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Grafting Segments from the Extracellular Surface of CCR5 onto a Bacteriorhodopsin Transmembrane Scaffold Confers HIV-1 Corcceptor Activity

Published

Author(s)

N G. Abdulaev, T T. Strassmaier, T Ngo, R W. Chen, H Lueke, D D. Oprian, K D. Ridge

Abstract

The G-protein coupled receptor CCR5 binds three distinct B-chemokines on the solvent exposed segments of the extracellular surface. This same region of CCr5 also interacts with certain macrophage-tropic strains of HIV-1. To investigate structural features of CCR5 that contribute toward HIV-1 coreceptor activity, the amino-terminal and extracellular loop segments of CCR5 were linked to form contiguous polypeptides or grafted onto a seven transmembrane helix bacteriorhodopsin (bR) scaffold either singly, or in combination, to produce a series of CCR5/bR chimeras. The CCR5 extracellular surface polypeptides and CCR5/bR chimeras were expressed in E. coli or COS-1 cells and tested for cell surface expression, all trans retinal binding, and/or CD4 dependent env mediated coreceptor activity. The results show that some the CCR5/bR chimeras are transported to the cell surface and form a bR-like chromophore will all-trans retinal. Further, env mediated cell fusion assays identified structural features in the amino-terminal region of the CCR5/bR chimeras that are sufficient for interaction with JRFL env. Mutations at tyrosine rediuses 10 and 14 in the amino-terminal segment of CCR5, persumed tyrosyl sulfation sites, markedly reduced coreceptor activity. A chimera containing the entire extracellular surface of CCR5 grafted onto the bR scaffold exhibited all-trans retinal dependent coreceptor activity. Thus, the approach of using bR as a seven transmembrane helix scaffold may offer an alternative strategy for studying specific receptor-ligand/viral interactions in other G-protein coupled receptors.
Citation
Structure
Volume
10
Issue
4

Keywords

AIDS, chemokine, GPCR, membrane protein, receptor

Citation

Abdulaev, N. , Strassmaier, T. , Ngo, T. , Chen, R. , Lueke, H. , Oprian, D. and Ridge, K. (2002), Grafting Segments from the Extracellular Surface of CCR5 onto a Bacteriorhodopsin Transmembrane Scaffold Confers HIV-1 Corcceptor Activity, Structure (Accessed February 26, 2024)
Created March 31, 2002, Updated October 12, 2021