tissue Engineered Skin

January 1, 2003
Author(s)
H Rodriguez, Pawel Jaruga, M Birincioglu, Peter E. Barker, C D. O'Connell, M. Dizdaroglu
To assure that tissue engineered medical products are free of genetic changes that might occur from inflammation during the development phase of the product, our laboratory is responding to the need for test methods used to assess the safety and

Local Protein Dynamics and Catalysis: Detection of Segmental Motion Associated With Rate-Limiting Product Release by a Glutathione Transferase

December 24, 2002
Author(s)
S G. Codreanu, Jane E. Ladner, G Xiao, N V. Stourman, D L. Hachey, G L. Gilliland, R N. Armstrong
Glutathione transferase rGSTM1-1 catalyzes the addition of glutathione (GSH) to 1-chloro-2,4-dinitrobenzene in which chemical step of the reaction is 60-fold faster than the physical step of product release. The hydroxyl group of Y115, located in the

Design and Use of a Peptide Nucleic Acid for Detection of the Heteroplasmic Low-Frequency Mitochondrial Encephalomyopathy, Lactic Acidosis, and Stroke-Like Episodes (MELAS) Mutation in Human Mitochondrial DNA

December 1, 2002
Author(s)
Diane K. Hancock, Frederick P. Schwarz, F H. Song, L J. Wong, Barbara C. Levin
Most pathogenic human mitochondrial DNA (mtDNA) mutations are Heteroplasmic and are difficult to detect when present at very low levels in a population of normal mtDNA molecules. This paper describes a simple methodology to detect low levels of the single

Thermodynamic Quantities for the Ionization Reactions of Buffers in Water

December 1, 2002
Author(s)
Robert N. Goldberg, N Kishore, R M. Lennen
This table contains selected values for the pK, standard molar enthalpy of reaction {Δ} rH , and standard molar heat-capacity change {Δ} rC p for the ionization reactions of 64 buffers many of which are relevant to biochemistry and to biology. The values

A New NIST Mitochondrial DNA Interactive Website

August 1, 2002
Author(s)
Barbara C. Levin, L A. Tully, Diane K. Hancock, Frederick P. Schwarz, M S. Lee
Every human cell has from a few dozen to several thousand mitochondria each of which contain mitochondrial DNA (mtDNA). The sequence of the entire human mtDNA (16,569 base pairs) was determined and published by Anderson et al. in 1981. A mtDNA standard

Cell Membrane Hybrid Bilayers Containing the G-Protein-Coupled Receptor CCR5

August 1, 2002
Author(s)
N M. Rao, Vitalii I. Silin, K D. Ridge, John T. Woodward IV, Anne L. Plant
A hybrid bilayer membrane is a planar model membrane that is formed at an alkanethiol monolayer-coated gold surface by the spontaneous reorganization of phospholipid vesicles. Membrane vesicles from monkey kidney COS-cells also reorganize at an alkanethiol

Electrophoretic Capture of Circular DNA in Gels - A Review

August 1, 2002
Author(s)
B. Akerman, Kenneth D. Cole
Results on electrophoretic capture of circular DNA in porous gels are reviewed. Processes, which cause arrest of circular forms of DNA during electrophoresis, can provide a very efficient separation mechanism for the purification of plasmids and bacterial

Grafting Segments from the Extracellular Surface of CCR5 onto a Bacteriorhodopsin Transmembrane Scaffold Confers HIV-1 Corcceptor Activity

April 1, 2002
Author(s)
N G. Abdulaev, T T. Strassmaier, T Ngo, R W. Chen, H Lueke, D D. Oprian, K D. Ridge
The G-protein coupled receptor CCR5 binds three distinct B-chemokines on the solvent exposed segments of the extracellular surface. This same region of CCr5 also interacts with certain macrophage-tropic strains of HIV-1. To investigate structural features

Synchrotron White-Beam X-Ray Topography of Ribonuclease S Crystals

April 1, 2002
Author(s)
W M. Vetter, David Travis Gallagher, M Dudley
With careful experimental design indexed synchrotron white-beam X-ray topographs of ribonuclease S crystals at ambient temperature could be recorded with a definition and contrast comparable to that of monochromatic beam topographs of other proteins

Identification and Characterization of a Human DNA Glycosylase for Repair of Modified Bases in Oxidatively Damaged DNA

March 19, 2002
Author(s)
T. K. Hazra, T. Izumi, I Boldough, B Imhoff, Y W. Kow, Pawel Jaruga, Miral M. Dizdar, Somenath Mitra
8-oxoguanine (8-oxoG), ring-opened purines (formamidopyrimidines or Fapys), and other oxidized DNA base lesions generated by reactive oxygen species, are often mutagenic and toxic, and have been implicated in the etiology of many diseases including cancer

Cytonectin Expression in Alzheimer's Disease

March 1, 2002
Author(s)
S J. Anderson, Peter E. Barker, M G. Hadfield
Cytonectin is a novel 35,000 molecular weight protein that displays remarkable ion-independent adherence properties. This consigns it to a family of well-known adherence molecules essential for cell communication and the development of 3-dimensional tissue
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